Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae).

The histochemistry of glycoproteins (GP) in the mucous cells of the gills of the silverside Odontesthes bonariensis was identified with: (1) oxidizable vicinal diols; (2) sialic acid and some of their chain variants, carbon 7 ((7) C), carbon 8 ((8) C) or carbon 9 ((9) C); (3) sialic acid residues without O-acyl substitution and with O-acyl substitution at (7) C, (8) C or (9) C; (4) carboxyl groups and (5) sulphate groups. A battery of seven biotinylated lectins allowed GPs sugar residues to be distinguished. Mucous cells showed the presence of neutral, sulphated and sialylated GPs.

Pectoral fins of Micropogonias furnieri: a histochemical and ultrastructural study.

The myotomal fibres of the pectoral fins of white croaker (Micropogonias furnieri) have been studied using histochemical techniques and transmission electron microscopy (TEM). Succinic dehydrogenase (SDH) for mitochondria, periodic acid Schiff (PAS) for glycogen, Sudan Black for lipids and myosin-adenosintriphosphatase (mATPase) pre-incubated at alkaline and acid pHs were used to visualize the contraction velocity. Three zones were determined: superficial (SZ), medium (MZ) and deep (DZ).

The mucosa of the digestive tract in Micropogonias furnieri: a light and electron microscope approach.

The histomorphological aspects as well as the histochemical content and distribution of glycoproteins (GPs) in the mucosa of the digestive tract of the white croaker Micropogonias furnieri were studied. The buccopharyngeal cavity and the esophagous showed a squamous stratified epithelium with mucous cells. The stomach presented three portions: cardias, fundus and pylorus.

Glycoconjugates in the mucosa of the digestive tract of Cynoscion guatucupa: a histochemical study.

This study addresses the histomorphology, the histochemistry and the distribution of glycoconjugates (GCs) in the mucosa of the digestive tract of the weakfish Cynoscion guatucupa. The histological characterization of the buccopharyngeal cavity and the esophagus revealed that they are lined by a stratified epithelium where the mucosa is thrown into longitudinal folds. The stomach is lined with a simple columnar secretory epithelium with tubular glands.

Histology and histochemistry of the pharyngeal cavity and oesophagus of the silverside Odontesthes bonariensis (Cuvier and Valenciennes).

The histology of the pharyngeal cavity and oesophagus of the freshwater 'silverside'Odontesthes bonariensis (Cuvier and Valenciennes) and the characteristics of their mucous cells were investigated. The histological characterization of its digestive wall revealed that the mucosa is thrown with longitudinal folds. The epithelium covering the folds was stratified with abundant mucous cells and gustative corpuscles, which are lacking in the oesophagus.

Ultrastructure and histochemical study of glycoconjugates in the gills of the white croaker (Micropogonias furnieri).

The ultrastructure of the primary and secondary lamellae of gills was investigated in a marine teleost, the white croaker. The following cells were identified and briefly described: pavement cells, mucous cells, mitochondria-rich cells and rodlet cells. These cell types are present throughout the length of the lamellae.

Morphological and histochemical characterization of the mucosa of the digestive tract in Engraulis anchoita.

The histomorphological aspects as well as the histochemical content and distribution of glycoproteins (GPs) in the mucosa of the digestive tract of the anchovy Engraulis anchoita were studied. The buccopharyngeal cavity is lined by a squamous stratified epithelium with mucous superficial cells; the oesophagus shows two zones, cranial with a squamous stratified epithelium with mucous superficial cells and caudal with a columnar secretory epithelium. Finally, the stomach presents both the cranial and pyloric portion lined with a simple columnar epithelium.

Mucous cells in micropogonias furnieri gills: histochemistry and ultrastructure.

The characteristics of the mucous cells located in the gills of the fish Micropogonias furnieri were investigated. Using histochemical procedures that included methods for localization and characterization of glycoproteins (GPs), no differences were detected between the mucous cell contents of the primary and secondary lamellae. The GPs were identified with (a) oxidizable vecinal diols; (b) sialic acids and some of their chain variants, C7 or C9; (c) carboxyl groups and (d) sulphate groups.

