Optimizing seedling production for the conservation of the threatened Dyckia rariflora (Bromeliaceae).

Objectives: In vitro seed propagation can enhance plant species growth and enable the rapid production of seedlings while preserving genetic variability. This study aimed to develop in vitro seed propagation and acclimatization protocols for Dyckia rariflora to support conservation efforts of this bromeliad endemic to ferruginous campos rupestres. Seed germination and plant growth were tested using MS (Murashige & Skoog) culture medium with varying salt concentrations, sucrose levels, and the presence or absence of polyvinylpyrrolidone (PVP).