Photodynamic therapy with 5-aminolevulinic acid and diamino acid derivatives of protoporphyrin IX reduces papillomas in mice without eliminating transformation into squamous cell carcinoma of the skin.

Photodynamic therapy (PDT) is used to treat malignant and nonmalignant diseases. It is also used for cosmetological skin treatment. PDT is generally considered to have a low risk of carcinogenicity.

Diamino acid derivatives of PpIX as potential photosensitizers for photodynamic therapy of squamous cell carcinoma and prostate cancer: in vitro studies.

Photodynamic therapy (PDT) is an alternative treatment modality involving light activated drugs, called photosensitizers (PSs), to treat cancer and non-cancerous conditions. The search for new compounds which might become effective PSs is the major direction for PDT development. In the present work we have studied the dark toxicity, intracellular localization and photodynamic properties of four potential, water soluble, second generation PSs--PP(Arg)(2), PP(Ser)(2)Arg(2), PP(Ala)(2)Arg(2), PP(Phe)(2)Arg(2), all diamino acid derivatives of protoporphyrin IX.

Immunotherapy: a way to improve the therapeutic outcome of photodynamic therapy?

Photodynamic therapy (PDT) is a treatment for cancer and non-cancerous lesions involving light and a sensitizing drug, a so-called photosensitizer. Photosensitizers for PDT usually accumulate in tumour tissues with some selectivity. Thus, malignant and abnormal cells can be destroyed by PDT which acts by producing singlet oxygen and possible other reactive oxygen species.

[An immunoblotting as a method for the diagnosis of typhoid fever].

The patients' sera had been referred to the National Salmonella Centre for routine Widal serology. Sera were predominately from patients suspected of having been infected with Salmonella Typhi, but also included one serum from patient with typhoid fever who was culture positive for Salmonella Typhi. The immunoblotting procedure using Salmonella Typhi somatic (O=9,12 LPS) and flagellar (H=d) antigens was used for preliminary testing of selected patients sera previously evaluated by Widal agglutination assay as containing different levels of antibodies against O and/or H antigens of Salmonella Typhi.

Structure and heterogeneity of the O-antigen chain of Salmonella Agona lipopolysaccharide.

Lipopolysaccharide of Salmonella Agona smooth-type cells was obtained from bacteria by a hot phenol-water extraction procedure. Mild acid hydrolysis of lipopolysaccharide, followed by gel filtration, yielded the pure O-polysaccharide. Abequose, rhamnose, mannose, galactose and glucose in the molar ratio 0.

Influence of diamino acid derivatives of protoporphyrin IX on mouse immunological system: preliminary results.

Immunological response related to photodynamic therapy (PDT) is one of the basic elements that influence on the efficiency of this cancer treatment method. Diamino acid derivatives of protoporphyrin IX are promising photosensitizing agents that are intended to be the components of new anti-tumor drug. The influence of three derivatives - PP(Ser)(2)Arg(2), PP(Ala)(2)Arg(2), PP(Phe)(2)Arg(2) and a mixture of these compounds called Sensyphyrine on mouse immunological system was investigated where animals were exposed and unexposed to the laser irradiation.

Characterization of the anti-DnaJ monoclonal antibodies and their use to compare immunological properties of DnaJ and its human homologue HDJ-1.

Escherichia coli DnaJ (Hsp40) is suspected to participate in rheumatoid arthritis (RA) pathogenesis in humans by an autoimmune process. In this work a set of 6 anti-DnaJ monoclonal antibodies (mAbs) was raised and localization of the epitopes recognized by the mAbs was investigated. Western blotting and enzyme-linked immunosorbent assay (ELISA) experiments showed that the mAbs efficiently bound only native antigen.

Hsp60 specific antibodies in egg yolks from laying hens naturally infected with Salmonella enterica subspecies enterica serovar Enteritidis.

Heat shock protein (Hsp) 60 of Salmonella appears to be involved in pathogenesis of infectious processes and host immune responses. Eggs of laying hens from two Salmonella Enteritidis naturally infected flocks (I--acute outbreak of infection; II--occasional bacteria excretion) and one control flock (III) were tested for the presence of yolk antibodies (IgY) against Hsp60 by applying enzyme-linked immunosorbent assay (ELISA). The levels of specific immunoglobulins were related to those against lipopolysaccharide (LPS) and flagellin.

