High frequency, direct shoot regeneration from greenhouse-derived leaf disks of six strawberry cultivars.

Leaf disks derived from either two-month-old GreenHouse-grown (GHD) strawberry plants or in vitro plantlets were cultured on MS media amended with 2 mg L(-1) Thidiazuron (TDZ), incubated for four weeks in the dark then for another four weeks under 16/8 h light regime. Regeneration capacity of leaf disks was compared to meristem-derived propagules in six strawberry cultivars. Direct shoot regeneration occurred in all tested cultivars with different frequencies depending on explant source.