Detection of Cervical Lesions and Cancer in Air-Dried Cytologic Smears by Combined Analysis of mRNA and miRNA Expression Levels.

Cervical cancer screening is based on cytologic analysis and high-risk human papillomavirus (HR-HPV) testing, each having their drawbacks. Implementation of new biomarker-based methods may improve screening accuracy. Here, the levels of 25 microRNAs (miRNAs, miRs) and 12 mRNAs involved in cervical carcinogenesis in 327 air-dried Papanicolaou-stained cervical smears from patients with cervical precancerous lesions, cancer, or without the disease were estimated by real-time PCR.

[Peculiarities of barbituric acid derivatives acute poisoning clinical picture in patients of elderly and senile age and their therapy.].

In this article materials obtained during treatment of 61 patients with acute poisoning with phenobarbital, which is part of Corvalol and Valocordin, are presented. It has already been established that phenobarbital acute poisoning in elderly and senile patients is accompanied by more frequent development of central nervous system, respiratory system (pneumonia) and cardiovascular system complications, which cause more severe clinical course and risk of an adverse outcome of acute poisoning. This research has shown that for hypoxia correction during phenobarbital acute poisoning in elderly and senile patients it is advisable to include Reamberin in the treatment regimen, which has no adverse effects on central haemodynamic parameters and effectively reduces severity of metabolic disorders.

Detection of high-grade neoplasia in air-dried cervical PAP smears by a microRNA-based classifier.

Recent studies have shown that changes in the expression levels of certain microRNAs correlate with the degree of severity of cervical lesions. The aim of the present study was to develop a microRNA-based classifier for the detection of high-grade cervical intraepithelial neoplasia (CIN ≥2) in cytological samples from patients with different high-risk human papillomavirus (HR-HPV) viral loads. For this purpose, raw RT-qPCR data for 25 candidate microRNAs, U6 snRNA and human DNA in air-dried PAP smears from 174 women with different cervical cytological diagnoses, 144 of which were HR-HPV-positive [40 negative for intraepithelial lesion or malignancy (NILM), 34 low-grade squamous intraepithelial lesions (L-SIL), 57 high-grade squamous intraepithelial lesions (H-SIL), 43 invasive cancers], were statistically processed.

[Results of multicenter study of efficacy and safety of divaza in the treatment of the asthenic and mild to moderate cognitive disorders in elderly and senile subjects].

Aim: To study the efficacy and safety of divasa in elderly and senile subjects with asthenic and mild to moderate cognitive disorders.

Material And Methods: The study included 126 patients with clinically significant asthenia and mild to moderate cognitive disorders. Asthenia was assessed with MFI-20, cognitive disorders with MMSE, clock drawing test and verbal association test.

[The use of reamberin in combined intensive care of acute poisoning].

This review is focused on the substrate antihypoxant reamberin used in intensive therapy of acute poisoning with special reference to the mechanisms of its action consisting in the reduction of the severity of metabolic disorders manifested as a decrease of tissue hypoxia, recovery of antioxidant defense and suppression of the activity of lipid peroxidation and endotoxicosis. The antihyhypoxant activity of reamberin is apparent as the reduction of the hypoxic brain lesions in patients with acute poisoning which leads to a significant improvement in the clinical course of acute poisoning and decreases the number of adverse outcomes.

[[Features of Pharmacological Correction of Alcoholic Fatty Liver Disease in Patients with Acute Ethanol Intoxication].].

The aim of the study was to increase the efficiency of the treatment of acute ethanol intoxication in patients with alcoholic fatty liver disease. The article presents the results which received during the investigation and treatment of 166 patients with acute ethanol in- toxication on the background of alcoholic fatty liver disease Patients were assessed by the severity scale APACHE-Il. Were studied the dynamics of clinical, laboratory, biochemical parameters, the state of the antioxidant system activity and lipid peroxidation.

[The development of kit for hybridization extraction of DNA and RNA of agents of hemotransmissive infections from serum and blood plasma].

To decrease dependence of effectiveness of isolation of nucleic acids of composition and amount of applied sample a kit was developed for hybridization extraction of DNA HBV RNA HCV and RNA HIV from blood serum in two formats--using up to 250 mkl and up to 1 ml of sample. This kit, in complex with kits for detection using polymerase chain reaction technique in real-time, forms a test characterized by high analytical sensitivity i.e.

[Mechanism of neuroprotector action of metabolic antihypoxant reamberin in patients with toxicohypoxic encephalopathy].

Based on investigation of a group of patients with heavy forms of sharp poisoning by neurotropic substances, it is established that inclusion if antihypoxant reamberin in intensive treatment of toxicohypoxic encephalopathy leads to a decrease in the depth of central nervous system depression. This is manifested by reduced duration of patient stay in coma and decreased total duration of resuscitation treatment. Improvement of the clinical picture is accompanied by a decrease in lipid peroxidation activity, increase in the level of antioxidant enzymes, and decrease in the level of NR2A auto-antibodies.

[Using cytoflavin for correcting x-ray contrasting agent-induced nephropathy].

