[The effect of substances produced by a strain of Streptococcus sp. Thom-1606 on phagocytic activity].

Among the substances produced by Streptococcus sp. strain TOM-1606 two fractions having the opposite influence on the phagocytic activity of peritoneal macrophages of white mice were detected. The fraction corresponding to peptides with a molecular weight not exceeding 10 kD activated natural phagocytosis.

[A comparative study of 2 substances produced by the Streptococcus sp. Thom-1606 strain].

Among the substances secreted by Streptococcus sp. Thom-1606 [correction of TOM-1606], two substances exhibiting opposite biological action have been detected. Their antigenic structure, as indicated by the data of immunoprecipitation in agar, are not identical.

[Blood hydrolysates for microbiological culture media].

Blood hydrolysates contain two times less nucleic acids than meat hydrolysates, the levels of vitamins B2, B3, B6, and B9 are lower in them either. The authors suggest that this is the cause of a poorer growth of microorganisms in culture media with blood hydrolysates, repeatedly reported in literature. They have demonstrated that addition of nucleic acids and vitamins to blood hydrolysates improves the microorganism growth.

[The properties of the antibacterial preparation tomicide and the prospects of its use for treating staphylodermas].

The authors review the literature data and analyze their own findings on the properties of a bacteriocin-like drug tomicide, a filtrate of Streptococcus sp. Thom-1606 culture fluid. Tomicide is characterized by antibacterial action on the cocci group bacteria and activates the macroorganism phagocytic reaction.

[Use of a protective solution in the inoculation of bacterial cultures].

The influence of the composition of solutions used for growing the bacterial cultures on the survival of microorganisms has been studied. The use of a protective solution, consisting of sodium chloride, orthophosphates, magnium sulfate, and gelatin, for suspensions of bacterial cultures has provided a higher isolation rate of enteric microorganisms, vs. cultivation with 0.

[Development of Escherichia diagnostic O-test systems].

The principles of the rational development of new diagnostic preparations, Escherichia agglutinating O-test systems consisting of polyvalent reagents (reapols), were worked out, and two O-test systems, "small" and "large", were made up on the basis of these reagents (for the identification of 22 and 151 Escherichia serovars, respectively). The agglutinogenicity of Escherichia O-antigens was studied and found to be poorly pronounced in 40% of cases. The practical scheme for the identification of Escherichia O-antigens (key to the O-test system) has been made; the scheme permitted the determination of 84.

[Use of substances stimulating enterobacteria growth in obtaining microbial adsorbents].

The combination of substances, capable of stimulating the growth of enterobacteria and consisting of reducing agents (sodium thiosulfate and sodium sulfate), the vitamin preparation of yeast extract, polyatomic alcohol (glycerol) and ion exchange resin AB-17-8, is proposed. The addition of this combination of substances to culture media based on clots of animal blood has made it possible to achieve more intensive growth of Escherichia and Proteus than on classical meat culture media.

[Pseudomonas aeruginosa exotoxin].

Different P. aeruginosa strains have been found to differ in exotoxin synthesis. The strain isolated at the Mechnikov Research Institute for Vaccines and Sera (Moscow) and newly isolated cultures obtained from patients with the severe course of the infectious process have been found to possess the highest toxigenic activity and to synthesize exotoxins with the most complete set of pathogenically important antigens.

[Cultivation of Bordetella pertussis in liquid semisynthetic nutrient media with ion-exchange resins].

The possibility of optimizing the processes of B. pertussis cultivation carried out in liquid semisynthetic culture media with strong-base anion-exchange resins is shown. The use of strong-base ionites enhances the reproducibility of cultivation processes, increases the activity of microbial populations and the concentration of microorganisms.

[Highly specific agglutinating O- and OK-immunoglobulins for identification of Escherichia in the agglutination test on glass].

Escherichia agglutinating O- and OK-immunoglobulins of class G were obtained by the method of ion exchange chromatography; in studying with live and heated O- and K- cultures of the test strains from the International escherichia collection these immunoglobulins proved to be diagnostically highly specific and useful for the identification of escherichia strains in the express agglutination reaction on glass.

[Study of the fractional makeup of diagnostic nutrient media for isolating Vibrio cholerae].

A study was made of the fractional composition of peptones used for preparation of solid nutrient media. It was shown that the best growth of the vibrio on these media was obtained with the use of highly split peptones. Examination of fractional composition of peptone by gel-filtration and fractionation with various precipitants is recommended for rational working out of nutrient media for cultivation of cholera vibrio.