Semiautomated Production of Cell-Free Biosensors.

Cell-free synthetic biology biosensors have potential as effective diagnostic technologies for the detection of chemical compounds, such as toxins and human health biomarkers. They have several advantages over conventional laboratory-based diagnostic approaches, including the ability to be assembled, freeze-dried, distributed, and then used at the point of need. This makes them an attractive platform for cheap and rapid chemical detection across the globe.

The Progression of Developmental Language Disorder Terminology: A Scoping Review of American Speech-Language-Hearing Association Journals.

Purpose: A 2017 CATALISE project resulted in consensus on using the term "developmental language disorder" (DLD) to describe children with unexplained language impairment. Since then, it is unclear how researchers have identified DLD and implemented DLD terminology. The current study is a scoping review to better understand the implementation of DLD terminology.

Effect of SMS Ward Round Notifications on Inpatient Experience in Acute Medical Settings: Retrospective Cohort Study.

Background: Ward rounds are an essential component of inpatient care. Patient participation in rounds is increasingly encouraged, despite the occasional complicated circumstances, especially in acute care settings.

Objective: This study aimed to evaluate the effect of real-time ward round notifications using SMS text messaging on the satisfaction of inpatients in an acute medical ward.

Exploring the Views of Young People, Including Those With a History of Self-Harm, on the Use of Their Routinely Generated Data for Mental Health Research: Web-Based Cross-Sectional Survey Study.

Background: Secondary use of routinely collected health care data has great potential benefits in epidemiological studies primarily due to the large scale of preexisting data.

Objective: This study aimed to engage respondents with and without a history of self-harm, gain insight into their views on the use of their data for research, and determine whether there were any differences in opinions between the 2 groups.

Methods: We examined young people's views on the use of their routinely collected data for mental health research through a web-based survey, evaluating any differences between those with and without a history of self-harm.

Efficacy of Combined CD38 and PD1 Inhibition with Isatuximab and Cemiplimab for Relapsed/Refractory NK/T-Cell Lymphoma.

This study aimed to assess the efficacy and safety of combining cemiplimab, an anti-PD1 antibody, with isatuximab, an anti-CD38 antibody, in relapsed or refractory extranodal NK/T-cell lymphoma (R/R ENKTL). The hypothesis was that CD38 blockade could enhance the antitumor activity of PD1 inhibitors. Eligible patients received cemiplimab (250 mg on days 1 and 15) and isatuximab (10 mg/kg on days 2 and 16) intravenously every four weeks for six cycles.

All segments are created equal: locoregional recurrence and survival after single versus multi-segment resection in patients with clinical stage IA ≤ 2 cm non-small cell lung cancer.

Objectives: Compare oncologic outcomes between single-segment and multi-segment resections in patients with clinical stage IA1 and IA2 non-small cell lung cancer.

Methods: A retrospective review (2011-2022) was conducted using a prospectively maintained database. Patients undergoing anatomical segmentectomy for clinical stage IA ≤ 2 cm non-small cell lung cancers were included.

Dielectric-Free Molybdenum Disulfide Transistors with In-Plane Gates.

In this work, we realize bilayer molybdenum disulfide (MoS) transistors with in-plane gates on sapphire substrates. Through sequential transferring of MoS, e-beam lithography, and metal lift-off, a device with channel width/length of 500:400 nm is fabricated. With a 250 nm separation between the in-plane gate and MoS channel, a drain current as high as 37 μA with a clear saturation region is observed.

A human histidyl-tRNA synthetase splice variant therapeutic targets NRP2 to resolve lung inflammation and fibrosis.

Interstitial lung disease (ILD) consists of a group of immune-mediated disorders that can cause inflammation and progressive fibrosis of the lungs, representing an area of unmet medical need given the lack of disease-modifying therapies and toxicities associated with current treatment options. Tissue-specific splice variants (SVs) of human aminoacyl-tRNA synthetases (aaRSs) are catalytic nulls thought to confer regulatory functions. One example from human histidyl-tRNA synthetase (HARS), termed HARS because the splicing event resulted in a protein encompassing the WHEP-TRS domain of HARS (a structurally conserved domain found in multiple aaRSs), is enriched in human lung and up-regulated by inflammatory cytokines in lung and immune cells.

