[Myocardial injuries induced by oxidative stress in rat--experimental morphopathological study].

Unlabelled: Oxidative stress is a phenomenon significantly involved in several clinical conditions such as ischemic heart disease and myocardial reperfusion.

Purpose: Creating an animal model of oxidative stress based on three methods: exertional (swimming), habitual (light exposure), and corticotropine injections.

Material And Methods: 40 Wistar adult rats, weighting between 210-315 g, were subjected to either none (control group), swimming, light exposure or corticotropine injections for six weeks, then the animals were sacrificed and a light and electronic microscopic examination of the myocardium and ascending aorta were performed.

[Ki-67 and p53 expressions versus differentiation degrees of colon adenocarcinoma].

Purpose: The colon carcinoma prognostic is evaluated with respect to four categories of factors established by College of American Pathologists Consensus Statement 1999, among which a series of molecular markers is also included. Our study aimed to develop semiquantitative analysis of the immunohistochemistry pattern for proliferation and apoptosis markers, as well as of their connections with the histopathology subtypes of colon adenocarcinoma.

Material And Methods: The group of study included 5 cases of well-differentiated adenocarcinoma, 5 cases of moderately differentiated adenocarcinoma and 5 cases of poor differentiated adenocarcinoma.

Ki-67, p53 and bcl-2 analysis in colonic versus rectal adenocarcinoma.

This paper develops a comparative study between similar subtypes of colonic and rectal adenocarcinoma, based on their immunohistochemical profiles for Ki-67, p53 and bcl-2 markers, in order to evaluate the prediction value for the investigated markers, according to the histologic subtype and location. Thirty cases of adenocarcinoma were investigated, 15 with colonic and 15 with rectal location. For both locations, the cases included five well-differentiated, five moderately differentiated and five low differentiated subtypes.

T-cell primary cutaneous lymphomas. A clinicopathological and immunohistochemical study.

Within the large framework of the lymphoproliferative diseases, the primary cutaneous lymphomas are distinct pathologic conditions, defined by particular morphologic, immunologic, genetic, and clinic criteria. The study aimed to create the first clinicopathological and immunohistochemical profile of primary cutaneous lymphoma for a Romanian region. We investigated a series of 16 cases (diagnosed during a 5-year period) in accordance with the general principles of primary cutaneous lymphoma management.

Immunohistochemical markers in the study of gallbladder premalignant lesions and cancer.

Aim: The aim of the study was to perform a comparative analysis of proliferative and apoptotic activity as well as of microvascular density (MVD) in metaplasia, dysplasia and gallbladder carcinoma.

Material And Method: There were investigated 10 cases of chronic cholecystitis associating premalignant lesions and 5 cases of gallbladder carcinoma developed on a chronic cholecystitis background. The immunohistochemical (IHC) staining was made using Ki-67, p53 and CD34 antibodies, and the streptavidin-biotin method.

Hyperplasia, metaplasia, dysplasia and neoplasia lesions in chronic cholecystitis - a morphologic study.

The aim of the study was to analyze the association between chronic cholecystitis, premalignant lesions and gallbladder cancer. The group consisted in 3901 cases of cholecystectomies, diagnosed as acute cholecystitis (250 cases - 6.4%), chronic cholecystitis (3619 cases - 92.

[The detection of dysplastic lesions of the gallbladder mucosa in polypoid cholelithiasis].

Aim: To investigate the hyperplastic and/or dysplastic lesions in the gallbladder mucosa.

Methods: We used the immunohistochemical Ardeleanu-Hsu-Bussolati and Gugliotta methods, with avidin and peroxide biotin, adapted in the laboratory of University Hospital "St. Spiridon" Iaşi.

Computerized morphometric discrimination between normal and tumoral cells in oral smears.

The oral exfoliative cytology allows a quick and fairly accurate assessment of suspicious lesions of the oral cavity. Within this context, our paper proposes a quantitative approach, focusing on the construction of a classifier for detecting the presence of the tumoral cells on oral smears. The design of the classifier relies on a detailed computerized analysis of the individual morphometric features exhibited by two large known populations of normal and tumoral cells, respectively; the digital image processing was performed in the Zeiss KS400 environment.

[Transcriptional and electron microscopic analysis of extracellular matrix proteoglycans in acute acetaminophen intoxication].

Unlabelled: Acetaminophen (APAP) OTC regimen is not compatible with kidney and liver toxic effects. In order to further understand the determinism of the repair process, we have studied the expression of some target extracellular matrix proteoglycan components in an animal model.

Materials And Methods: An acute APAP toxic dose (800 mg/bw) was administered to 45 mice aged 1 month.

[Pulmonary alveolar proteinosis in AIDS patients].

We present 4 patients with pulmonary alveolar proteinosis and AIDS which died in Infectious Disease Hospital Iaşi in the interval January 2002-March 2003. The pulmonary tissue from the necropsy exam was stained with H.E.

[Hyperthyroidism and coexistent thyroid carcinoma].

The association between thyrotoxicosis and thyroid cancer is a classical, rare and controversial entity. Within their series of eleven such cases the authors dissociate two distinct groups from clinical and pathological viewpoint. The first category is represented by one case of follicular thyroid cancer with clinically and biologically confirmed hyperthyroidism.

[Electron microscopy in oral squamous cell carcinoma of elderly patients].

Objectives: Squamous cell carcinoma is the most frequent tumor occurring in the oral cavity. The aim of the study is to point out the fine-structural characteristics of the tumor cells and of the adjacent stromal cells, trying to establish a correlation between these characteristics and the biological behavior of the tumor.

Material And Methods: We performed an electron microscopic investigation for tree elderly patients diagnosed with oral squamous cell carcinoma.

[Electron microscopy study regarding the etiology and the pathogenesis of acne vulgaris].

The etiology and the pathogenesis of acne vulgaris are not complete cleared up yet. This work presents the results of the electronomicroscopic examinations effected on lesions of acne vulgaris biopsied from 5 patients with different clinical forms of disease (including acne fulminans). In this study we had in view the investigation and description of the ultrastructural modifications that followed the intervention of the 4 factors incriminated in the etiology and pathogenesis of acne: sebaceous hypersecretion, hyperkeratosis pilosebaceous infundibulum, bacterial colonisation, perifollicular inflammation.