Integrating thermodynamics and mathematical modelling to investigate the stoichiometry and kinetics of sulphide oxidation-nitrate reduction with a special focus on partial autotrophic denitrification.

In the present study, the stoichiometry of the Sulphur Oxidizing-Nitrate Reducing (SO-NR) process, with a focus on Partial Autotrophic Denitrification (PAD), has been evaluated through a thermodynamic-based study whereas a model-based approach has been adopted to assess process kinetics. Experimental data on process performance and biomass yields were available from a previous work achieving efficient PAD, where a biomass yield of 0.113 gVSS/gS was estimated.

Successful sulphide-driven partial denitrification: Efficiency, stability and resilience in SRT-controlled conditions.

Partial denitrification is emerging as a valuable solution for NO supply in Anammox systems. When reduced sulphur compounds are used as electron donors, S-driven Partial Autotrophic Denitrification (PAD) can also be achieved, allowing for an integrated autotrophic nitrogen (N) and sulphur (S) removal from liquid and gaseous streams. The aim of the present work was to maximise NO reduction to NO coupled with complete HS oxidation, by the selective control of influent S/N ratio and sludge retention time (SRT).

Tannery Wastewater Recalcitrant Compounds Foster the Selection of Fungi in Non-Sterile Conditions: A Pilot Scale Long-Term Test.

This study demonstrated that a microbial community dominated by fungi can be selected and maintained in the long-term under non-sterile conditions, in a pilot-scale packed-bed reactor fed with tannery wastewater. During the start-up phase, the reactor, filled with 0.6 m of polyurethane foam cubes, was inoculated with a pure culture of and tannin, a recalcitrant compound widely used by tannery industry, was used as sole carbon source in the feeding.

Aerobic granular sludge treating anaerobically pretreated brewery wastewater at different loading rates.

In this study, three different aerobic granular sludge (AGS) reactors fed with anaerobically pre-treated brewery wastewater were studied. The AGS reactors were operated under different conditions including organic loading rates (OLR) between 0.8 and 4.

A novel universal primer pair for prokaryotes with improved performances for anammox containing communities.

Abundance profiling via 16S rRNA targeted next generation sequencing (NGS) is a common procedure to characterize mixtures of prokaryotic populations inhabiting an environment. Depending on the variable region/s addressed, different maps can be obtained due to their different information content. In this work, we focussed on wastewater microbial communities and we compared several recently developed universal primers that addressed regions V1-V3, V3-V4 and V4.

Role of bacterivorous organisms on fungal-based systems for natural tannin degradation.

Tannery wastewater presents high concentrations of organic load and pollutant recalcitrant molecules (e.g. tannins), which reduce the efficiency of biological treatment processes.

The core microbiome of sessile ciliate Stentor coeruleus is not shaped by the environment.

Microbiomes of multicellular organisms are one of the hottest topics in microbiology and physiology, while only few studies addressed bacterial communities associated with protists. Protists are widespread in all environments and can be colonized by plethora of different bacteria, including also human pathogens. The aim of this study was to characterize the prokaryotic community associated with the sessile ciliate Stentor coeruleus.

The role of cosubstrate and mixing on fungal biofilm efficiency in the removal of tannins.

Tannins are polyphenolic compounds produced by plants and they are used in industrial vegetable tanning of leather. Tannins represent one of the low biodegradability substances in tannery wastewaters with high recalcitrant soluble chemical oxygen demand, furthermore high concentration of tannins can inhibit biological treatment. In the present study, four novel rotating submerged packed bed reactors were inoculated with a selected fungal strain to reach a biological degradation of tannins in non-sterile conditions.

Hydrocarbonoclastic Ascomycetes to enhance co-composting of total petroleum hydrocarbon (TPH) contaminated dredged sediments and lignocellulosic matrices.

Four new Ascomycete fungi capable of degrading diesel oil were isolated from sediments of a river estuary mainly contaminated by shipyard fuels or diesel oil. The isolates were identified as species of Lambertella, Penicillium, Clonostachys, and Mucor. The fungal candidates degraded and adsorbed the diesel oil in suspension cultures.

Wastewater-Agar as a selection environment: A first step towards a fungal in-situ bioaugmentation strategy.

