Trichinella spiralis: low vaccine potential of glutathione S-transferase against infections in mice.

We have previously reported that Trichinella spiralis glutathione-S-transferase (TsGST) gene is an up-regulated gene in intestinal infective larvae (IIL) compared to muscle larvae (ML). In this study, the TsGST gene was cloned, and recombinant TsGST (rTsGST) was produced. Anti-rTsGST serum recognized the native TsGST by Western blotting in crude antigens of ML, adult worm (AW) and newborn larvae (NBL) of T.

[mRNA expression level of nucleostemin in esophageal squamous cell carcinoma tissue].

Objective: To investigate the mRNA expression levels of nucleostemin (NS) in human esophageal squamous cell carcinoma tissue.

Methods: Real-time PCR was used to quantify the mRNA expression of NS in the samples of esophageal squamous cell carcinoma tissue and their matched normal esophageal mucosa tissue from 62 patients, 36 males and 26 females, aged (61 +/- 10) (38-75). The relationship between NS mRNA expression level and clinical pathological features was analyzed.

[Expression of nucleostemin mRNA and protein in the esophageal squamous cell carcinoma].

Objective: To investigate the mRNA and protein expression of nucleostemin (NS) in human esophageal squamous cell carcinoma.

Methods: The mRNA and protein expression of NS were detected in 31 mucosal atypical hyperplasia specimens, 62 esophageal squamous cell carcinoma specimens and the matched normal esophageal mucosa samples by RT-PCR and immunohistochemistry method, respectively.

Results: The positive expression rate of NS protein in normal esophageal mucosa, atypical hyperplasia and esophageal squamous cell carcinoma was 17.

[Construction and identification of a eukaryotic expression vector for the small interfering RNA targeting nucleostemin gene].

Objective: To construct a eukaryotic expression vector for the small interfering RNA (siRNA) targeting nucleostemin (NS) gene.

Methods: The siRNA targeting NS gene was designed according to the sequence of NS mRNA available in GenBank. Three siRNA sequences were obtained, and the corresponding cDNAs were synthesized and inserted into plasmid pRNAT-U6.

[Detection of methylation of hMSH2 gene promoter region of esophageal cancer].

Objective: To detect methylation in promoter region of hMSH2 gene in esophageal cancer.

Methods: Specimens of cancer and normal tissues freshly removed from 32 cases of esophageal cancer patients without previous radiotherapy, chemotherapy or other treatment were preserved at -80 degrees C within 30 min. Methylation specific PCR (MSP) was used to detect methylation of mismatch repair gene (MMR) hMSH2 in promoter region in esophageal cancer and normal esophageal tissues.

[Expression of hMSH2 gene in esophageal cancer].

Objective: To observe the expression of DNA mismatch repair gene hMSH2 mRNA in esophageal cancer tissues.

Methods: This study included 32 esophageal cancer patients who received no previous radiotherapy, chemotherapy or other treatments. Within 30 min following surgical removal of the tumor tissues, specimens of the tumor, the tissue adjacent to the tumor and normal tissue at the esophageal stump (1 cmx1 cmx1 cm in size for each specimen) were obtained for examining hMSH2 expression with hMSH2 ISH detection kit.