Publications by authors named "Gong Zi-Chen"
Lactic acid promotes metastatic niche formation in bone metastasis of colorectal cancer.
Cell Commun Signal
January 2021
Background: To investigate the effect of lactic acid (LA) on the progression of bone metastasis from colorectal cancer (CRC) and its regulatory effects on primary CD115 (+) osteoclast (OC) precursors.
Methods: The BrdU assay, Annexin-V/PI assay, TRAP staining and immunofluorescence were performed to explore the effect of LA on the proliferation, apoptosis and differentiation of OC precursors in vitro and in vivo. Flow cytometry was performed to sort primary osteoclast precursors and CD4(+) T cells and to analyze the change in the expression of target proteins in osteoclast precursors.
Article Synopsis
- - Bone metastasis from colorectal cancer (CRC) causes the destruction of bone tissue, primarily due to the abnormal activation of osteoclasts, which are responsible for bone resorption.
- - The study found that CRC cells enhance bone resorption by influencing bone marrow-derived monocytes (BMMs) to differentiate into osteoclasts, with an important role played by CCL3, a protein that regulates this process.
- - CRC-derived EGF (epidermal growth factor) triggers the production of CCL3 in BMMs through a specific signaling pathway, and blocking either EGF or CCL3 can significantly reduce bone degradation associated with CRC metastasis.