Experimental modeling of viral diseases of the locomotor system.

Dissemination of infectious inflammation was studied in experimental influenza and acute and chronic herpesvirus infections. The possibility of articular involvement into the infectious process was evaluated. Pathomorphological signs of changes in the articular tissue confirmed the effects of these viruses on the locomotor pathology.

[Neuropathogenecity of Coxsackie B virus strains isolated from children with clinical manifestations of aseptic meningitis in different years].

The neuropathogenicity of Coxsackie B3, B1, and B5 virus strains was studied in experimentally infected suckling BALB/c mice and in contact animals from the same nest and litter infected due to the natural virus spread. The similar neurological disorders were found in both groups of animals. The data on pathological and morphological changes in the medulla oblongata as dystrophic and necrotic changes in the neurons with their lysis and deletion, brain tissue edema, and hemodynamic changes confirmed the neutrotropicity of the strains under study.

[Molecular and epidemiological characteristics of tick-borne encephalitis virus in the Sverdlovsk Region on the basis of genotype-specific RT-PCR].

Studies and complete awareness of the regional and epidemiological properties of tick-borne encephalitis virus (TBEV) allow one to improve methods for preventing, diagnosing, and treating its severe neurological infection. The authors have developed reverse-transcriptase polymerase chain reaction (RT-PCR) systems for the detection of RNA of TBEV and for the determination of its genotype in the ticks and clinical materials. RT-PTC was shown to have a higher sensitivity and specificity than the practically used enzyme immunoassay system.

[Genetic variations of hepatitis C virus circulating in the Ural region].

A study of distributions of different genetic variations (subtypes) of hepatitis C virus (HCV), circulating in the territories of Yekaterinburg and Chelyabinsk among infected population categories of different social statuses and age, is reported in the paper. The predominance of 1b subtype was shown in the HCV-infected patients at the hemodialysis center (HDC) and at the pediatric oncohematology center (OHC), 83.3% and 84.

[The potentials for the comprehensive diagnosis of viral encephalitis in Sverdlovsk Province].

The suggested scheme of laboratory tests helped identify the etiology of viral encephalitides in 65.9% of cases. Diagnosis of tick-borne encephalitis was confirmed by laboratory findings in 93.

[Characteristics of a culture of embryonal human lung cells spontaneously contaminated with mouse cells].

The characteristics of human embryo lung culture spontaneously contaminated with mouse cells (HELCCM) are presented. Changes in the cytological and karyological characteristics of the cells were observed as compared with those of the initial human embryo lung (HEL) cell strain. The culture acquired the resistance to adenovirus type 7; at the same time RNA metabolism in HELCCM culture culture chronically infected with adenovirus practically did not differ from that of the control.

[Electron microscopic study of the usual and "minimal forms" of oncornaviruses in human embryonic lung cells cataminated with mouse cells].

Electron microscopic examinations of KLChKM (human embryo cells spontaneously contaminated with mouse cells) cell cultures revealed extracellular oncornaviruses of A and C types and intracisternal virions of A type which in their submicroscopical organization and morphogenesis were identical to oncornaviruses of mouse L cells. Also "minimal forms" (MiF) of oncornaviruses of A and C types the diameter of which was 30--70 nm were found. MiF of oncornaviruses were found to be formed in the process of budding through the cytoplasmic membrane or membranes of intracellular vacuoles.

[Isoenzyme characteristics of glucose-6-phosphage dehydrogenase and lactate dehydrogenase of continuous cells from the collection of the Sverdlovsk Research Institute of Virus Infections].

An analysis of isoenzymes of glucose-6-phosphage dehydrogenase (G-6-P-DH) and lactage dehydrogenase (LDH) of continuous cells from the collection of Sverdlovsk Research Institute of Virus Infections was carried out. Seventeen continous human and animal cell lines were examined by electrophoresis in a vertical block of 7% polyacrylamide gel followed by histochemical detection of the enzymes. The HEp-2, HEp-2 clone No.

[Isolation and study of biophysical properties of oncornaviruses type A and C minimal forms].

The method of differential centrifugation was used for concentration and purification of the minimal form of oncornaviruses type A and C. The structures showed heterogeneous distribution in sucrose density gradient and formed a peak in the zone of 1.135 g/ml.

[Characteristics of several indices of the mitotic regime of clone strains of HeLa cells infected with adenovirus type 7].

The effect of adenovirus type 7 on the mitotic activity, the level and qualitative characteristics of pathological mitoses was studied in mycoplasma-free and latently mycoplasma-infected cells of clone HeLa cell lines with different sensitivity to this virus. The level and the extent of changes of the mitotic regimen in adenovirus-infected cells depend upon their susceptibility to the virus and initial characteristics of the mitotic regimen of the culture which is somehow related to the presence or absence of mycoplasmal contaminants in the culture. Successful analysis of the virus effect on the mitotic regimen of one or another cell system requires the use of a culture with minimal pathology of the mitotic apparatus and careful tests for the lack of mycoplasmal contaminants.