Experimental data on the influence of omnipak-350 introduction in a dose of 20 g/kg on the glutathione exchange processes and lipide peroxidation in kidneys, liver and erythrocytes of white outbred rats are presented. The possibility of using cytoflavin for correcting x-ray contrast agent-induced disorders in the natural cytoprotective system is shown. Probable mechanisms of the cytoprotective effect of the proposed pharmacological treatment are discussed.

[Cytoflavin in polychemotherapy of breast cancer].

The efficacy of cytoflavin as a supporting drug in the CAF chemotherapy of breast cancer was studied. It was estimated by the tolerability, the peripheric blood index dynamics, the patients general condition (Karnovsky Index) and the frequency of the side effects. For the immediate estimation of the polychemotherapy and cytoflavin effects on the cytoprotection natural system state, the dynamics of the glutathione metabolism and the lipid peroxidation in the erythrocytes was investigated.

[Cardioprotective effect of cytoflavine on a model of doxorubicin-induced cardiomyopathy].

Experimental data on the influence of repeated administration of doxorubicin in a dose of 2.24 mg/kg on the glutathione exchange in heart tissues and erythrocytes of white outbred rats are presented. It was shown that disorders in the natural cytoprotection system caused by the introduction of this cardiotropic agent, can be corrected using a combined preparation of cytoflavin.

[Experimental study on cytoflavin pharmacological correction of cyclophosphamide-induced toxic affections of the liver and kidneys].

The experimental data concerning the influence of repeated administration of cyclophosphamide in a total dose of 200 mg/kg on the glutathione metabolism and lipid peroxidation in the liver and kidneys of albino noninbred rats are presented. The possibility of the natural cytoprotection system correction by the use of cycloflavin was shown. The cytoprotection action mechanisms of the pharmacological agent are discussed.

Decontamination of polymerase chain reaction reagents using DEAE-cellulose.

Polymerase chain reaction (PCR) is widely used to detect specific DNA sequences for purposes of microbial identification, clinical diagnosis, and basic research. The most pernicious problem plaguing this technique is contamination of PCR reagents with previously amplified material. We propose a useful tool for PCR reagent purification from contaminating nucleic acid using DEAE-cellulose and present the analysis of this technique for both decontamination efficiency and an effect on the reagent activity.

Determination of DNA polymerase and nuclease activities of DNA-dependent polymerases using fluorescence detection under real-time conditions.

A new method is proposed for estimation of polymerase activities using fluorescence detection during isothermal reaction. The method allows simultaneous determination of DNA-dependent DNA polymerase and 5'-3'-exonuclease activities using amplifiers supplied with an optical module for fluorescence detection under real-time conditions. Different primer-template combinations used as polymerase substrates were compared.

[Domestication of cereal crops in the old world: in search of a new approach to solving old problem].

N.I. Vavilov's theory of the centres of origin of cultivated plants is still as a methodological base for studying processes of domestication.

Divergent non-LTR retrotransposon lineages from the genomes of scorpions (Arachnida: Scorpiones).

We screened across the taxonomic diversity of order Scorpiones (22 species belonging to 21 genera and 10 families) for the presence of seven different clades of non-LTR retrotransposons in their genomes using PCR with newly designed clade-specific consensus-degenerate hybrid oligonucleotide primers. Scorpion genomes were found to contain four known non-LTR retrotransposon clades: R1, I, Jockey, and CR1. In total, 35 fragments of reverse transcriptase genes of new elements from 22 scorpion species were obtained and analyzed for three clades, Jockey, I, and CR1.

[Glutathione metabolism in rat cardiac tissues in acute doxorubicin-induced intoxication].

Experimental data are presented on the influence of acute doxorubicin intoxication with 7.47 and 2.34 mg/kg body mass on glutathione metabolism in cardiac tissues of albino non-inbred rats.

[Drug correction of hypoxia aftereffects in patients with toxic coma caused by acute neurotropic poisoning].

Seventy-eight patients with acute neurotropic poisoning (soporifics, neuroleptics, antidepressants, ethanol and its surrogates, narcotics) were examined and treated. Study of the pathogenesis of hypoxic injuries in acute neurotropic poisoning demonstrated the efficiency of using Perfluorane, an infusion solution with oxygen transporting functions, and Cytoflavine, a substrate antihypoxant, for correction of the detected disorders.

[Correction of free radical processes by a succinic acid drug (reamberine) in intensive care of patients with acute neurotropic poisoning].

Sixty-six patients with acute poisonings with neurotropic toxins (soporiphics, neuroleptics, antidepressants, ethanol and its surrogates) were examined and treated. Clinical data and correction of disorders in free-radical processes in acute poisonings with these toxins indicate the efficiency of adding reamberine to combined therapy of this patient population.

[The glutathione-dependent antioxidant system in rats during acute intoxication by hydrazine].

Intensity of lipid peroxidation in the liver, kidney and brain tissues, and functioning of the glutathione system were studied in purebred rats after acute intoxication by 1.1-dimethyl hydrazine at the dose of 104.5 mg/kg of body mass (LD50) and phenyl hydrazine at the dose of 170 mg/kg of body mass (LD50).

[Method for measuring glutathione system parameters in human lymphocytes].

The levels of reduced glutathion, activity of glutathion peroxidase, and glutathion S-transferase were measured in human peripheral blood mononuclears. The adapted methods permit measurements of glutathion system parameters in immunocompetent cells and can be used in patients with different immunopathological processes.