Oxygen-excluded nanoimaging of polymer blend films.

Polymer blend films exhibit unique properties and have applications in various fields. However, understanding their nanoscale structures and polymer component distributions remains a challenge. To address this limitation, we have developed a super-resolution fluorescence microscopy-based technique called oxygen-excluded nanoimaging.

Shaping early neural development by timed elevated tissue oxygen tension: Insights from multiomic analysis on human cerebral organoids.

Oxygen plays a critical role in early neural development in brains, particularly before establishment of complete vasculature; however, it has seldom been investigated due to technical limitations. This study uses an in vitro human cerebral organoid model with multiomic analysis, integrating advanced microscopies and single-cell RNA sequencing, to monitor tissue oxygen tension during neural development. Results reveal a key period between weeks 4 and 6 with elevated intra-organoid oxygen tension, altered energy homeostasis, and rapid neurogenesis within the organoids.

Targeting the NPY/NPY1R signaling axis in mutant p53-dependent pancreatic cancer impairs metastasis.

Pancreatic cancer (PC) is a highly metastatic malignancy. More than 80% of patients with PC present with advanced-stage disease, preventing potentially curative surgery. The neuropeptide Y (NPY) system, best known for its role in controlling energy homeostasis, has also been shown to promote tumorigenesis in a range of cancer types, but its role in PC has yet to be explored.

Inhibitory immunoreceptors CD300a and CD300lf cooperate to regulate mast cell activation.

Mast cells (MCs) play a central role in allergic immune responses. MC activation is regulated by several inhibitory immunoreceptors. The CD300 family members CD300a and CD300lf recognize phospholipid ligands and inhibit the FcεRI-mediated activating signal in MCs.

Modulation of germinal center and antibody dynamics via ipsilateral versus contralateral immunization against SARS-CoV-2.

Human clinical trials have reported immunological outcomes can differ between ipsilateral (same side) and contralateral (alternate sides) prime-boost vaccination. However, our mechanistic understanding of how keeping or shifting the anatomical sites of immunization impacts the resultant germinal centers (GCs) and antibody responses is limited. Here, we use an adjuvanted SARS-CoV-2 spike vaccine to dissect GC dynamics in draining lymph nodes and serological outcomes following ipsilateral or contralateral prime-boost vaccination in C57BL/6 mice.

Association between PM2.5 and skin redness features in Taiwan.

Air pollution, particularly fine particulate matter (PM2.5), has been associated with various health issues, but its effects on skin health, specifically skin redness, remain underexplored. This study aims to examine the relationship between PM2.

Development of the Follow-Up Discourse Observation Protocol (FUDOP) for Characterizing Instructor Active Learning Follow-Up Behaviors.

Instructors often provide feedback to their class in multiple ways. One way is through their follow-up behaviors, which are the specific strategies instructors implement after active learning activities. These behaviors could play an important role in student learning as students receive feedback from the instructor.

gen. nov., sp. nov., a novel halophilic bacterium isolated from tidal flat sediment.

A novel bacterial strain, MJW-29, was isolated from tidal flat sediment in Gochang, Republic of Korea. The isolate is Gram-stain-negative, facultatively anaerobic, gliding motile and short rods. The strain MJW-29 is oxidase and catalase positive.

A telomere-to-telomere phased genome of an octoploid strawberry reveals a receptor kinase conferring anthracnose resistance.

Background: Cultivated strawberry (Fragaria xananassa Duch.), an allo-octoploid species arising from at least 3 diploid progenitors, poses a challenge for genomic analysis due to its high levels of heterozygosity and the complex nature of its polyploid genome.

Results: This study developed the complete haplotype-phased genome sequence from a short-day strawberry, 'Florida Brilliance' without parental data, assembling 56 chromosomes from telomere to telomere.

Comparison of health-related quality of life trajectories in older breast cancer survivors and noncancerous controls over 10 years: A SEER-MHOS analysis.

Purpose: This study analyzed the long-term effects of cancer on the health-related quality of life (HRQOL) of older breast cancer survivors for 10 years, including a control group to distinguish between the impacts of cancer treatment and aging.

Methods: The SEER-MHOS data resource was used in the analysis. Cases were 674 older women diagnosed with breast cancer in 1998-2015 at age 65 and older and participated in Medicare Health Outcomes Survey within 24 months before their cancer diagnosis and at least once within 10 years of post-diagnosis.

