Identification and purification of collagen-synthesizing polysomes with anti-collagen antibodies.

Antibodies against embryonic chick bone collagen were prepared in rabbits and were purified by affinity and ion exchange chromatography until collagen-specific and RNase-free. 125I-anti-collagen antibodies were used to locate the collagen-synthesizing polysomes of 8-day chick embryo wings and legs on sucrose gradients by measuring the polysome associated radioactivity. The 125I-anti-collagen antibodies bound predominantly to polysomes in the heavy region of sucrose gradients.

Role of airborne lead in increased body burden of lead in Hartford children.

The ingestion of airborne lead fallout is the mechanism responsible for increased lead body burdens found in 10 urban Connecticut children. The mean indoor lead levels found in housedust was 11,000 mug/g; highest concentrations occurred on windowsills and in floor dust. The mean lead content of Hartford street dirt was 1,200 mug/g; levels were highest near the street and next to the buildings.

Anion-exchange separation of metal ions in dimethyl sulphoxide-methanol-hydrochloric acid.

The effect of dimethyl sulphoxide on the anion-exchange behaviour of many metals m a methanolic system with hydrochloric acid was explored. Distribution coefficients for 26 elements were determined in a mixed solvent system of dimethyl sulphoxide-methanol-0.6M hydrochloric acid, in which the proportions of dimethyl sulphoxide and methanoi were varied.