Publications by authors named "Gisele Deblandre"
Article Synopsis
- ADHLSCs (adult-derived human liver stem/progenitor cells) have therapeutic potential for liver injury, but their delivery methods and long-term distribution are not well understood.
- This study used a triple fusion reporter system to track ADHLSCs after two different injection methods in mice, revealing significant differences in how these cells spread and retain in the liver.
- Findings showed that IS (intrasplenic) injections resulted in a more widespread distribution of ADHLSCs compared to IH (intrahepatic) injections, which were limited to the injection site, and both methods allowed monitoring of the cells for up to 4 weeks using bioluminescence imaging.
There is growing evidence that cell therapy constitutes a promising strategy for liver regenerative medicine. In the setting of hepatic cancer treatments, cell therapy could prove a useful therapeutic approach for managing the acute liver failure that occurs following extended hepatectomy. In this study, we examined the influence of delivering adult-derived human liver stem/progenitor cells (ADHLSCs) at two different early time points in an immunodeficient mouse model (Rag2-/-IL2Rγ-/-) that had undergone a 70% hepatectomy procedure.
View Article and Find Full Text PDFXenotransplantation of human cells in animal models is an essential tool for evaluation of safety and efficacy of cell-based products for therapeutic use. Sensitive and reproducible methods are needed to detect and quantify human cells engrafted into the host tissue either in the targeted organ or in undesired locations. We developed a robust quantitative polymerase chain reaction (qPCR) assay based on amplification of human AluYb8 repeats, to assess the number of human cells present in rat or mouse tissues after transplantation.
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