Viable and metabolically active fungi in toxic mixed liquors, treating landfill leachates and municipal wastewaters, were identified by culture depending methods. A selective culture medium consisting of wastewater and agar (WA) restrained fungi that could be randomly present (94% of the 51 taxa retrieved on WA were sample-specific), overcoming the problem of fast growing fungi or mycoparasite fungi. Moreover, WA allowed the isolation of fungi with a possible role in the degradation of pollutants typically present in the two wastewaters.

The microbial community in a moving bed biotrickling filter operated to remove hydrogen sulfide from gas streams.

The information available on the microbial communities responsible for pollutant degradation is increasingly accessible. Its use to optimize process design and operation is an important challenge in the field of effluent treatment research. Therefore, a prototype of a moving bed biotrickling filter (MBBTF) reactor was designed and, for the first time, operated at full-scale for the removal of sulfides desorbing from tannery industrial wastewater.

Tannery mixed liquors from an ecotoxicological and mycological point of view: Risks vs potential biodegradation application.

Fungi are known to be present in the activated sludge of wastewater treatment plants (WWTP). Their study should be at the base of an overall vision of the plant effectiveness and of effluents sanitary impact. Moreover, it could be fundamental for the implementation of successful bioaugmentation strategies aimed at the removal of recalcitrant or toxic compounds.

Effect of extended famine conditions on aerobic granular sludge stability in the treatment of brewery wastewater.

Results obtained from three aerobic granular sludge reactors treating brewery wastewater are presented. Reactors were operated for 60d days in each of the two periods under different cycle duration: (Period I) short 6h cycle, and (Period II) long 12h cycle. Organic loading rates (OLR) varying from 0.

Summer holidays as break-point in shaping a tannery sludge microbial community around a stable core microbiota.

Recently, several investigations focused on the discovery of a bacterial consortium shared among different wastewater treatment plants (WWTPs). Nevertheless, the definition of a core microbiota over time represents the necessary counterpart in order to unravel the dynamics of bacterial communities in these environments. Here we performed a monthly survey on the bacterial community of a consortial industrial plant.

Impacts of variable pH on stability and nutrient removal efficiency of aerobic granular sludge.

The impact of pH variation on aerobic granular sludge stability and performance was investigated. A 9-day alkaline (pH=9) and acidic (pH=6) pH shocks were imposed on mature granules with simultaneous chemical oxygen demand (COD), nitrogen and phosphorus removal. The imposed alkaline pH shock (pH 9) reduced nitrogen and phosphorus removal efficiency from 88% and 98% to 66% and 50%, respectively, with no further recovery.

Nitrifying biomass characterization and monitoring during bioaugmentation in a membrane bioreactor.

A membrane bioreactor (MBR), fed with domestic wastewater, was bioaugmented with nitrifying biomass selected in a side-stream MBR fed with a synthetic high nitrogen-loaded influent. Microbial communities evolution was monitored and comparatively analysed through an extensive bio-molecular investigation (16S rRNA gene library construction and terminal-restriction fragment length polymorphism techniques) followed by statistical analyses. As expected, a highly specialized nitrifying biomass was selected in the side-stream reactor fed with high-strength ammonia synthetic wastewater.

Modeling bioaugmentation with nitrifiers in membrane bioreactors.

Bioaugmentation with nitrifiers was studied using two pilot-scale membrane bioreactors, with the purpose of assessing the suitability of state-of-the-art activated sludge models (ASMs) in predicting the efficiency of bioaugmentation as a function of operating conditions. It was demonstrated that the temperature difference between seeding and seeded reactors (ΔT) affects bioaugmentation efficiency. Experimental data were accurately predicted when ΔT was within a range of up to 10 °C at the higher range, and when the temperature was significantly lower in the seeded reactor compared to the seeding one, standard ASMs overestimated the efficiency of bioaugmentation.

Biodegradation of naphthalenesulphonate polymers: the potential of a combined application of fungi and bacteria.

