Antibiotic resistance spread potential in urban wastewater effluents disinfected by UV/H2O2 process.

Urban wastewater treatment plants (UWTPs) are among the main hotspots of antibiotic resistance (AR) spread into the environment and the role of conventional and new disinfection processes as possible barrier to minimise the risk for AR transfer is presently under investigation. Accordingly, the aim of this work was to evaluate the effect of an advanced oxidation process (AOP) (specifically UV/H2O2) on AR transfer potential. UV/H2O2 disinfection experiments were carried out on real wastewater samples to evaluate the: i) inactivation of total coliforms, Escherichia coli and antibiotic resistant E.

β-lactams resistance gene quantification in an antibiotic resistant Escherichia coli water suspension treated by advanced oxidation with UV/HO.

Water is one of the most important habitats and route for the spread of antibiotic resistance (AR) in the environment and disinfection processes can be a potential barrier to minimise this risk. In this study the effect of UV/HO process on the potential of AR transfer was investigated through cultivation methods vs (polymerase chain reaction) PCR based methods. bla was selected as target antibiotic resistance gene (ARG) and was quantified by qPCR in the survived colonies and the whole suspension (total DNA).

Cross-Contamination of Residual Emerging Contaminants and Antibiotic Resistant Bacteria in Lettuce Crops and Soil Irrigated with Wastewater Treated by Sunlight/H2O2.

The sunlight/H2O2 process has recently been considered as a sustainable alternative option compared to other solar driven advanced oxidation processes (AOPs) in advanced treatment of municipal wastewater (WW) to be reused for crop irrigation. Accordingly, in this study sunlight/H2O2 was used as disinfection/oxidation treatment for urban WW treatment plant effluent in a compound parabolic collector photoreactor to assess subsequent cross-contamination of lettuce and soil by contaminants of emerging concern (CECs) (determined by QuEChERS extraction and LC-QqLIT-MS/MS analysis) and antibiotic resistant (AR) bacteria after irrigation with treated WW. Three CECs (carbamazepine (CBZ), flumequine (FLU), and thiabendazole (TBZ) at 100 μg L(-1)) and two AR bacterial strains (E.

Inactivation and regrowth of multidrug resistant bacteria in urban wastewater after disinfection by solar-driven and chlorination processes.

Solar disinfection and solar-driven advanced oxidation processes (AOPs) (namely H2O2/sunlight, TiO2/sunlight, H2O2/TiO2/sunlight, solar photo-Fenton) were evaluated in the inactivation of indigenous antibiotic-resistant bacteria (ARB) in real urban wastewater. A multidrug resistant (MDR) Escherichia coli strain isolated from the effluent of the biological process of an urban wastewater treatment plant was the target ARB. The higher inactivation rates (residual density under detection limit, 2 CFUm L(-1)) were achieved with H2O2/TiO2/sunlight (cumulative energy per unit of volume (QUV) in the range 3-5 kJ L(-1), depending on H2O2/TiO2 ratio) and H2O2/sunlight (QUV of 8 kJ L(-1)) processes.

Vancomycin resistant enterococci: from the hospital effluent to the urban wastewater treatment plant.

Vancomycin is an important antibiotic to treat serious nosocomial enterococci infections. Human activities, in particular those related with clinical practices performed in hospitals, can potentiate the transfer and selection of clinically-relevant resistant bacteria such as vancomycin resistant enterococci (VRE). Indeed, previous studies demonstrated the occurrence of VRE in urban wastewater treatment plants and related environments (e.