Computational mining and redesign of superoxide dismutase with activity-thermostability improvement.

Superoxide dismutase (SOD) is a redox metalloenzyme that serves as a critical defense against oxidative damage caused by reactive oxygen species, with applications across food, cosmetic, and pharmaceutical industries. However, the limited catalytic activity and poor thermostability restrict its effectiveness in industrial processes. Herein, we report a SOD from Deinococcus wulumuqiensis R12 (SOD(R12)) that demonstrates remarkable catalytic activity of 1356.

Electrochemiluminescence Mechanisms and Bioanalysis Based on Multishape Gold Nanoparticles and Visualized ATT-Au NCs.

Herein, a visual electrochemiluminescence (ECL) luminophore, 6-azido-2-thioxanthine-coated gold nanoclusters (ATT-Au NCs), was prepared efficiently in a single step, followed by comprehensive characterization of their structural, optical, and ECL properties using diverse analytical methodologies. Concurrently, gold nanoparticles, gold dimers, gold nanorod (Au NR) dispersions, and gold nanorod dimers (parallel and perpendicular conformations) were synthesized via chemical reduction, DNA ligation, seed growth, and electrostatic adsorption of organic ligands, respectively. The finite difference time domain (FDTD) modeling was subsequently employed to analyze the electromagnetic field distribution surrounding these gold nanoparticles, revealing that parallel gold nanorod dimers notably enhanced the electromagnetic field intensity.

Dual-signal ratiometric electrochemiluminescence biosensor based on Au NPs-induced low-potential emission of PFO Pdots and LSPR-ECL mechanism for ultra-sensitive detection of microRNA-141.

In this study, we have for the first time constructed a ratiometric ECL biosensor for the ultrasensitive detection of microRNAs (miRNAs) using gold nanoparticles (Au NPs) to trigger both the low-potential emission from conjugated polymer poly(9,9-dioctylfluorene-2,7-diyl) dots (PFO Pdots) and the LSPR-ECL effect with sulfur-doped boron nitride quantum dots (S-BN QDs). PFO Pdots were first applied to the Au NPs-modified electrode, followed by covalent binding to capture the hairpin H1. Immediately thereafter, a small amount of miRNA-141 was able to generate a large amount of output DNA (OP) by traversing the target cycle.

Tripedal DNA Walker as a Signal Amplifier Combined with a Potential-Resolved Multicolor Electrochemiluminescence Strategy for Ultrasensitive Detection of Prostate Cancer Staging Indicators.

A multicolor electrochemiluminescence (ECL) biosensor based on a closed bipolar electrode (BPE) array was proposed for the rapid and intuitive analysis of three prostate cancer staging indicators. First, [Irpic-OMe], [Ir(ppy)(acac)], and [Ru(bpy)] were applied as blue, green, and red ECL emitters, respectively, whose mixed ECL emission colors covered the whole visible region by varying the applied voltages. Afterward, we designed a simple Mg-dependent DNAzyme (MNAzyme)-driven tripedal DNA walker (TD walker) to release three output DNAs.