Multidrug resistance and high genotypic diversity in Campylobacter upsaliensis from household dogs in Metro Manila, Philippines.

Campylobacter upsaliensis is an emerging pathogen implicated in human gastroenteritis. Contact with pets, especially dogs, has been identified as a risk factor. Fourteen (14) C.

and Activity of L14 Strain Against DC3 Strain.

Introduction: Domestic poultry is a natural reservoir of , the host-pathogen interaction being predominantly asymptomatic. This study investigated whether chickens remain asymptomatic partly because of lactic acid bacteria (LAB).

Material And Methods: spp.

Isolation of Actinomycetes with Cellulolytic and Antimicrobial Activities from Soils Collected from an Urban Green Space in the Philippines.

Actinomycetes are one of the most important groups of soil bacteria that are recognized as sources of commercially important enzymes and antimicrobials. Actinomycetes, however, are largely underestimated and uncharacterized in underexplored habitats such as green spaces in urban areas. This study aimed to isolate actinomycetes from soils in the University of the Philippines Diliman campus and determine their cellulolytic and antimicrobial activities.

High Rates of Contamination of Poultry Meat Products with Drug-Resistant Campylobacter in Metro Manila, Philippines.

A total of 265 chicken parts were collected from 15 wet markets and 15 supermarkets in Metro Manila, Philippines. Campylobacter spp. was isolated on modified charcoal cefoperazone deoxycholate agar plates and identified through biochemical tests and PCR amplification of genus- and species-specific genes.

Phenotypic and transcriptional profiling in Entamoeba histolytica reveal costs to fitness and adaptive responses associated with metronidazole resistance.

Antimicrobial chemotherapy is critical in the fight against infectious diseases caused by Entamoeba histolytica. Among the drugs available for the treatment of amebiasis, metronidazole (MTZ) is considered the drug of choice. Recently, in vitro studies have described MTZ resistance and the potential mechanisms involved.

Transcriptional and functional analysis of trifluoromethionine resistance in Entamoeba histolytica.

Objectives: Drug resistance in parasitic protozoa is an obstacle to successful chemotherapy. Understanding how pathogens respond to drugs is crucial in preventing resistance. Previously, we have shown that in Entamoeba histolytica, methionine γ-lyase (EhMGL) downregulation results in trifluoromethionine resistance.

Mechanism of trifluoromethionine resistance in Entamoeba histolytica.

Objectives: To determine the mechanism of trifluoromethionine resistance in Entamoeba histolytica and evaluate the impact of acquired drug resistance on virulence.

Methods: Trifluoromethionine-resistant amoebae were selected in vitro and examined for cross-resistance to antiamoebic drugs, stability of resistance, methionine γ-lyase (MGL) activity, cell adhesion and virulence. Targeted gene silencing was performed to confirm the role of EhMGL.