The organization of the araBAD operon of Escherichia coli.

The nucleotide (nt) sequence of the araBAD operon of Escherichia coli B/r has been determined. The nt sequence predicts a transcript of about 4250 nt. The coding regions of araB, araA and araD genes were identified by partial amino acid (aa) sequences of the purified proteins and their cleavage products.

Mechanism of araC autoregulation and the domains of two overlapping promoters, Pc and PBAD, in the L-arabinose regulatory region of Escherichia coli.

The DNA-protein contact sites in the ara regulatory region, which contains the promoters for araBAD and araC, have been determined for araC protein, the cyclic AMP-binding protein, and RNA polymerase, by using the methylation protection and DNase I protection methods. The functional significance of binding was assessed by correlating the state of occupancy of these sites with promoter activity in transcription initiation. Our results suggest that the basis for araC autoregulation is that araC protein, in either its activator (P2) or repressor (P1) form, acts as a repressor for araC, by binding to the RNA polymerase attachment site at the araC promoter.

L-Ribulokinase from an initiator constitutive mutant.

The N-terminal sequence of l-ribulokinase (EC 2.7.1.

In vitro activation of the transcription of araBAD operon by araC activator.

The transcription of araBAD operon requires araC activator and cyclic AMP. D-Fucose inhibits ara mRNA synthesis. Our results indicate that the positive control by araC activator is exerted at the level of transcription.

Properties of D-arabinose isomerase purified from two strains of Escherichia coli.

d-Arabinose isomerase (EC 5.3.1.