Differential regulation of normal and tumor alpha 1-fetoprotein genes in fetal hepatocyte x hepatoma hybrids.

Fetal rat hepatocytes and mouse hepatoma cells actively expressing alpha 1-fetoprotein (AFP) and albumin genes were fused with the use of Sendai virus, and the expression of normal (rat) and tumor (mouse) AFP and albumin genes was analyzed in hybrid clones. The tumor AFP gene and both albumin genes were active in 103 hybrids. Expression of the normal fetal rat AFP gene, however, was maintained in only 3 hybrids, and it was frequently lost or decreased selectively upon subcloning.

Enhancer and promoter elements directing activation and glucocorticoid repression of the alpha 1-fetoprotein gene in hepatocytes.

Mutations were introduced in 7 kilobases of 5'-flanking rat alpha 1-fetoprotein (AFP) genomic DNA, linked to the chloramphenicol acetyltransferase gene. AFP promoter activity and its repression by a glucocorticoid hormone were assessed by stable and transient expression assays. Stable transfection assays were more sensitive and accurate than transient expression assays in a Morris 7777 rat hepatoma recipient (Hepa7.

Natural killer cell regulation of implantation and early lung growth of H-ras-transformed 10T1/2 fibroblasts in mice.

We examined the relative role of the natural killer (NK) cell and H-ras gene in controlling metastasis formation using a novel assay for quantitating viable tumor cells entering and surviving in the lung for up to 13 days following i.v. tumor inoculation.

Marital enrichment: the contribution of sixteen process variables to the effectiveness of a program.

A marital enrichment program, based on Sager's contractual model of couple functioning, was analyzed to see which of its components were linked to good and/or improved marital adjustment, communication and problem-solving skills. Nineteen couples underwent the eight-week program, and were pre- and posttested on the Dyadic Adjustment Scale, the Marital Communication Inventory, and the Marital Interaction Coding System. A factor analysis of 16 process variables within the program confirmed the relationship between components of the program and the therapeutic goals based on Sager's model.

Oncodevelopmental and hormonal regulation of alpha 1-fetoprotein gene expression.

The main features of the oncodevelopmental biology of alpha 1-fetoprotein (AFP) are reviewed. Progress made in the molecular biology of AFP gene regulation is discussed and we present our recent data on the mechanisms of AFP suppression by glucocorticoid hormones. The relationship between AFP gene transcription and cell replication is examined, and it is suggested that the degree of methylation of the AFP gene (or of co-methylated regulatory DNA sequences) conditions its response to hormones.

Short- and long-term effects of a marital enrichment program upon couple functioning.

Short- and long-term effects of a new marital enrichment program (MEP), based upon Sager's contractual model of couple functioning, were evaluated using both self-inventories (DAS, MCI) and behavioral scales (MICS). Couples were randomly assigned to either no-treatment control conditions or the 20-hour long MEP. Evaluations of marital functioning of both groups were repeatedly done during the experimentation, at pretest, midtest, posttest and follow-up 1 (two months after completion of the program).

Surgical treatment of tennis elbow.

Nine patients with eleven cases of tennis elbow, recalcitrant to conservative therapy, underwent surgical reconstruction as described by Gardner in 1970. The average duration of symptoms preoperatively was 49 weeks. There were seven females and two males.

Prosthetic replacement in femoral neck fractures.

Despite refinement of the guidelines for the use of an endoprosthesis in the management of femoral neck fractures and for the use of bone cement, no universally accepted treatment regimen currently exists. In order to collect statistically significant data about the "unsolved fracture," prospective studies from several centers must be undertaken. However, until such data are available, we submit the following conclusions about the patient with a cemented endoprosthesis for a femoral neck fracture.