Application of the "echo-synch protocol" to advance pregnancy onset in ewe lambs at the first reproductive season.

Introduction: This study assessed the efficacy and economic impact of a reproductive protocol based on repeated ultrasound scanning (US) associated with the use of GnRH to advance pregnancy onset in ewe lambs.

Methods: Prepubertal ewe lambs ( = 133) were divided into three weight groups (High: HW  = 35; Medium: MW  = 65; Low: LW  = 33). Thereafter, animals were randomly allocated into two subgroups: GnRH, ewe lambs treated with GnRH analog and then exposed to rams; CTR, ewe lambs exposed to rams only.

Effect of Strategic Supplementation of Dietary By-Pass Linseed Oil on Fertility and Milk Quality in Sarda Ewes.

The aim of the present study was to assess whether the strategic supplementation of bypass LO can enhance reproductive indexes—fertility, lambing rate, and prolificacy—in dairy Sarda ewes at the end of lactation. To assess whether LO supplementation leads to the adsorptions of PUFAs and their subsequent utilization by the body tissues, milk composition and fatty acid content were analyzed. Forty-eight ewes were assigned to the following groups: the control group (CT; N = 24), fed with a control diet without LO; and the treatment group (LO; N = 24), fed with a diet supplemented with LO (10.

Does the timing of pasture allocation affect rumen and plasma metabolites and ghrelin, insulin and cortisol profile in dairy ewes?

A study was undertaken to assess the impact of the timing of grazing on rumen and plasma metabolites and some metabolic hormones in lactating dairy sheep allocated to an Italian ryegrass ( Lam) pasture in spring for 4 h/d. Twenty-four mid lactation Sarda ewes stratified for milk yield, body weight, and body condition score, were divided into four homogeneous groups randomly allocated to the treatments (2 replicate groups per treatment). Treatments were morning (AM, from 08:00 to 12:00) and afternoon pasture allocation (PM, from 15:30 to 19:30).

Sustainability in the Sardinian sheep sector: A systems perspective, from good practices to policy.

Three million sheep raised on 10 000 active farms operating in traditional and innovative farming systems in Sardinia, Italy, account for 13% of sheep milk production in the European Union (EU). Almost all of the milk delivered is processed to sheep cheese and is destined for world trade. The Sardinian dairy sheep sector also emits approximately 1600 kt CO eq/year, approximately 60% of regional livestock greenhouse gas (GHG), prompting the need for regional mitigation plans.

Plasma homoarginine concentrations in ewe's pregnancy and association with the number of fetuses.

A striking increase in homoarginine concentrations, about more than 100-fold that observed in humans, was recently reported during pregnancy in a nutritionally induced model of intra-uterine growth restriction in ewes. To determine whether this phenomenon is at least partially related to the nutritional regimen, estrus synchronization, or analytical method, thirty-four one-year-old primiparous, non-synchronized, and well-fed Sarda breed ewes were exposed to fertile rams allowing those who came into estrus to naturally mate. Plasma arginine, homoarginine, asymmetric dimethylarginine, symmetric dimethylarginine, mono methylarginine, and citrulline concentrations were measured in each sample using LC-MS/MS.

Pre- and Post-Slaughter Methodologies to Estimate Body Fat Reserves in Lactating Saanen Goats.

This work aimed to compare pre- and post-slaughter methodologies to estimate body fat reserves in dairy goats. Twenty-six lactating Saanen goats ranging from 43.6 to 69.

Can FT-Mid-Infrared Spectroscopy of Milk Samples Discriminate Different Dietary Regimens of Sheep Grazing With Restricted Access Time?

Milk obtained from sheep grazing natural pastures and some forage crops may be worth a plus value as compared to milk obtained from stall-fed sheep, due to their apparently higher content of beneficial fatty acids (FAs). Fourier transformed mid-infrared (FT-MIR) analysis of FA can help distinguish milk from different areas and diverse feeding systems. The objective was to discriminate milk from sheep and milk from dairy sheep rotationally grazing Italian ryegrass or berseem clover for 2, 4, or 6 h/day.

Towards a Sustainable Reproduction Management of Dairy Sheep: Glycerol-Based Formulations as Alternative to eCG in Milked Ewes Mated at the End of Anoestrus Period.

This study investigated whether the administration of equine chorionic gonadotrophin (eCG) in a protocol to induce and synchronize ovulations before mating could be replaced by the administration of glycerol-based formulations in milked ewes at the end of their seasonal anoestrus. Forty-eight late-lactation dairy ewes of the Sarda breed were synchronized using sponges impregnated with progestogen and then joined with fertile rams (day (D) 0, ram introduction). From D-4 to D-1, the ewes received by gavage either 100 mL of a glucogenic mixture (70% glycerol, 20% propylene glycol and 10% water; GLU group; = 24) or 100 mL of water (GON group; = 24) twice daily.

A Note on the Tracing of Herbage Contribution to Grazing Sheep Diet Using Milk and Feces Biomarkers.

Milk from grazing ruminants is usually rich in beneficial components for human health, but distinguishing milks sourced from grazing is difficult, and this hinders the valuing of the grazing benefit. This study aimed at evaluating the ability of milk biomarkers (1) to trace milks sourced from sheep submitted to different access times (ATs) to pasture and (2) to estimate sheep herbage dry matter intake (HDMI, g DM ewe d) and herbage percentage (HP, % DM) in sheep diet. Animal data derive from a published experiment in which six replicated groups of mid-lactation Sarda sheep had ATs of 2, 4, or 6 h d to a ryegrass pasture.

