Molecular Characterisation of Isolates by Whole-Genome Sequencing.

is the most common non-tuberculous mycobacteria, known to be causing pulmonary and extrapulmonary diseases in humans. Based on molecular methods, has been previously classified into seven different subtypes. Now, based on whole-genome sequence analysis, a new species designation was proposed, in which species was designated subtype 1 and is of pathogenic significance in both immunocompetent and immunocompromised patients.

Validation of bedaquiline drug-susceptibility testing by BACTEC MGIT 960 system for .

Background: Bedaquiline (BDQ) is a new antituberculosis (TB) drug effectively used for the treatment of multidrug-resistant and extensively drug-resistant TB. However, the reports on drug-susceptibility testing (DST) for BDQ are scarce. The study aimed to validate and standardize BDQ DST by BACTEC MGIT 960 system for Mycobacterium tuberculosis.

Optimization of the conventional minimum inhibitory concentration method for drug susceptibility testing of ethionamide.

Evaluation of newer methods and optimization of existing methods for the susceptibility testing of second-line drugs, especially ethionamide, are essential when treatment of multidrug-resistant tuberculosis (MDR-TB) is warranted. The ideal method must clearly demarcate sensitive from resistant strains. Hence, optimization of the conventional minimum inhibitory concentration (MIC) method was attempted using diluted inoculum.