(Heteroarylmethyl)benzoic Acids as a New Class of Bacterial Cystathionine γ-Lyase Inhibitors: Synthesis, Biological Evaluation, and Molecular Modeling.

Antibiotic resistance is one of the most serious global health threats. Therefore, there is a need to develop antimicrobial agents with new mechanisms of action. Targeting of bacterial cystathionine γ-lyase (bCSE), an enzyme essential for bacterial survival, is a promising approach to overcome antibiotic resistance.

The Diversity of Parasitoids and Their Role in the Control of the Siberian Moth, (Lepidoptera: Lasiocampidae), a Major Coniferous Pest in Northern Asia.

The Siberian moth, Tschetv., 1908 (Lepidoptera: Lasiocampidae) is a conifer pest that causes unprecedented forest mortality in Northern Asia, leading to enormous ecological and economic losses. This is the first study summarizing data on the parasitoid diversity and parasitism of this pest over the last 118 years (1905-2022).

[Initial mental disorders in patients with psychotic of schizophrenia in adolescent].

Objective: Clarification of the phenomenology and dynamics of initial mental disorders in patients with psychotic pubertal forms of schizophrenia, identification of the features of these disorders depending on sex.

Material And Methods: The study material was adolescent patients with psychotic forms of schizophrenia. Forty-three people were examined (women 44.

[Depressive disorders in adolescents in outpatient psychiatric practice].

Objective: To clarify the clinical features of depressive states in adolescent boys and girls (taking into account age and sex) seeking help from primary care specialists in outpatient psychiatric care.

Material And Methods: The study included 158 patients (89 boys and 69 girls), aged 15-17, with depressive pathology of various genesis, observed in the adolescent office of one of the Moscow psychoneurological dispensary in 2015-2020. The main research methods were clinical-psychopathological, clinical-follow-up, statistical.

A New Cypovirus-1 Strain as a Promising Agent for Lepidopteran Pest Control.

Now more than ever researchers provide more and more evidence that it is necessary to develop an ecologically friendly approach to pest control. This is reflected in a sharp increase in the value of the biological insecticide market in recent decades. In our study, we found a virus strain belonging to the genus (Reoviridae); the strain was isolated from , possessing attractive features as a candidate for mass production of biological agents for lepidopteran-pest control.

USP7 Inhibitors in Cancer Immunotherapy: Current Status and Perspective.

Ubiquitin-specific protease 7 (USP7) regulates the stability of a plethora of intracellular proteins involved in the suppression of anti-tumor immune responses and its overexpression is associated with poor survival in many cancers. USP7 impairs the balance of the p53/MDM2 axis resulting in the proteasomal degradation of the p53 tumor suppressor, a process that can be reversed by small-molecule inhibitors of USP7. USP7 was shown to regulate the anti-tumor immune responses in several cases.

[The first clinical experience of using the new Senhance remote-controlled robotic system in general surgery in Russia].

Unlabelled: Until recently, clinical studies in the field of robotic-assisted surgery have been conducted in Russia only on the basis of the DaVinci system. The advent of a new robotic system separated the concept of «robotic surgery» from DaVinci. An increase in the number of alternative surgical robots will expand the possibilities of studying the functionality of these systems, differences in their operation, safety and efficiency for patients, and convenience for surgeons.

Roux-en-Y gastric bypass and parastomal hernia repair: case report of concurrent operation in comorbid patient.

Introduction: The treatment of parastomal hernias remains one of the most relevant issues in coloproctology and general surgery due to its high recurrence rate of 5 to 50%. An increase in the number of overweight people has led to the fact that at least 25% of patients with parastomal hernias are obese and have severe concurrent disorders.

Presentation Of Case: A 69-years old woman with 12 × 15 cm parastomal hernia, grade 3 obesity and type 2 diabetes mellitus underwent concurrent laparoscopic IPOM hernia repair and Roux-en-Y gastric bypass.

Comprehensive Functional Analysis of Ribosomal RNA Methyltransferases.

Ribosomal RNAs in all organisms are methylated. The functional role of the majority of modified nucleotides is unknown. We systematically questioned the influence of rRNA methylation in on a number of characteristics of bacterial cells with the help of a set of rRNA methyltransferase (MT) gene knockout strains from the Keio collection.

[On the problem of prevalence of psychotic disorders in adolescents].

Objective: To analyze indicators of the referral rate for highly specialized inpatient psychiatric care in adolescents with psychotic disorders.

Material And Methods: Clinical/dynamic and statistical methods were used to study the data of patients, aged 14-17 years, with psychotic mental disorders according to ICD-10, as well as the indicators of their hospitalization in psychiatric consulting and medical institution during the calendar year.

Results And Conclusion: The analysis of 2017 data shows that 6.

Case report of successful management of intraoperative tracheal rupture during thoracoscopic esophageal resection in patient with esophageal cancer.

Introduction: A rupture of the membranous part of trachea during thoracoscopic and transhiatal resection of esophagus is a rare complication that occurs in 0.4% of cases. This complication often requires thoracotomy and is associated with prolonged pulmonary ventilation, long-term pleural draining due to persistent air leakage and development of a tracheopleural fistula, prolonged hospitalization, and high risk of septic and secondary cardiorespiratory complications.

codes for a mitochondrial peptide linking respiration and lipid metabolism.

Genes coding for small peptides have been frequently misannotated as long noncoding RNA (lncRNA) genes. Here we have demonstrated that one such transcript is translated into a 56-amino-acid-long peptide conserved in chordates, corroborating the work published while this manuscript was under review. The Mtln peptide could be detected in mitochondria of mouse cell lines and tissues.

