Adaptation of the binding domain of S-layer protein as a molecular tag for affinity chromatography development.

Introduction: The S-layer proteins are a class of self-assembling proteins that form bi-dimensional lattices named S-Layer on the cell surface of bacteria and archaea. The protein SlpA, which is the major constituent of the S-layer, contains in its C-terminus region (SlpA), a protein domain (named here as SLAP) responsible for the association of SlpA to the bacterial surface. SLAP was adapted for the development of a novel affinity chromatography method: the SLAP-based affinity chromatography (SAC).