SIGNIFICANCE OF miRNA-185-5P AND miRNA-424-5P AS PROGNOSTIC MARKERS IN PROGRESSION OF EARLY-STAGE ENDOMETRIAL CANCER.

Aim: To compare the expression of miRNA-185-5p and miRNA-424-5p in tumor cells and peripheral blood serum (PBS) of patients with endometrioid carcinoma of the endometrium (ECE) and to evaluate the significance of these biomarkers in cancer progression.

Materials And Methods: The study was conducted on the samples of peripheral blood serum (PBS) and tumor tissue of 58 patients with stage I ECE using clinical and morphological methods and real-time polymerase chain reaction.

Results: A significant increase in the levels of circulating and tumor-associated miRNA-424-5p was established in ECE patients with a history of recurrences compared to patients without recurrences.

The KRAS, ATR and CHEK1 expression levels in endometrial cancer are the risk factors predicting recurrence.

Endometrial cancer is the most prevalent gynecologic malignancy with a high risk of recurrence. Local recurrence occurs in 7-20% of patients with treated stage I cancer within 3 years after primary treatment. In this study, we found significantly elevated mRNA expression levels of the oncoprotein KRAS, along with two replicative stress markers, ATR and CHEK1, in samples of endometrial carcinomas of endometrium (ECE) from patients with relapse.

Ultra-Fast Mass Spectrometry in Plant Biochemistry: Response of Winter Wheat Proteomics to Pre-Sowing Treatment with Iron Compounds.

In recent years, ultrafast liquid chromatography/mass spectrometry methods have been extensively developed for the use in proteome profiling in biochemical studies. These methods are intended for express monitoring of cell response to biotic stimuli and elucidation of correlation of molecular changes with biological processes and phenotypical changes. New technologies, including the use of nanomaterials, are actively introduced to increase agricultural production.

ROLE OF STROMAL MICROENVIRONMENT IN THE FORMATION OF INVASIVE, ANGIOGENIC, AND METASTATIC POTENTIAL OF ENDOMETRIOID CARCINOMA OF ENDOMETRIUM.

Unlabelled: The aim of the study was to determine the association of indicators of the progression of endometrioid carcinoma of the endometrium (ECE) with the type of stromal microenvironment, the counts of CXCL12+ fibroblasts and CD163+ macrophages, and the expression of the chemokine CXCL12 and its receptor CXCR4 in tumor cells.

Materials And Methods: Histological preparations of ECE samples (n = 51) were analyzed. Expression of CXCL2 and CXCR4 antigens in tumor cells, the content of CXCL12+ fibroblasts and CD163+ macrophages, and the density of microvessels were determined by the immunohistochemical method.

VALIDATION OF A PANEL OF BIOMARKERS ASSOCIATED WITH AGGRESSIVE PHENOTYPE OF ENDOMETRIOID CARCINOMA OF ENDOMETRIUM.

Aim: To evaluate the prognostic significance of a panel of biomarkers for the identification of a highly malignant molecular subtype of endometrioid carcinoma of the endometrium (ECE).

Materials And Methods: The expression of a number of markers (CD24, CD44, E2F1, FOXP3, Her2/neu, p21WAF1/CIP1, p53, β-catenin, vimentin, Е-cadherin, с-Myc, cyclins D1 and Е1) was determined by the immunohistochemical method in the samples of resected tumors of 127 patients with ECE of I-II stage. The Kullback method and the PanelomiX web tool were used to assess the informativeness and identify the aggressive subtype of ECE.

Molecular phenotype of high-grade endometrioid carcinoma of the endometrium.

Background: Prognosis of the course of tumor progression is one of urgent problems of clinical oncology. A relevant specificity of endometrial cancer is its clinical polymorphism within the same histological type of the disease. The search for molecular-biological features associated with the aggressive phenotype of endometrioid carcinomas is indisputably urgent.

Tomato response to metal nanoparticles introduction into the nutrient medium.

This study is aimed to explore the capacity of metal nanoparticles (NPs) iron, zinc, copper and their combinations introduced in the Murashige-Skoog (MS) nutrient medium (NM) to affect the growth and development of tomato plants ( L.). NPs were prepared by a flow-levitation method.

Overexpression of the mitochondrial ribosomal protein S18-2 in the invasive breast carcinomas.