Characterization of the swimming muscle of the anchovy Engraulis anchoita (Hubbs and Martini 1935).

The lateral muscle characteristics of individual female anchovies (Engraulis anchoita) were studied by histochemical and ultrastructural techniques. Red, pink and white fibres were discerned on the basis of the activity of the metabolic enzymes succinic dehydrogenase, phosphorylase and myofibrilar ATPase, and the usage of Sudan and periodic-acid Schiff staining techniques. Red fibres were located at the surface, white fibres deep inside and pink fibres in between.

Lateral musculature in the whitemouth croaker (Micropogonias furnieri): its characterization with respect to different gonadal conditions.

Histochemical and ultrastructural studies were performed on the lateral musculature from individual female whitemouth croaker, Micropogonias furnieri, at the anterior, medium and posterior regions. Based upon histochemical myosin-ATPase (m-ATPase) determination, diverse types of red, pink and white fibres were discerned. Red muscle had abundant mitochondria and stained intensely for aerobic enzymes, white muscle scarcely stained for the same enzymes and pink muscle responded in an intermediate manner.

Branchial carbonic anhydrase (CA) of gills of Chasmagnathus granulata (Crustacea Decapoda).

The occurrence, localization and response to environmental salinity of carbonic anhydrase (CA) activity were studied in all of the gills of the euryhaline crab Chasmagnathus granulata from Mar Chiquita coastal lagoon (Buenos Aires Province, Argentina). CA activity in all gills appeared to be dependent on salinity. The pattern of distribution of CA activity among gills was different upon transition of C.

Characterization of lateral musculature in the striped weakfish (Cynoscion striatus Cuvier).

Histochemical and ultrastructural studies were performed on lateral musculature from individual female weakfish (Cynoscion striatus Cuvier). Based upon SDH, PhR, Sudan and myosin-ATPase determinations, different kinds of red, pink and white fibres were discerned at the anterior, medium and posterior regions. Levels of glycolytic activity increased from red to white fibres, being intermediate in the pink ones.

Erythrocyte alkaline phosphatase in patients with myotonic muscle disorders.

The allosteric behaviour of the p-nitrophenyl-phosphatase (E.C.3.

Erythrocyte membrane-bound acetylcholinesterase in vitamin E deficient rabbits.

1. The specific activities of erythrocyte membrane-bound acetylcholinesterase (EC 3.1.

Effect of chronic administration of verapamil in Duchenne muscular dystrophy.

1. In DMD patients the effect of chronic treatment with verapamil was investigated in the p-nitrophenylphosphatase from erythrocytes, the CK and LDH in serum and the functional activity of the muscle. 2.

Allosteric transition of erythrocyte alkaline phosphatase from Duchenne muscular dystrophy (DMD) patients and Duchenne muscular dystrophy carriers (Homo sapiens).

1. The kinetic properties of the p-nitrophenylphosphatase (EC 3.1.

4-Nitrophenylalkaline phosphatase inhibition in muscular dystrophy.

The allosteric behaviour of 4-nitrophenylphosphatase from membrane erythrocytes was investigated in Duchenne muscular dystrophy (DMD) patients, in female carriers and in healthy controls. Cooperative type kinetics with a Hill coefficient of -2.19, -1.

Acid phosphatase activity in pre- and post-spawning hake (Merluccius hubbsi).

1. The specific activity of muscle acid phosphatase (E.C.

The distribution of sorbitol dehydrogenase in embryonic and adult chicken tissues.

Sorbitol dehydrogenase activity (SDH) has been determined in various organs of embryonic and adult chickens. SDH is present in 24-hour embryos, and its activity continues to rise during the next 48 hours. During embryonic development and after hatching, regional differences in SDH activity are demonstrable in the organs of the animal.

Sorbitol dehydrogenase in Anas platyrhynchos and Zenaida auriculata auriculata during development.

Sorbitol dehydrogenase (E.C.N.