Objective: To improve the fluorescence in situ hybridization (FISH) chromosomal analysis of human oocytes and first polar bodies.
Design: In situ chromosomal identification on isolated cells, with combinations of centromeric (or locus-specific) probes and whole-chromosome painting probes for chromosomes 9, 13, 16, 18, 21, and X.
Setting: Montpellier University Hospital.
Objective: To present and evaluate the use of a new ultra-fast multicolor primed in situ (PRINS) procedure for karyotyping human oocytes and first polar bodies.
Design: In situ chromosomal identification on isolated cells, using combinations of specific primers for chromosomes 1, 7, 9, 16, and 18 and fluorescent nucleotides.
Setting: Sixteen unfertilized oocytes were obtained from women participating in an IVF program.