Background: Recent studies allow to consider the mitochondrial ribosomal protein S18-2 (MRPS18-2, S18-2) as a potential oncoprotein, which suggests the need for further characterization of its expression in tumors of different genesis including breast cancer (BC). The aim of the study was to analyze the expression of the S18-2 protein in BC of luminal A and basal subtypes.

Materials And Methods: Operational material of BC patients stage І-ІІ (luminal A subtype, n = 30, and basal subtype, n = 10) was studied with the use of morphological, immunohistochemical, statistical and bioinformatic methods.

New insights into the cellular responses to iron nanoparticles in Capsicum annuum.

In this study, the anatomical and ultrastructural responses of Capsicum annuum to iron nanoparticles (Fe NPs) were determined. The results showed that the bio-effects of Fe NPs on plants could be positive or negative, depending on the additive concentrations. Low concentrations of Fe NPs were found to promote plant growth.

Phenotypic features of endometrial tumors in patients with family history of cancer.

Aim: To determine the peculiarities of expression of a number of proteins-regulators of the cell cycle in endometrial cancer (EC) cells in patients with a family history of oncological pathologies.

Patients And Methods: 95 EC patients (stage І-ІІ) were included into the study. Clinical-genealogical analysis was performed.

COMPLEX HEAVY METAL SALTS' EFFECT ON GANGLION NUCLEI NEURONS MORPHOLOGICAL FUNCTIONS IN ADULT MALE RATS' CEREBELLAR CORTEX.

In order to analyze the dynamics of morphological and morphometric nuclear rearrangements of cortical cerebellar Purkinje cells under prolonged exposure (for 90 days) on the body of copper sulfate, zinc and iron experiment was conducted on 48 white adult male rats weighing 200-250g, aged 5 -8 months. We used anatomic, morphometric, statistical and common methods of micro anatomical research method. It was found that the combined effect of copper sulfate, zinc and iron on the body has nuclear device ganglion neurons in the cerebellar cortex sufficiently expressive toxicity, which affects the state of neurons.

[Comparative analysis of genetic variants of the HIV-1 circulating in the Irkutsk region in 1999 and 2012].

The pol and env genome regions of the HIV-1 genetic variants circulating in the irkutsk region of russia in 1999 and 2012 were compared. The results of this work showed the dominance of the HIV-1 subtype a IDU-A genetic variant (100%) in this region. No primary resistance mutations in the pol gene in the treatment-naive patients were found.

Analysis of the env gene variability of the IDU-A HIV-1 variant in the outbreak of the HIV infection epidemic in Perm region of Russia (1996-2011).

In the present work, a total of 132 HIV-1 env gene C2-V3-C3 sequences belonging to the IDU-A genetic variant were analyzed. The variants were obtained from the viruses circulating among IDUs and heterosexuals in the Perm region at different periods. It was shown that the rate of the divergence of the IDU-A HIV-1 viruses from a common ancestor increased 4.

[Predicting of postoperative pain level and morphine consumption by preoperative pressure pain assessment in patients before elective surgery].

Unlabelled: The aim of this study was to predict a postoperative pain severity and morphine consumption by preoperative pressure pain assessment.

Design: 321 patients scheduled for elective surgery (lumbar discectomy, lumbar spinal fusion, hysterectomy, thoracotomy and total hip replacement) in 2009-2013 were enrolled in retrospective study. Pre-operatively, the pain threshold (PTH) and tolerance (PT) in Newton (N) were measured using the pressure algometry.

HIV Type 1 Subtype A1 Dominates in Armenia.

Objective: There is scarce information about the molecular epidemiology of HIV-infection in Armenia (former USSR). The objective of this work was to estimate the distribution of HIV-1 subtypes in this country and get any information about HIV drug resistance in naÏve patients.

Design: A joint study involving 78 patients was carried out in Yerevan, Armenia and Moscow, Russia in 2009-2013.

Comprehensive analysis of intratumoral lymphocytes and FOXP3 expression in tumor cells of endometrial cancer.

Aim: To study the tumor microenvironment (CD4(+), CD8(+) and FOXP3(+) lymphocytes) and FOXP3 expression by tumor cells and correlation of studied parameters with clinical and morphological characteristics of endometrial adenocarcinomas.

Materials And Methods: Tumor samples from 40 patients (mean age 56.9 ± 2.

