Cisplatin-mediated DNA double-strand breaks in replicating but not in quiescent cells of the yeast Saccharomyces cerevisiae.

DNA double-strand breaks (DSBs) are formed during the processing of DNA interstrand crosslinks in replicating yeast and Chinese hamster cells exposed to DNA crosslinkers such as psoralen plus UVA or nitrogen mustard. They were also detected in human cells after treatment with photoactivated psoralen or mitomycin C. In contrast, no DSBs were observed after exposure of Chinese hamster cells to cisplatin, another crosslinking agent widely used for the therapy of various cancers, challenging a common role for DSBs in the processing of DNA interstrand crosslinks.