Administration of glycerol-based formulations in sheep results in similar ovulation rate to eCG but red blood cell indices may be affected.

Background: The objective of this study was to investigate the metabolic and osmotic effects of different doses of glycerol or a glycerol - propylene glycol mixture in Sarda sheep with the aim to identify those able to beneficially modify ewe's metabolic status without harmful changes in red blood cell (RBC) indices. Thereafter, the selected doses were tested for their effects on ewe's ovarian activity during an induced follicular phase and compared to the effects of a hormonal treatment with equine chorionic gonadotrophin (eCG).

Results: Glycerol was administered alone (G groups: 90% glycerol and 10% water; % v/v) or in combination with propylene glycol (M groups: 70% glycerol, 20% propylene glycol, 10% water; % v/v).

Circulating Concentrations of Key Regulators of Nitric Oxide Production in Undernourished Sheep Carrying Single and Multiple Fetuses.

The aim of this study was to investigate the blood concentrations of L-arginine, asymmetric dimethylarginine (ADMA), symmetric dimethylarginine (SDMA), and L-homoarginine, which are regulators of nitric oxide (NO) synthesis, in single, twin, and triplet pregnancies in ewes undergoing either a dietary energy restriction or receiving 100% of their energy requirements. From day 24 to 100 of pregnancy, the ewes were fed ryegrass hay and two different iso-proteic concentrates fulfilling either 100% of ewes' energy requirements (control group; = 30, 14 singleton pregnancies, 12 twin pregnancies, and 4 triplet pregnancies) or only 45% (feed-restricted group; = 29; 11 singleton pregnancies, 15 twin pregnancies, and 3 triplet pregnancies). Blood samples were collected monthly to measure, by capillary electrophoresis, the circulating concentrations of arginine, ADMA, homoarginine, SDMA, and of other amino acids not involved in NO synthesis to rule out possible direct effects of diet restriction on their concentrations.

Milk Urea Concentration in Dairy Sheep: Accounting for Dietary Energy Concentration.

In dairy sheep milk urea concentration (MUC) is highly and positively correlated with dietary crude protein (CP) content and, to a lesser extent, with protein intake. However, the effect of dietary energy and carbohydrate sources on MUC of lactating ewes is not clear. Thus, the objective of this study was to assess the effects of diets differing in energy concentration and carbohydrate sources on MUC values in lactating dairy ewes.

The assessment of supplementation requirements of grazing ruminants using nutrition models.

This paper was aimed to summarize known concepts needed to comprehend the intricate interface between the ruminant animal and the pasture when predicting animal performance, acknowledge current efforts in the mathematical modeling domain of grazing ruminants, and highlight current thinking and technologies that can guide the development of advanced mathematical modeling tools for grazing ruminants. The scientific knowledge of factors that affect intake of ruminants is broad and rich, and decision-support tools () for modeling energy expenditure and feed intake of grazing animals abound in the literature but the adequate predictability of forage intake is still lacking, remaining a major challenge that has been deceiving at times. Despite the mathematical advancements in translating experimental research of grazing ruminants into DST, numerous shortages have been identified in current models designed to predict intake of forages by grazing ruminants.

The influence of plant polyphenols on lipolysis and biohydrogenation in dried forages at different phenological stages: in vitro study.

Background: It is known that forage legumes show a higher transfer efficiency of polyunsaturated fatty acids (PUFA) to ruminant dairy products in comparison with grasses. Legumes are usually characterised by moderate levels of plant secondary metabolites, which can have an effect on lipolysis and biohydrogenation in the rumen. An in vitro study was carried out to compare two species with different plant phenol compositions, Vicia sativa (VS, common vetch, cv.

Fast cardiologist-administered midazolam for electrical cardioversion of atrial fibrillation.

Aim: To evaluate the safety and the feasibility of sedation administered by cardiologists with rapid intravenous bolus of midazolam followed by flumazenil infusion during transthoracic biphasic electrical cardioversion (TEC) for atrial fibrillation (AF).

Methods: Two hundred and sixty-five consecutive patients (119 females, mean age 67.4 +/- 8.

Bioremediation of olive oil mill wastewater and production of microbial biomass.

Olive oil mill wastewater (OMWW) was used as a substrate for the culture of a mixture of edible fungi in order to obtain a potentially useful microbial biomass and to induce a partial bioremediation of this fastidious waste. Before fermentation, the OMWW underwent an alkaline-oxidative treatment with the aim of decreasing the polyphenolic content which is the main cause of its toxicity. The fungal mixture grew fairly well in the treated OMWW and reached a maximum of biomass production within about 14 days of fermentation at room temperature.

Determination of poly(ethylene glycol)-binding to browse foliage, as an assay of tannin, by near-infrared reflectance spectroscopy.

Nutritionists are interested in functional assays of tannins that do not require time-consuming and expensive extraction, such as the (14)C-labeled poly(ethylene glycol) (PEG)-binding (PEG-b) assay. This paper reports the application of near-infrared (NIR) spectroscopy to determine the percentage of PEG binding, in place of the (14)C-labeled PEG-b assay of tannin, in Mediterranean woodland vegetation. Calibration was done with 53 samples from 14 species and was validated on 25 samples from 10 species.