The potential of several fungi and their synergy with bacterial biomasses were evaluated as a solution for the removal of 2-naphthalensulphonic acid polymers (2-NSAPs) from petrochemical wastewater, characterized by a chemical oxygen demand (COD) greater than 9000 mg/L. The ability of fungi to grow on 2-NSAP mixtures was preliminarily investigated using a solid medium, and then the action of the selected strains, both in suspended and immobilized form, was evaluated in terms of degradation, depolymerization, sorption and an increase in biodegradability of 2-NSAP. Among the 25 fungi evaluated two, in particular, Bjerkandera adusta and Pleurotus ostreatus, have been found to significantly depolymerize 2-NSAP yielding to the corresponding monomer (2-naphthalenesulphonic acid, 2-NSA), which has been further degraded by a bacterial consortia selected in a wastewater treatment plant (WWTP).

Selection of denitrifying phosphorous accumulating organisms in IFAS systems: comparison of nitrite with nitrate as an electron acceptor.

Nitrite and nitrate were compared as electron acceptors to select for denitrifying phosphorous accumulating organisms (DPAO) in two integrated fixed film activated sludge (IFAS 1 and IFAS 2) systems operated as sequencing batch reactors. The bench-scale experiment lasted one year and synthetic wastewater was used as feed. During anoxic conditions 20mgNO3(-)-NL(-1) were dosed into IFAS-1 and 20mgNO2(-)-NL(-1) were dosed into IFAS-2.

Characterization and comparison of bacterial communities selected in conventional activated sludge and membrane bioreactor pilot plants: a focus on Nitrospira and Planctomycetes bacterial phyla.

A pilot-scale membrane bioreactor (MBR) and a conventional activated sludge system (CAS) were in parallel operated to investigate the impact of the separation technology on the structure and functionality of the selected microbial community. Microbial communities as well as nitrogen removal efficiency of the biomass were characterized. Kinetics and microbial community structure turned out to be duly correlated.

Effect of ammonia oxidizing bacteria (AOB) kinetics on bioaugmentation.

Bioaugmentation with ammonium oxidizing bacteria (AOB) was tested for 620d. A seeding reactor (R1), two seeded reactors (R2 at 21°C; R3 at 15°C) and an unseeded-control reactor (R4 at 21°C) were operated in parallel (2.4 View Article and Find Full Text PDF

Biomass accumulation modelling in a highly loaded biotrickling filter for hydrogen sulphide removal.

A pilot scale test on a biotrickling filter packed with polyurethane foam cubes was carried out for 110 d at high volumetric mass load (up to 280 g m(bed)(-3) h(-1)) with the aim of studying the accumulation of solids in the treatment of H(2)S. Removal rate up to 245 g m(bed)(-3) h(-1) was obtained; however, an accumulation of gypsum, elemental sulphur and, above all, inert biomass was identified as the cause of an increased pressure drop over the long term. A mathematical model was applied and calibrated with the experimental results to describe the accumulation of biomass.

Factors affecting the growth rates of ammonium and nitrite oxidizing bacteria.

The maximum specific growth rates of both ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) were investigated under varying aerobic solids retention time (SRT(a)) and in the presence/absence of anoxic (alternating) conditions. Two bench SBRs, reactor R1 and R2, were run in parallel for 150d. Reactor R1 was operated in aerobic conditions while R2 operated in alternating anoxic/aerobic conditions.

Modeling the decay of ammonium oxidizing bacteria.

A bench-scale sequencing batch reactor was used to study factors affecting the endogenous decay of the ammonium oxidizing biomass (AOB) in different operating conditions. AOB decay was very sensitive to oxygen concentration, and increased up to 0.4 d(-1) for oxygen concentration of 7 mg O(2) L(-1).

Monitoring biological sulphide oxidation processes using combined respirometric and titrimetric techniques.

The application of respirometric and titrimetric techniques to evaluate kinetic parameters and stoichiometry of the sulphide-oxidising biomass is a new promising approach for biotechnological sulphide oxidation process monitoring. It was possible to estimate the yield coefficients of each oxidation step of sulphide to elemental sulphur and to sulphate using respirometric tests, while evaluating the behaviour of the biomass in endogenous conditions. Furthermore, it was demonstrated how the combined application of titrimetric and respirometric techniques enabled the monitoring of sulphur and sulphate formation as a function of the environmental conditions.