DNA repair deficiency in peripheral blood lymphocytes of endometrial cancer patients with a family history of cancer.

Background: Individual susceptibility to endogenous and/or exogenous DNA damage depends on DNA repair efficiency and can be evaluated using the comet assay with bleomycin as genotoxic agent. The aim of the study was to evaluate baseline and bleomycin-induced DNA damage and DNA repair capacity in peripheral blood lymphocytes (PBLs) of endometrial cancer (EC) patients considering a family history of cancer.

Methods: DNA damage was analyzed in PBLs of 45 EC patients compared to a control group of 10 healthy women, using the comet assay.

Risk assessment of cancer of the female reproductive system.

Aim: To create an information resource concerning multifactorial oncological diseases of the female reproductive system.

Materials And Methods: A comprehensive search of the literature in the PubMed and Ukrainian scientific sources published from 1995 to 2014 and the results of researches performed in R.E.

HIV-1 genetic variants in the Russian Far East.

A molecular analysis of HIV-1 subtypes and recombinants circulating in cities in the Russian Far East was performed. The study included samples from 201 outpatients from Vladivostok, Khabarovsk, and Blagoveshchensk. In most parts of Russia, patients are infected with HIV-1 subtype A, known as the IDU-A variant.

[The influence of physical-chemical characteristics of surface modified copper nanoparticles on E. coli cell population growth suppression and on electrostatic properties of their membranes].

The biological activity of copper nanoparticles, able to suppress growth of E. coli cells population under contact interactions, was explored. Three types of samples with oxide layers of various sizes, thickness and composition were used in experiments.

[Copper-induced change in the ESR signal of hemoglobin nitrosyl complexes in wound by the action of copper nanoparticles].

The results concerning changes in the ESR signal of hemoglobin nitrosyl complexes in wound tissues in the course of healing by the action of ointments with copper nanoparticles (patent N2460532, Russia) are presented. It is shown that the wound healing process modified by the influence of copper nanoparticles demonstrates the increase in the ESR signal amplitude for :hemoglobin nitrosyl complexes as compared with controls (the ointment base without nanoparticles). Planimetric measurements of wound area through reparation course indicate an active process of wound healing for injuries treated with copper nanoparticles in the ointment, resulting in lessening half-reparation time up to 5.

[Copper nanoparticles as modulators of apoptosis and structural changes in some organs].

The effect of repeated intramuscular injection into the organism of copper nanoparticles (CNP) with the diameter of 103 nm on the index of cell readiness to apoptosis and the structure of liver, spleen, kidney, as well as sensomotor cerebral cortex, was studied in 78 male Wistar rats. CNP were injected once per week for 12 weeks. The organs were studied using histological, immunohistochemical and morphometric methods.

Influence of Cu10x copper nanoparticles intramuscular injection on mineral composition of rat spleen.

Spleen mineral composition was studied for 25 elements using atomic emission and mass spectrometry (AES-ISP and MS-ISP) for the first time by one- and manifold intramuscular injection of Cu10x copper nanoparticles. This was done, because the spleen is considered to be the organ most amenable of immunological defense and sensitive to exposure. The research is new and based on the necessity to check the accumulation of elements, which can result in toxic effects, and also the character of the influence of nanoparticles on the change of the natural fluctuation rhythm, as well as the interrelation between them.

[Wound healing potential of chitosan and its N-sulfosuccinoyl derivatives].

Comparative study of chitosan wound healing properties and its synthesized derivatives in MC-100 gel was carried out using the model of experimental full thickness skin wounds. It was determined that N-sulfosuccinoyl chitosan derivatives added into the gel in a concentration of 0.05% possess the higher wound healing activity in comparison with other chitosan derivatives and decrease the half-healing period of wounds 2-3 times in comparison with the control.

[Mechanism of the toxic action of copper nanoparticles on Escherichia coli bacteria].

Some features of contact interaction of Escherichia coli with Cu nanoparticles 75-100 nm in diameter have been studied. The analysis of variations in the amplitude of fluorescence of the negatively charged probe 1-anilinonaphthalene-8-sulfonate (ANS) in Gouy-Chapmen coordinates indicates an increase (in the absolute value) in the electrostatic potential of E. coli external membrane during the contact with Cu nanoparticles.