Human heat shock protein 60 (409-424) fragment is recognized by serum antibodies of patients with acute coronary syndromes.

Acute coronary syndromes (ACS), including unstable angina (UA) and acute myocardial infarction (MI), are clinical manifestations of a progressive atherosclerotic process. Antibodies (Ab) to heat shock proteins (hsp) have been reported to be associated with atherosclerosis. Blood samples from 35 patients with ACS and 20 healthy volunteers were tested for Ab to human hsp60 by an enzyme-linked immunosorbent assay (ELISA).

[Bacteriophage types of Salmonella enteritidis occurring in Poland in 1986-1995].

The Lalko phages collection was used to phage type a total of 517 Salmonella Enteritidis strains isolated from food-poisoning outbreaks (312 strains) and other common sources (205 strains) in Poland, during the years 1986-1995. Above 99.0% of all strains tested were recognized as belonging to definitive phage type.

[Use of WARD et al's scheme for bacteriophage typing of Salmonella enteritidis strains isolated in Poland].

The typing phages set of Ward et al. was used to type a total of 517 strains of Salmonella Enteritidis isolated in Poland in 1986-1995. According to the Ward et al.

[Salmonella serovars determined in the National Salmonella Center between 1995 and 1997].

A total of 897 human and non-human (from animal, food, feed and other sources) isolates of Salmonella were serotyped in the National Salmonella Centre during the period 1995-1997. They had been referred to the Centre by several field laboratories all over the country. Serological and biochemical identification was performed according to the standard methods.

[Salmonella serovars defined in Poland].

One hundred and sixty nine serovars of Salmonella, belonging to 28 different O groups, were defined in the National Salmonella Centre. All the serovars were isolated in Poland. Above 70% (121/169) of them belonged to five O groups--O:4 (31 serovars), O:7 (28 serovars), O:8 (27 serovars), O:3,10 (20 serovars) and O:9 (15 serovars).

Comparison of two Salmonella enteritidis phage typing schemes.

The comparison of two phage typing schemes for Salmonella enteritidis was performed. A total of 517 strains were phage-typed according to the schemes of Lalko [27] and Ward et al. [21].

Immunological and chemical studies of Salmonella haarlem somatic antigen epitopes. II. Serological investigations.

Lipopolysaccharide of Salmonella haarlem was hydrolyzed and the products separated. Native O-polysaccharide antigen was oxidised with sodium periodate followed by reduction with sodium borohydride. Native and chemically modified antigens were the subject of immunochemical studies.

Immunological and chemical studies of Salmonella haarlem somatic antigen epitopes. I. Structural studies of O-antigen.

Lipopolysaccharide (LPS) of Salmonella haarlem was hydrolyzed and the products separated. The structure of the O-specific polysaccharide (OPS) was found from sugar and methylation analyses. Rhamnose, mannose, galactose and tyvelose were detected and their linkage modes were established.

The epidemiological situation of Salmonella enteritidis in Poland.

The epidemiological situation connected with S. enteritidis in Poland, in the years 1961-1991, is described. During this period there were two increases in infections and food poisonings, which were of an epidemiological character.

Monoclonal antibodies against Vi antigen production and characterization.

As a result of fusion of in vitro immunized mouse splenocytes and myeloma Sp2/0 cells, stable hybridomas secreting monoclonal antibodies against Vi antigen were obtained. The monoclonal antibodies were of IgM class. Reactivity of monoclonal antibodies to Vi antigen of different origin was tested.

Distribution of Salmonella enteritidis phage-types in Poland in 1981-1990.

The occurrence of S. enteritidis phage types in Poland during the years 1981-1990 was examined. The strains were isolated from man, animals, and from food-stuffs.

Characteristics of lactose-fermenting Salmonella strains from Poland.

In this study 184 lactose-fermenting Salmonella strains, collected in the National Salmonella Centre from the northern and central parts of Ponad were examined. Epidemiological, serological and biochemical investigations were carried out. Apart from this, chemotherapeutic resistance and male-phage sensitivity were determined.

The action of the Yersinia pestis phage on the plague envelope antigen and the human erythrocytes.

The activity of the Yersinia pestis phage on the plague envelope antigen has been described. The purified envelope antigen was shown to possess the receptor properties. The phage caused changes of viscosity and hemagglutination properties of the antigen.