Comparison of the amniotic fluid and fetal urine peptidome for biomarker discovery in renal developmental disease.

Production of amniotic fluid (AF) is view as predominately driven by excretion of fetal urine (FU). However, the origin of AF peptides, often considered as potential biomarkers of developmental diseases, has never been investigated. Here, we evaluated the FU origin of AF peptides and if the AF peptide content can be used as a surrogate of FU.

Human urinary peptide database for multiple disease biomarker discovery.

Purpose: Human urine is an ideal candidate for use in clinical diagnostics. It is easily available, as untrained personnel can collect it. It correlates well with the pathophysiology of a number of diseases, making it a useful source for clinical proteomics.

Naturally occurring human urinary peptides for use in diagnosis of chronic kidney disease.

Because of its availability, ease of collection, and correlation with physiology and pathology, urine is an attractive source for clinical proteomics/peptidomics. However, the lack of comparable data sets from large cohorts has greatly hindered the development of clinical proteomics. Here, we report the establishment of a reproducible, high resolution method for peptidome analysis of naturally occurring human urinary peptides and proteins, ranging from 800 to 17,000 Da, using samples from 3,600 individuals analyzed by capillary electrophoresis coupled to MS.

CE-MS analysis of the human urinary proteome for biomarker discovery and disease diagnostics.

Owing to its availability, ease of collection, and correlation with pathophysiology of diseases, urine is an attractive source for clinical proteomics. However, many proteomic studies have had only limited clinical impact, due to factors such as modest numbers of subjects, absence of disease controls, small numbers of defined biomarkers, and diversity of analytical platforms. Therefore, it is difficult to merge biomarkers from different studies into a broadly applicable human urinary proteome database.

Proteomic patterns established with capillary electrophoresis and mass spectrometry for diagnostic purposes.

Background: Proteomics applied in large scale may provide a useful diagnostic tool.

Methods: We developed an online combination of capillary electrophoresis with mass spectrometry, allowing fast and sensitive evaluation of polypeptides found in body fluids. Utilizing this technology, polypeptide patterns from urine are established within 45 minutes.

Determination of peptides and proteins in human urine with capillary electrophoresis-mass spectrometry, a suitable tool for the establishment of new diagnostic markers.

The on-line coupling of capillary electrophoresis (CE) with electrospray-time-of-flight mass spectrometry (MS) has been used to obtain patterns of peptides and proteins present in the urine of healthy human individuals. This led to the establishment of a "normal urine polypeptide pattern", consisting of 247 polypeptides, each of which was found in more than 50% of healthy individuals. Applying CE-MS to the analysis of urine of patients with kidney disease revealed differences in polypeptide pattern.

Capillary electrophoresis coupled to mass spectrometry to establish polypeptide patterns in dialysis fluids.

Combination of capillary electrophoresis with mass spectrometry (CE-MS) allows generation of polypeptide patterns of body fluids. In a single CE-MS (45 min) run more than 600 polypeptides were analyzed in hemodialysis fluids obtained with different membranes (high-flux/low-flux). Larger polypeptides (M(r) > 10 000) were almost exclusively present in high-flux dialysates only, while in low-flux dialysates additional small polypeptides were detected.

[Characteristics of the development of immunity in healthy children during the first year of life].

Studies of the levels of serum IgA, IgG, and IgM, of B and T lymphocyte function in 20 neonates of days 7-8 of life (funic blood) and their mothers, in children aged 1,3,6,9 and 12 months made it possible to define the characteristic features of the establishment of humoral and cellular immunity during the first year of the child's life. The children placed under observation were normal as regards their physical and psychomotor development and had no infectious or inflammatory diseases. The neonates were characterized by high activity of T lymphocytes, the lack of IgA and traces of IgM, and the high level of IgG in blood serum.

[Surgical treatment of congenital ureteral stenosis in children with double upper urinary tract].

Based upon the experience with the operative treatment of 178 children with congenital stenoses of ureters and double urinary tracts the authors consider the upper heminephroureterectomy to be the main operation in the stenosis of the terminal part of the extra ureter. In stenosis of the prepelvic part of the ureter many modifications of pyeloureteral anastomoses can be used.

[Tumors of the urinary bladder in children].

The clinical picture, diagnosis and surgical methods of treatment of tumors of the urinary bladder are described in 7 children aged from 6 months to 2,5 years. Malignant tumors were found in 5, benign in 2 children.