Purpose: Ki-67 is recommended by international/national guidelines for risk stratification in early breast cancer (EBC), particularly for defining "intermediate risk," despite inter-laboratory/inter-observer variability and cutoff uncertainty. We investigated Ki-67 (> 10%- < 40%, determined locally) as a prognostic marker for intermediate/high risk in EBC, pN0-1 patients.
Methods: This prospective, non-interventional, real-world study included females ≥ 18 years, with pN0/pN1mi/pN1, HR+ , HER2-negative EBC, and locally determined Ki-67 ranging 10%-40%.
Objective: What are the patients attitudes about their fertility and about the counselling process at the time when fertility preservation counselling is performed?
Study Design: A survey regarding fertility concerns and counselling performance in relation to the chosen fertility preservation procedure such as no treatment, GnRH agonists, and freezing of ovarian tissue or oocytes/zygotes was prospectively conducted in four university centres and one private centre, all belonging to the network FertiPROTEKT in Germany and Switzerland.
Results: All women (n=145) received a questionnaire at the first counselling appointment. The mean age of the patients was 30 years (±5.
This investigation was conducted to quantify the purine excretion of cows and to evaluate milk allantoin as an estimator of synthesis of microbial protein in the rumen. Concentrations in plasma of allantoin and uric acid and their excretion in urine and milk were determined using 16 Holstein cows at 21 to 315 d of lactation and yielding 8 to 36 kg/d of milk. The mean daily intake of a 55:45 roughage and concentrate diet was 16.
View Article and Find Full Text PDFZentralbl Veterinarmed A
May 1994
The purpose of this paper was to study the effect of rumen metabolism on the concentration of certain cyclic N-compounds in blood plasma. The plasma from two groups of lambs in the phase of rumen development during early and late weaning was used. As a second model, samples from steers fed at maintenance and after emptying of the rumen via cannula were used.
View Article and Find Full Text PDFZentralbl Veterinarmed A
August 1993
Experiments in piglets indicate that the catabolic metabolism of purines is only developed during the first 4 weeks of life. During this time the catalytic activity of uricase in liver homogenates is increased about 5.5 fold (p < 0.
View Article and Find Full Text PDF1. The activity of xanthine oxidase in liver samples of baby pigs up to 4 weeks of age was investigated. On the 3rd day of life the turnover of hypoxanthine and of uric acid were measured after intravenous injection of 3H- and 14C-labelled tracers into animals kept at normal (32 degrees C) and reduced (20 degrees C) ambient temperature.
View Article and Find Full Text PDFZentralbl Veterinarmed A
September 1992
With two groups of dairy cows (A: 12 German Black and White- and 6 German Simmental Cows; B: 16 German Black and White- and 11 German Simmental Cows) allantoin concentrations of blood plasma (A) and milk (A and B) were analysed by HPLC. There exists a close correlation (r = 0.758) between allantoin concentration in blood plasma (370.
View Article and Find Full Text PDFIn milk and blood plasma samples of 6 German Simmental and 12 German Black and White heifers it was investigated, whether purine and pyrimidine compounds are suitable indicators of the microbial protein synthesis in the rumen. Therefore the secreted quantities in milk and the concentration in blood plasma are correlated with energy intake. The results indicated significant correlation coefficients for both the secretion quantity of allantoin in milk (r = 0.
View Article and Find Full Text PDFIn eight Dalmatian dogs exogenous effects (dietary purine, xylit infusion) on plasma uric acid were examined and relationships between purine intake and excretion were established. Increasing purine intake resulted in a linear increase in renal excretion of urate (r = 0.952) and a less steep increase of allantoin (r = 0.
View Article and Find Full Text PDFIn a nutritional tolerance study 36 young dogs were fed over 52 weeks high or low purine diets at an average paired feeding intake of 0.6 and 80 mg purine-N/MJ per day. The high purine diet resulted in a significant decrease of growth rate by -23% (Beagles) and -38% (Dalmatians) and of feed efficiency (-29 and -42%).
