Publications by authors named "Gokula K Ramachandran"
Background: We evaluated the relevance of PD-1CD8 T-cells in gastric cancer (GC) including prognostic significance, association with chemotherapy and immunotherapy sensitivity and correlations with the tumor microenvironment (TME).
Methods: Discovery cohort: GC samples were evaluated for AE1/3, CD8, PD-1, Ki-67 and Granzyme-B expression with fluorescence-based multiplex immunohistochemistry (mIHC). Validation cohorts: we analyzed bulk RNAseq GC datasets from TCGA, the "3G" chemotherapy trial and an immunotherapy phase 2 trial.
Background: Irritable bowel syndrome (IBS) is a prevalent gastrointestinal disease characterized by intermittent abdominal pain with altered bowel habits. Due to the condition's chronicity, patients suffer from poor quality of life, while the healthcare burden continues to grow. There is currently no reliable biomarker for the diagnosis of IBS, and the current approach depends on ruling-out organic diseases such as inflammatory bowel disease (IBD) and colorectal cancer by markers of inflammation like fecal calprotectin and C-reactive protein, or invasive procedures like a colonoscopy.
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- PLK1 inhibitors show promise in treating certain hard-to-treat cancers, but not all patients with PLK1 overexpression respond to these drugs due to the absence of reliable patient selection markers.
- Research found that cancer cells lacking the ARID1A tumor suppressor gene are particularly sensitive to PLK1 inhibition, not due to its usual role in cell cycle regulation, but linked to issues in their mitochondrial functions.
- The study reveals a novel role for PLK1 in supporting mitochondrial health, especially under stress, and proposes a method for identifying patients who may benefit from PLK1 inhibitors based on ARID1A protein levels.
Objective: To characterize the differences between the primary and metastatic melanoma cell lines grown in 2D cultures and 3D cultures.
Methods: Primary melanoma cells (WM115) and metastatic melanoma cells (WM266) extracted from a single donor was cultured in 2D as well as 3D cultures. These cells were characterized using proton NMR spectrometry, and the qualitative chemical shifts markers were identified and discussed.
Existing gastric cancer diagnosing methods were invasive, hence, a reliable non-invasive gastric cancer diagnosing method is needed. As a starting point, we used 1H NMR for identifying gastric cancer biomarkers using a panel of gastric cancer spheroids and normal gastric spheroids. We were able to identify 8 chemical shift biomarkers for gastric cancer spheroids.
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