Applying cathodically polarised substrata to the restoration of a high value coral.

Larval settlement of the high value red coral, Corallium rubrum, was studied on three different CaCO(3) substrata, viz. lithogenic (marble), electro-accreted calcium carbonate in the presence and in the absence of cathodic polarisation. The last two substrata consisted of stainless steel plates galvanically coupled with Zn anodes.

Evidence of enzymatic catalysis of oxygen reduction on stainless steels under marine biofilm.

Cathodic current trends on stainless steel samples with different surface percentages covered by biofilm and potentiostatically polarized in natural seawater were studied under oxygen concentration changes, temperature increases, and additions of enzymic inhibitors to the solution. The results showed that on each surface fraction covered by biofilm the oxygen reduction kinetics resembled a reaction catalyzed by an immobilised enzyme with high oxygen affinity (apparent Michaelis-Menten dissociation constant close to K(O(2))(M)  ≈ 10 μM) and low activation energy (W ≈ 20 KJ mole(-1)). The proposed enzyme rapidly degraded when the temperature was increased above the ambient (half-life time of ∼1 day at 25°C, and of a few minutes at 50°C).

Exploiting a new electrochemical sensor for biofilm monitoring and water treatment optimization.

Bacterial biofilm development is a serious problem in many fields, and the existing biofilm monitoring sensors often turn out to be inadequate. In this perspective, a new sensor (ALVIM) has been developed, exploiting the natural marine and freshwater biofilms electrochemical activity, proportional to surface covering. The results presented in this work, obtained testing the ALVIM system both in laboratory and in an industrial environment, show that the sensor gives a fast and accurate response to biofilm growth, and that this response can be used to optimize cleaning treatments inside pipelines.

Electrochemical activity and bacterial diversity of natural marine biofilm in laboratory closed-systems.

Even if a widely shared mechanism actually does not exist, it is now generally accepted that, in aerobic conditions, marine electrochemically active biofilms (MEABs) induce faster oxygen reduction on stainless steel immersed in seawater. This phenomenon has been widely studied, but nearly all the experiments found in literature have been conducted in open-systems (i.e.