View Article and Find Full Text PDFThe number of hepatic peroxisomes, cores, marginal plates, and microperoxisomes in piglets increases from a low level at birth, compared with other species e.g. the rat, to day 28 of life.
View Article and Find Full Text PDFZentralbl Veterinarmed A
February 1990
In order to study mechanisms in ruminants responsible for glucocorticoid-induced hyperglycemia the effects of a single intravenous dose of a glucocorticoid on energy metabolism were investigated by measuring plasma levels of glucose, lactate, free fatty acids (FFA), 3-hydroxybutyrate (3-HOB) and acetoacetate (AcAc). Experiments were performed on four castrated male African pygmy goats at two metabolic states: "fed" with a single daily meal of hey and "fasted" with starvation for 3 days. The untreated goats served as their own control.
View Article and Find Full Text PDFComp Biochem Physiol B
June 1989
1. In eight Dalmatian dogs low and high purine intakes resulted in plasma urate levels from 25 to 185 mumol/l. 2.
View Article and Find Full Text PDFComp Biochem Physiol B
February 1988
1. The activity of uricase, the densities of peroxisomes and cores in liver samples of baby pigs up to 4 weeks of age were investigated. 2.
View Article and Find Full Text PDFSerum magnesium concentrations were followed during the course of coronary infarction and were correlated to the event on the basis of the creatinine kinase activity E.C. no.
View Article and Find Full Text PDFJ S Afr Vet Assoc
March 1986
Comp Biochem Physiol B
December 1986
In order to determine the main organ of fatty acid synthesis de novo in the dog, glucose-C incorporation into fatty acids and glucose-1-C oxidation to CO2 were measured by means of glucose-U-14C and glucose-1-14C in samples of adipose and hepatic tissues of 16 animals. For comparison of the two organs the data were referred to the respective DNA content. The rate of glucose-C incorporation into fatty acids of adipose tissue exceeded that of hepatic tissue about 1000-fold.
View Article and Find Full Text PDFComp Biochem Physiol A Comp Physiol
February 1987
Uric acid uptake by erythrocytes of Beagle and Dalmatian dogs has been measured, using (2-14C) uric acid. Uptake was characterized by a fast and a slow component. Urate uptake was inhibited by certain purine and pyrimidine derivatives and by anion transport inhibitors.
View Article and Find Full Text PDFThe metabolization of D-lactate was examined in volunteers by means of renal excretion after intake of DL-lactate, and in rats as well as in rat liver in vitro by the oxidation rate of 14C-D-lactate to 14CO2. In five volunteers after intake of DL-lactate containing 1.60 to 6.
View Article and Find Full Text PDFAfter introducing into developmental aspects of "Dalmatian dog research" uric acid excretion is explained on the basis of new results by the loss of a specific transport system in liver cell membranes. Therefore, uricase which is present in normal activities is supplied only to a limited extent with uric acid for the transformation into the easily soluble allantoin. As indicated by feeding experiments, the plasma urate level is increased from about 32 mumol/l (no purine intake) up to 150-200 mumol/l at very high levels of purine intake.
View Article and Find Full Text PDFComp Biochem Physiol B
November 1985
In experiments with rumen epithelium incubated in vitro in the presence of butyrate, the ketogenic effect of glucose was shared by epimeric monosaccharides but not by non-metabolizable analogues. 14C from glucose was not incorporated into ketone bodies. Malate increased ketogenesis from butyrate and decreased its oxidation, pyruvate and NH4+ had the opposite effect, and malonate inhibited both processes.
View Article and Find Full Text PDFComp Biochem Physiol B
November 1985
In rats given D[u-14C]-labelled DL-lactate with 3.8-13.4 mmol D-lactate per kg 0.
View Article and Find Full Text PDFThe uptake rate of uric acid into liver slices of the Dalmatian dog was found to be significantly lower compared with that in the beagle. Since there was no difference in the hepatic uricase activity between the two breeds, the abnormality of uric acid metabolism in the Dalmatian dog is considered to result from a defective hepatic transport system for uric acid.
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