Construction of Chitosan Oligosaccharide-Coated Nanostructured Lipid Carriers for the Sustained Release of Strontium Ranelate.

Background: Strontium ranelate (SR) is an effective bone regeneration drug; however, its low bioavailability and strong hydrophilicity cause a strong cytotoxicity, venous thrombosis, and allergic reactions when administered in its free form. This study aims to enhance the SR bioavailability by utilizing nanostructured lipid carriers (NLC) as a drug delivery system (DDS).

Methods: To improve the drug delivery efficiency and sustained release of the NLC, their surfaces were coated with chitosan oligosaccharide (COS), a natural polymer.

Bio-Inspired Ionic Sensors: Transforming Natural Mechanisms into Sensory Technologies.

Many natural organisms have evolved unique sensory systems over millions of years that have allowed them to detect various changes in their surrounding environments. Sensory systems feature numerous receptors-such as photoreceptors, mechanoreceptors, and chemoreceptors-that detect various types of external stimuli, including light, pressure, vibration, sound, and chemical substances. These stimuli are converted into electrochemical signals, which are transmitted to the brain to produce the sensations of sight, touch, hearing, taste, and smell.

Guanfacine Use in the ICU for Management of Sedation Weaning.

Recent evidence highlights the increasing utilization of guanfacine in the intensive care unit. While dexmedetomidine is a widely used sedative and anti-anxiety agent in the intensive care unit, prolonged use can lead to withdrawal effects when attempting to reduce the dosage. This has generated interest in using guanfacine to manage agitation in patients being weaned off dexmedetomidine.

Near-Peer Mentorship in a Medical-Student Led Oncology and Regenerative Medicine Research Education Program Increases STEM Interest in Underrepresented High School Students.

Students from underrepresented backgrounds are less likely to pursue careers in healthcare and research. Despite the existence of many pipeline programs to reduce this disparity, there is still a need to effectively increase the diversity of the STEM workforce. Our research education program for underrepresented high school students attempts to bridge this gap by implementing the near-peer mentorship strategy by incorporating medical students in roles as program leaders and mentors.

Carbapenem-Resistant, Virulence Plasmid-Harboring Klebsiella pneumoniae, United States.

Carbapenem-resistant and virulence plasmid-harboring Klebsiella pneumoniae (pVir-CRKP) has emerged and spread globally, yet clinical investigations from the United States remain limited. We conducted a genomic analysis of 884 unique carbapenem-resistant K. pneumoniae isolates from a multicenter US cohort and identified 6 pVir-CRKP isolates, including 2 sequence type (ST) 23, 2 ST893, and 2 ST11 isolates.

Diagnostic accuracy of IgG 4/IgG and IgG 4 in patients with IgG 4-related disease: a meta-analysis.

Objective: This study aimed to evaluate and compare the diagnostic performance of immunoglobulin G4/Immunoglobulin G (IgG 4/IgG) and immunoglobulin G4 (IgG 4) alone in identifying immunoglobulin G4-related disease (IgG 4-RD).

Methods: A systematic review and meta-analysis were conducted using data from Medline, Embase, and the Cochrane Library from inception to November 2024. Two meta-analyses were performed to assess the diagnostic accuracies of IgG 4/IgG and IgG 4 in IgG 4-RD patients.

recording and stimulation performance of multi-electrode arrays passivated with plasma-enhanced atomic layer-deposited metal oxides.

To achieve an intimate contact between neuronal cells and the electrode in non-invasive platforms intended for neurological research, in this study, we fabricated a raised-type Au multi-electrode array (MEA) by employing nanoscale-thick indium-tin oxide (ITO; 50 nm) as a track layer and plasma-enhanced atomic layer-deposited (PEALD) AlO (30-60 nm) and HfO (20 nm) as passivation layers. The PEALD AlO-passivated Au MEA was subsequently modified with electrodeposited AuPt nanoparticles (NPs) and IrO to demonstrate the passivation capability and chemical resistance of AlO to Au-, Pt-, and IrO NP-containing electrolytes. AlO-passivated and IrO/AuPt-modified MEAs could resolve optogenetically activated spikes and spontaneous activities with a root-mean-square noise level of 2.