Cortical oscillations and event-related brain potentials during the preparation and execution of deceptive behavior.

Deception often occurs in response to a preceding cue (e.g., a precarious question) alerting us about the need to subsequently lie.

You're Beautiful When You Smile: Event-Related Brain Potential (ERP) Evidence of Early Opposite-Gender Bias in Happy Faces.

Studies of social cognition have shown gender differences regarding human face processing. One interesting finding is the enhanced processing of opposite-gender faces at different time stages, as revealed by event-related brain potentials. Crucially, from an evolutionary perspective, such a bias might interact with the emotional expression of the face.

The FOXP3 Pro-Inflammatory T Cell: A Potential Therapeutic Target in Crohn's Disease.

Background & Aims: The incidence of Crohn's disease (CD) continues to increase worldwide. The contribution of CD4 cell populations remains to be elucidated. We aimed to provide an in-depth transcriptional assessment of CD4 T cells driving chronic inflammation in CD.

Tasting inhibition: A proof-of-concept study of the food stop-signal game.

Self-Control is an important skill in everyday life when attention is automatically drawn toward certain stimuli. For instance, food stimuli automatically capture visual attention and are processed preferentially. Therefore, efficient response inhibition is crucial to refrain from careless overeating.

Of ugly gains and happy losses: An event-related potential study of interactions of the intrinsic and acquired valence of emotional pictures.

During the last decades, event-related potential research on the processing of intrinsic and acquired valence has made great progress, but the two dimensions rarely varied simultaneously. Only that way, however, can we investigate whether the acquisition of extrinsic valence varies with intrinsic valence and whether intrinsic and acquired valence share the same brain mechanisms. Forty-five participants performed associative learning of gains and losses, using pictures varying on intrinsic valence (positive, negative) and outcome (90 % gain, 50 %/50 %, 90 % loss).

Hypomethylation and Overexpression of Th17-Associated Genes is a Hallmark of Intestinal CD4+ Lymphocytes in Crohn's Disease.

Background: The development of Crohn's disease [CD] involves immune cell signalling pathways regulated by epigenetic modifications. Aberrant DNA methylation has been identified in peripheral blood and bulk intestinal tissue from CD patients. However, the DNA methylome of disease-associated intestinal CD4+ lymphocytes has not been evaluated.

Immune checkpoint inhibitor-induced diabetes mellitus with nivolumab.

Immune checkpoint inhibitors (ICIs) have been increasingly used in the treatment of various advanced cancers; however, therapy can be complicated by immune-related adverse events (irAEs). We present the case of a man in his 40s, with metastatic melanoma treated with nivolumab immunotherapy who developed ICI-induced diabetes mellitus (ICI-DM). Hyperglycaemia in the absence of ketoacidosis was incidentally noted when he presented to the emergency department for review of an urticarial rash.

Attentional tuning of valent word forms.

Using event-related brain potentials (ERPs), we tested the hypothesis of valent word forms (Kissler et al., 2006, Prog Brain Res) stating that the mere visual shapes of emotional words acquire valence through numerous co-occurrences with affective activation over an experienced reader's life. Thereby, associations between neuronal representations of visual word forms and affect are strengthened.

G9a Modulates Lipid Metabolism in CD4 T Cells to Regulate Intestinal Inflammation.

Background & Aims: Although T-cell intrinsic expression of G9a has been associated with murine intestinal inflammation, mechanistic insight into the role of this methyltransferase in human T-cell differentiation is ill defined, and manipulation of G9a function for therapeutic use against inflammatory disorders is unexplored.

Methods: Human naive T cells were isolated from peripheral blood and differentiated in vitro in the presence of a G9a inhibitor (UNC0642) before being characterized via the transcriptome (RNA sequencing), chromatin accessibility (assay for transposase-accessible chromatin by sequencing), protein expression (cytometry by time of flight, flow cytometry), metabolism (mitochondrial stress test, ultrahigh performance liquid chromatography-tandem mas spectroscopy) and function (T-cell suppression assay). The in vivo role of G9a was assessed using 3 murine models.

Event-related potentials of food-induced blindness in the rapid serial visual presentation paradigm.

Emotion-induced blindness is known as the impairment in the identification of targets that follow shortly after emotional distractors in the rapid serial visual presentation (RSVP) paradigm. Besides negative or erotic stimuli, food distractors have also been found to elicit a similar effect. This indicates an attentional bias for food stimuli in the temporal dimension of visual attention, which is highly relevant in the context of eating behaviour.

Event-related brain potentials of temporal generalization: The P300 span marks the transition between time perception and time estimation.

There has been a long-standing debate on where on the time axis the transition between time perception and time estimation (i.e., the cognitive reconstruction of time) can be located.

Attention to affect 2.0: Multiple effects of emotion and attention on event-related potentials of visual word processing in a valence-detection task.