Laparoscopic-thoracoscopic esophageal resection in the treatment of giant epiphrenic esophageal diverticulum (Ivor Lewis operation): Case report.

Introduction: Complicated esophageal diverticulum is an extremely rare disease. The most widespread surgical tactic in such cases is drainage of the mediastinum and the position of a feeding gastrostomy or enterostomy. Our objective was to demonstrate the possibility of performing a one-step operation in case of giant esophageal diverticulum with necrotic diverticulitis and sepsis on the background of dilatation of the esophagus.

[Revascularization in Stable Ischemic Heart Disease: Whether to Change the Approach?].

Current international guidelines on stable ischemic heart disease (IHD) state that coronary revascularization is indicated in cases of: limiting ischemic symptoms while on optimal medical therapy (OMT); high risk coronary anatomy; high risk stress test result. At the same time, many results supporting these conclusions were obtained in relatively old studies, conducted when the approaches to medical therapy and revascularization techniques differed greatly from contemporary practice. Many of the conclusions have been based on data from observational studies and subanalyses of randomized clinical trials, therefore methodologically are not sufficiently reliable.

[Application of reporter strains for new antibiotic screening].

Screening for new antibiotics remains an important area of biology and medical science. Indispensable for this type of research is early identification of antibiotic mechanism of action. Preferentially, it should be studied quickly and cost-effectively, on the stage of primary screening.

[Common features of antibacterial compounds: an analysis of 104 compounds library].

Antibacterial compounds are one of the essential classes of clinically important drugs. High throughput screening allowed revealing potential antibiotics active towards any molecular target in bacterial cell. We used a library of 9820 organic compounds with highly diversified structures to screen for antibacterial activity.

[Posttranscriptional messenger RNA modifications in eukaryotes].

Genomewide mapping of posttranscriptional modification in eukaryotic RNA allowed to reveal tens of thousands modification sites. Among modified nucleotides of eukaryotic RNA 6-methyladenosine, 5-methylcytidine, pseudouridine, inosine, and others. Many modification sites are conserved, many are regulated.

N6-Methylated Adenosine in RNA: From Bacteria to Humans.

N6-methyladenosine (m(6)A) is ubiquitously present in the RNA of living organisms from Escherichia coli to humans. Methyltransferases that catalyze adenosine methylation are drastically different in specificity from modification of single residues in bacterial ribosomal or transfer RNA to modification of thousands of residues spread among eukaryotic mRNA. Interactions that are formed by m(6)A residues range from RNA-RNA tertiary contacts to RNA-protein recognition.

[CURRENT VIEWS OF DIAGNOSTICS AND STRATEGY OF THE TREATMENT OF STABLE ISCHEMIC HEART DISEASE].

Ischemic heart disease (IHD) is a leading cause of mortality worldwide and stable IHD is one of its most important clinical forms. Recent decades brought new data on algorithms of diagnostics, pharmacotherapy and myocardium revascularization in patients with stable IHD. They were summarised in American (2012) and European (2013) recommendations on the management of this condition.

A bacterial homolog YciH of eukaryotic translation initiation factor eIF1 regulates stress-related gene expression and is unlikely to be involved in translation initiation fidelity.

YciH is a bacterial protein, homologous to eukaryotic translation initiation factor eIF1. Preceding evidence obtained with the aid of in vitro translation initiation system suggested that it may play a role of a translation initiation factor, ensuring selection against suboptimal initiation complexes. Here we studied the effect of Escherichia coli yciH gene inactivation on translation of model mRNAs.

[Adolescent's refusal of school: a psychopathological aspect].

Objective: Despite the multiple studies on the school refusal phenomenon, clinical/psychopathological aspects of this problem need further studies. Authors performed a clinical/psychopathological analysis of the school refusal phenomenon in adolescents who are under supervision of a psychiatrist.

Material And Methods: It were examined 223 adolescent patients (169 males and 54 females) with different appearances of school refusal.

Posttranscriptional modification of messenger RNAs in eukaryotes.

Transcriptome-wide mapping of posttranscriptional modifications in eukaryotic RNA revealed tens of thousands of modification sites. Modified nucleotides include 6-methyladenosine, 5-methylcytidine, pseudouridine, inosine, etc. Many modification sites are conserved, and many are regulated.

[New mutations in low-density lipoprotein receptor gene in familial hypercholesterolemia patients from Petrozavodsk].

Using an automated fluorescent single-strand conformation polymorphism (SSCP) analysis of the entire coding region, promoter zone, and exon-intron junctions of the low-density lipoprotein (LDL) receptor gene, we examined 80 DNA samples of patients with familial hypercholesterolemia (FH) from Petrozavodsk. We revealed mutations that might cause FH in five probands, including FH-North Karelia (c.925-931del7) mutation and four previously unknown mutations.

Familial hypercholesterolemia mutations in Petrozavodsk: no similarity to St. Petersburg mutation spectrum.

Background: Familial hypercholesterolemia (FH) is a human monogenic disease induced by a variety of mutations with striking genetic diversity. Despite this variability recurrent mutations occur in each population studied, which allows both elucidating prevalent mutations and developing DNA diagnostic tools for the disease. Recent research of FH in St.

Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysis.

Chemical landscape of natural RNA species is decorated with the large number of modified nucleosides. Some of those could easily be detected by reverse transcription, while others permit only high-performance liquid chromatography or mass-spectrometry detection. Presence of m(6)A nucleoside at a particular position of long RNA molecule is challenging to observe.