Is multiplexed off-axis holography for quantitative phase imaging more spatial bandwidth-efficient than on-axis holography? [Invited].

Digital holographic microcopy is a thriving imaging modality that attracts considerable research interest due to its ability not only to create excellent label-free contrast but also to supply valuable physical information regarding the density and dimensions of the sample with nanometer-scale axial sensitivity. Three basic holographic recording geometries currently exist, including on-axis, off-axis, and slightly off-axis holography, each of which enables a variety of architectures in terms of bandwidth use and compression capacity. Specifically, off-axis holography and slightly off-axis holography allow spatial hologram multiplexing, enabling one to compress more information into the same digital hologram.

Optimal spatial bandwidth capacity in multiplexed off-axis holography for rapid quantitative phase reconstruction and visualization: erratum.

We correct a typo that repeated itself in several equations. Our previous results and conclusions are unchanged.

Integral refractive index imaging of flowing cell nuclei using quantitative phase microscopy combined with fluorescence microscopy.

We suggest a new multimodal imaging technique for quantitatively measuring the integral (thickness-average) refractive index of the nuclei of live biological cells in suspension. For this aim, we combined quantitative phase microscopy with simultaneous 2-D fluorescence microscopy. We used 2-D fluorescence microscopy to localize the nucleus inside the quantitative phase map of the cell, as well as for measuring the nucleus radii.

Four dimensional phase unwrapping of dynamic objects in digital holography.

We present a new four-dimensional phase unwrapping approach for time-lapse quantitative phase microscopy, which allows reconstruction of optically thick objects that are optically thin in a certain temporal point and angular view. We thus use all four dimensions of the dynamic quantitative phase profile acquired, including the angular dimension and the temporal dimension, in addition to the x-y dimensions. We first demonstrate the capabilities of this algorithm on simulative data, enabling the quantification of the reconstruction quality relative to both the ground truth and existing unwrapping approaches.

Six-pack off-axis holography.

We present a new holographic concept, named six-pack holography (6PH), in which we compress six off-axis holograms into a single multiplexed off-axis hologram without loss of magnification or resolution. The multiplexed hologram contains straight off-axis fringes with six different orientations, and can be generated optically or digitally. We show that since the six different complex wavefronts do not overlap in the spatial frequency domain, they can be fully reconstructed.

Rapid 3D Refractive-Index Imaging of Live Cells in Suspension without Labeling Using Dielectrophoretic Cell Rotation.

A major challenge in the field of optical imaging of live cells is achieving rapid, 3D, and noninvasive imaging of isolated cells without labeling. If successful, many clinical procedures involving analysis and sorting of cells drawn from body fluids, including blood, can be significantly improved. A new label-free tomographic interferometry approach is presented.

Angular phase unwrapping of optically thick objects with a thin dimension.

We present a new phase unwrapping approach, which allows reconstruction of optically thick objects that are optically thin from at least one viewing angle, by considering the information stored in the object phase maps captured from consecutive angles. Our algorithm combines 1-D phase unwrapping in the angular dimension with conventional 2-D phase unwrapping, to achieve unwrapping of the object from the optically thick perspective. We thus obtain quantitative phase imaging of objects that were previously impossible to image in certain viewing angles.

Video-rate processing in tomographic phase microscopy of biological cells using CUDA.

We suggest a new implementation for rapid reconstruction of three-dimensional (3-D) refractive index (RI) maps of biological cells acquired by tomographic phase microscopy (TPM). The TPM computational reconstruction process is extremely time consuming, making the analysis of large data sets unreasonably slow and the real-time 3-D visualization of the results impossible. Our implementation uses new phase extraction, phase unwrapping and Fourier slice algorithms, suitable for efficient CPU or GPU implementations.

Interferometric phase microscopy for label-free morphological evaluation of sperm cells.

Objective: To compare label-free interferometric phase microscopy (IPM) to label-free and label-based bright-field microscopy (BFM) in evaluating sperm cell morphology. This comparison helps in evaluating the potential of IPM for clinical sperm analysis without staining.

Design: Comparison of imaging modalities.