The Evolution of Wisteria Vein Mosaic Virus: A Case Study Approach to Track the Emergence of New Potyvirus Threats.

Wisteria vein mosaic virus (WVMV, ), a virus belonging to the genus , is responsible for Wisteria vein mosaic disease (WMD), a severe disease that affects , a genus of garden plants acclaimed worldwide. Although probably originating in the Far East, WVMV infection was first reported in the US, and subsequently in numerous countries. Following the first molecular detection of an Italian isolate, WVMV Bari, its full-length genome was achieved using NGS barcoding technology.

Fast and Reliable Electronic Assay of a Xylella fastidiosa Single Bacterium in Infected Plants Sap.

Pathogens ultra-sensitive detection is vital for early diagnosis and provision of restraining actions and/or treatments. Among plant pathogens, Xylella fastidiosa is among the most threatening as it can infect hundreds of plant species worldwide with consequences on agriculture and the environment. An electrolyte-gated transistor is here demonstrated to detect X.

Phenotypic Characterization and Transformation Attempts Reveal Peculiar Traits of Subspecies Strain De Donno.

subsp. strain De Donno has been recently identified as the causal agent of a severe disease affecting olive trees in a wide area of the Apulia Region (Italy). While insights on the genetics and epidemiology of this virulent strain have been gained, its phenotypic and biological traits remained to be explored.

Differences in the Endophytic Microbiome of Olive Cultivars Infected by across Seasons.

The dynamics of infections in the context of the endophytic microbiome was studied in field-grown plants of the susceptible and resistant olive cultivars Kalamata and FS17. Whole metagenome shotgun sequencing (WMSS) coupled with 16S/ITS rRNA gene sequencing was carried out on the same trees at two different stages of the infections: In Spring 2017 when plants were almost symptomless and in Autumn 2018 when the trees of the susceptible cultivar clearly showed desiccations. The progression of the infections detected in both cultivars clearly unraveled that tends to occupy the whole ecological niche and suppresses the diversity of the endophytic microbiome.

Non-Lethal Effects of -Acetylcysteine on Strain De Donno Biofilm Formation and Detachment.

This study investigated in-vitro the non-lethal effects of -acetylcysteine (NAC) on subspecies strain De Donno (Xf-DD) biofilm. This strain was isolated from the olive trees affected by the olive quick decline syndrome in southern Italy. Xf-DD was first exposed to non-lethal concentrations of NAC from 0.

Ionomic Differences between Susceptible and Resistant Olive Cultivars Infected by in the Outbreak Area of Salento, Italy.

Olive quick decline syndrome (OQDS) is a devastating disease of olive trees in the Salento region, Italy. This disease is caused by the bacterium , which is widespread in the outbreak area; however, the "Leccino" variety of olives has proven to be resistant with fewer symptoms and lower bacterial populations than the "Ogliarola salentina" variety. We completed an empirical study to determine the mineral and trace element contents (viz; ionome) of leaves from infected trees comparing the two varieties, to develop hypotheses related to the resistance of Leccino trees to infection.

Draft Genome Sequence Resources of Three Strains (TOS4, TOS5, and TOS14) of Infecting Different Host Plants in the Newly Discovered Outbreak in Tuscany, Italy.

An outbreak of was discovered in late 2018 in northern Italy affecting several plant species. Multilocus sequence typing analyses detected the presence of strains clustering in subsp. and harboring a hitherto uncharacterized sequence type, ST87.