Technetium-99m-labeled macroaggregated albumin lung perfusion scan for diagnosis of hepatopulmonary syndrome: A prospective study comparing brain uptake and whole-body uptake.

Background: Hepatopulmonary syndrome (HPS) is an arterial oxygenation defect induced by intrapulmonary vascular dilatation (IPVD) in the setting of liver disease and/or portal hypertension. This syndrome occurs most often in cirrhotic patients (4%-32%) and has been shown to be detrimental to functional status, quality of life, and survival. The diagnosis of HPS in the setting of liver disease and/or portal hypertension requires the demonstration of IPVD (.

[Stable expression of human thyrotropin receptor in HEK 293T cells].

Objective: To establish a strain of eukaryotic cells with overexpressed human thyrotropin receptor (hTSHR) for the development of anti-thyroid drugs.

Methods: GV266 vector and hTSHR gene were digested by Age I/Nhe I. Plasmids expressing GV266-hTSHR were constructed using T4 ligase and then transfected into 293T cells.

[TgAb interference with experimental Tg values detected by dilution curves].

Objective: To identify the interference of endogenous antithyroglobulin (TgAb) with experimental thyroglobulin (Tg) values using dilution curves.

Methods: Dilution buffer, detectable TgAb serums (TgAb 20-25 IU/mL, Tg free) and undetectable TgAb serums (TgAb < 10 IU/mL, Tg free) were employed to dilute the Tg serums from patients with DTC, respectively. The Tg values were detected by immunometric assay (IMA).

[The correlation between endogenous TgAb and experimental Tg values in patients with differentiated thyroid carcinomas].

Objective: To determine the interference of antithyroglobulin (TgAb) on the measurement of serum thyroglobulin (Tg) in patients with differentiated thyroid carcinomas (DTC).

Methods: The Tg positive serum and TgAb positive serum were taken from the patients with total thyroidectomy and thyroid remnant ablation by radioiodine. The Tg positive serum contained high level of Tg (>10 microg/L) and nondetectable TgAb.

[The effects of antisense oligonucleotides bcl-2/bcl-xl and bcl-2 on proliferation and apoptosis of breast cancer cells].

Objective: To evaluate the effects of phosphorothioate antisense oligonucleotides (ASON) bcl-2/ bc-xl ASON and bcl-2 on the proliferation and apoptosis of breast cancer cells, MCF-7.

Methods: 1) bcl-2 ASON and bcl-2/bcl-xl ASON were transfected into MCF-7 cells with anionic long circulating liposomes (NA), cationic LCL (PA), respectively. 2) After incubation with bcl-2 ASON (FB1), bcl-2/bcl-xl ASON (FB2), NA loaded with bcl-2 ASON (NA-S) or bcl-2/bcl-xl ASON(NA-D), PA loaded with bcl-2 ASON(PA-S) or bcl-2/bc-xl ASON (PA-D) for 24 h, their inhibition on MCF-7 cells were evaluated by using HE staining, methythiazolyltetrazolium (MTT) and flow cytometry (FCM).

[Preparation of 125I-oligonucleotide-long-circulation liposomes].

Objective: To investigate the preparation and the encapsulation efficiency of 125I-oligonucleotide(ON)-long-circulation liposomes (LCL).

Methods: (1) Oligonucleotide was labeled with 125I using thallium chloride tetrahydrate (TICl3) as an oxidizing agent. 125I-oligonucleotide was separated from free 125I by Sephadex G25 column chromatography.