[In-hospital triage in 2022].

The in-hospital triage is the first step to every emergency department visit, and it is considered the main tool for matching medical and nursing resources to an exploding demand for care. In the current organization of care, triage is done by a triage nurse who can in certain situations request medical advice. However, given the increase in the number of visits to the emergency room, the heterogeneity of the demand of care and the perception of patients as to their needs, the role of a medical-nursing tandem is taking shape.

[Pulmonary. High tech in pulmonary medicine: GeneXpert, coils and bronchial thermoplasty].

We discuss here three recent applications of high technology in pulmonary medicine. GeneXpert has considerably improved the diagnosis of active tuberculosis. However, the test has also its limits so that clinical assessment, radiology, and sputum microscopy remain fundamental.

Efficient induction of CD8 T-associated immune protection by vaccination with mRNA transfected dendritic cells.

Dendritic cells are excellent targets for antigen-specific immune intervention. Here we attempted to introduce a CD8 T cell-dependent epitope into dendritic cells for presentation on major histocompatibility complex class I and induction of immunity. Murine bone-marrow-derived dendritic cells were subjected to electroporation with mRNA transcribed in vitro from a plasmid encoding lymphocytic choriomeningitis virus glycoprotein or enhanced green fluorescent protein under the control of a T7 promotor.