[Newly records of medicinal species in Jiangxi].

Seven species were reported as new records of Jiangxi province, which collected from Wugongshan region, including Huperzia kunmingensis(Lycopodiaceae), Hydrangea mangshanensis(Saxifragaceae), Itea glutinosa(Saxifragaceae), Stellaria monosperma var. japonica(Lycopodiaceae), Youngia pratti(Compositae), and Calanthe henryi(Orchidaceae), Collabium formosanum(Orchidaceae). Among these species, H.

[Effects of Climatic Factors on Contents of Major Medical Components in Smilax china from Different Regions in Western Hunan].

Objective: To study the impact of climatic factors on the major medical component in Smilax china, and to supply a scientific and standard operation protocol on the introduction and cultivation.

Methods: Detect the content of major medical components, such as baicalin and astibin, in Smilax china from eight counties of Xiangxi Autonomous Prefecture. And establish their relationship with annual average temperature, Jan average temp, Jul average temp, valid accumulative temp (≥ 10 degress C), annual maximum temp, annual minimum temp, annual precipitation, annual sunshine amount, non-frost period and relative humidity by using the methods of partial least squares regression analysis (PLS).

The new steroidal glycosides from the aerial parts of Lepidogrammitis drymoglossoides.

Two new steroidal glycosides ponasteroside C (1) and ponasteroside D (2) were isolated from the aerial parts of Lepidogrammitis drymoglossoides. Their structures were elucidated by various spectroscopic techniques (IR, HRESIMS, 1D and 2D NMR). All compounds were evaluated for their cytotoxicity against HeLa and HCT-8 cell lines, and compounds 1 and 2 showed mild activity against all the test cell lines.

[Determination of artemisinic acid in Artemisia annua at different growth stages based on spot area].

Objective: Based on the important medicinal applications of artemisinic acid and the superiority of Thin Layer Chromagraphy (TLC), the spot area method of TLC was presented to determine the content changes of artemisinic acid of Artemisia annua at different growing stages.

Methods: The separation conditions including chromatographic solutions and chromogenic agent were optimized. The detection limit and the linear concentration range were analyzed.

[Variation of content of artemisic acid in different growth stages of Artemisia annua].

Objective: To study the variation of content of Artemisic acid of Artemisia annua from eight areas of four provinces around Wuling Mountain.

Methods: Artemisic acid of plants were extracted by organic solvent method. Qualitative and quantitative analysis of Artemisic acid were measured by thin layer chromatography (TLC) and high performance thin layer chromatography (HPLC), respectively.

[Effects of organic solvents on proliferation of Hela cells line].

Objective: To research the effects of organic solvents on proliferation inhibition of Hela cells line.

Methods: The apoptosis of Hela cells induced by ethanol and dimethylsulfoxide (DMSO) were studied to analyse the error analyse from background organic solvents. The apoptosis of Hela cells induced by ethanol, DMSO and the combination of these two organic solvents with different concentrations were observed by MTT test and Giemsa staining.

[Condition optimization experiment of microwave extaction of flavonoids in rhizome of Drynaria fortunei].

The experiment extracted flavonoids in rhizome of Drynaria fortunei by microwave extraction, and determined the extraction rate through colorimetry. Through the single factor experiment and orthogonal method, the optimum extraction conditions were as follows: ethanol concentration was 40%, solid-liquid ratio was 1:20 (g/mL), microwave power was 325 W, extraction time was 40 s. Under these conditions, the extraction rate reached 1.

[Oil content of Pyracantha seeds and its relations to soil nutrients in northwestern Hunan Province].

Pyracantha seeds were collected from five habitats in the Karst region of northwestern Hunan Province, and the oil content and its components were determined, with their relations to soil nutrients studied. The results showed that soil total P had positive correlation with the linolic acid in P. fortuneana seed oil, while other soil nutrients had no correlations with the fat acids in the oil.