Publications by authors named "Gonzalo Bustos Quevedo"
Article Synopsis
- Extracellular vesicles (EVs) show promise as biomarkers for liquid biopsy, but current methods struggle to isolate them effectively from complex biofluids like plasma and serum.
- Traditional isolation techniques often lead to contamination with lipoproteins, making it difficult to accurately assess the number and content of EVs.
- The introduction of Fast Protein Liquid Chromatography (FPLC) with Superose 6 offers a better solution to isolate small EVs while minimizing contamination, highlighting their potential for detecting disease-associated biomarkers, especially in pancreatic cancer patients.
Paclitaxel, a widely used cancer chemotherapeutic agent, has high incidence of neurotoxicity associated with the production of neuropathic pain, for which only duloxetine has shown significant but moderate analgesic effect. Since statins, classically used to reduce hypercholesterolemia, have shown antinociceptive effect in preclinical studies on neuropathic pain, we studied whether the antinociceptive efficacy of duloxetine could be synergistically potentiated by rosuvastatin in a model of paclitaxel-induced neuropathy in mice. The astrocytic and microglial responses in the spinal cord of paclitaxel-treated mice were also assessed by measuring GFAP and CD11b proteins, respectively.
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