Publications by authors named "Gillian P McMahon"
Background: The presence of calcium phosphate crystals such as basic calcium phosphate and calcium pyrophosphate dihydrate in intra-articular fluid is linked to a number of destructive arthropathies and detection of these deposits is often pivotal for early diagnosis and appropriate management of such disease.
Results: We describe the use of a calcium-sensitive dye, Fluo-4, to selectively label calcium-containing mineral deposits in synovial fluid, which can then be easily visualized using a standard fluorescence microscope. Furthermore, we have combined the fluorescent properties of the tagged crystals with flow cytometry as a fast and semi-quantitative method of detection.
An online SPE-LC method that can determine both anthracyclines and taxanes simultaneously in human serum samples is reported. The entire method of extraction, separation and UV detection was achieved online by column switching between an SPE column (Biotrap 500 (20 x 4 mm)) and an analytical column (Zorbax XDB C18, 150 x 4.6 mm, 5 microm) with a 23 min total cycle time.
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- The overuse of antibiotics in human medicine and large-scale veterinary drug administration in agriculture contribute to antibiotic resistance and human exposure through animal foods.
- The paper emphasizes the importance of detecting aminoglycosides and macrolides in food due to their common use in treating animal diseases and as growth promoters.
- It reviews analytical methods for extracting and testing these antibiotics in food, including techniques like solid-phase extraction, thin layer chromatography, and advanced liquid chromatography combined with mass spectrometry for precise analysis.
Article Synopsis
- The surface of superparamagnetic beads was enhanced with bisphosphonates.
- These modified beads are designed to selectively capture calcium phosphate crystals.
- This method is applied in complex biological fluids, such as synovial fluid.