Here we continue recent work on the specific mental processes engaged in a valence-detection task. Fifty-seven participants responded to one predefined target level of valence (negative, neutral, or positive), and ignored the remaining two levels. This enables more precise fine-tuning of neuronal pathways, compared to valence categorization where attention is divided between different levels of valence.

Putative Phenotypically Neutral Genomic Insertion Points in Prokaryotes.

The barriers to effective genome editing in diverse prokaryotic organisms have been falling at an accelerated rate. As editing becomes easier in more organisms, quickly identifying genomic locations to insert new genetic functions without disrupting organism fitness becomes increasingly useful. When the insertion is noncoding DNA for applications such as information storage or barcoding, a neutral insertion point can be especially important.

Immune checkpoint inhibitor-induced diabetes mellitus with pembrolizumab.

An 81-year-old woman with a background of metastatic melanoma on pembrolizumab with no history of diabetes was brought into the emergency department with polyuria, polydipsia and weight loss. The initial assessment was consistent with severe diabetic ketoacidosis (DKA) and prerenal acute kidney injury with no clinical evidence of infection. The patient was treated with fluid resuscitation and an insulin infusion and eventually transitioned to a basal-bolus insulin regime, which was continued after discharge.

The ChpR transcriptional regulator of senses 3,5,6-trichloropyridinol, a degradation product of the organophosphate pesticide chlorpyrifos.

The global use of organophosphate insecticides (OPPs) and the growing concern of off-target side effects due to OPP exposure has prompted the need for sensitive and economical detection methods. Here we set out to engineer a previously identified OPP responsive transcription factor, ChpR, from to respond to alternative OPPs and generate a repertoire of whole-cell biosensors for OPPs. The ChpR transcription factor and cognate promoter P have been shown to activate transcription in the presence of the OPP chlorpyrifos (CPF).

Long non-coding RNA ACTA2-AS1 promotes ductular reaction by interacting with the p300/ELK1 complex.

Background & Aims: Biliary disease is associated with a proliferative/fibrogenic ductular reaction (DR). p300 is an epigenetic regulator that acetylates lysine 27 on histone 3 (H3K27ac) and is activated during fibrosis. Long non-coding RNAs (lncRNAs) are aberrantly expressed in cholangiopathies, but little is known about how they recruit epigenetic complexes and regulate DR.

Effects of the calorie content of visual food stimuli and simulated situations on event-related frontal alpha asymmetry and event-related potentials in the context of food choices.

Approach and avoidance tendencies play an important role in everyday food choices when choosing between high-caloric, rather unhealthy, and low-caloric, rather healthy options. On a neuronal level, approach and avoidance motivation have been associated with asymmetrical activity of the frontal cortex, often quantified by alpha power averaged over several seconds of resting electroencephalogram (EEG). Going beyond the analysis of resting EEG, the present study aimed to investigate asymmetrical frontal activity in direct response to food stimuli in an event-related design and in combination with event-related potentials (ERPs).

BMI1 maintains the Treg epigenomic landscape to prevent inflammatory bowel disease.

FOXP3+ Tregs are expanded within the inflamed intestine of human Crohn's disease, yet FOXP3-mediated gene repression within these cells is lost. The polycomb repressive complexes play a role in FOXP3 target gene regulation, but deeper mechanistic insight is incomplete. We have now specifically identified the polycomb-repressive complex 1 (PRC1) family member, BMI1 in the regulation of a proinflammatory enhancer network in both human and murine Tregs.

Helpless against food cues: the influence of pro- and anti-sugar videos on instrumental food-seeking behaviour in a Pavlovian-to-instrumental transfer paradigm.

Objective: External food cues can trigger food seeking by means of associative Stimulus-Outcome-Response learning mechanisms. These mechanisms can contribute to cued overeating. The present study aims at investigating if (cued) food-seeking behaviour can be influenced by pro- and anti-sugar videos.

barCoder: a tool to generate unique, orthogonal genetic tags for qPCR detection.

Background: Tracking dispersal of microbial populations in the environment requires specific detection methods that discriminate between the target strain and all potential natural and artificial interferents, including previously utilized tester strains. Recent work has shown that genomic insertion of short identification tags, called "barcodes" here, allows detection of chromosomally tagged strains by real-time PCR. Manual design of these barcodes is feasible for small sets, but expansion of the technique to larger pools of distinct and well-functioning assays would be significantly aided by software-guided design.

Flucloxacillin and paracetamol induced pyroglutamic acidosis.

A 75-year-old woman was admitted to a regional hospital with an acute kidney injury (AKI) and nausea on a background of recent treatment for bacteraemia secondary to pneumonia. The treatment thereof resulted in a high anion gap metabolic acidosis (HAGMA). The pneumonia was initially treated with intravenous piperacillin and tazobactam and the patient transferred to a tertiary hospital.