First molecular report of Moniezia expansa in small ruminants of Pakistan with epidemiological insight.

The members of genus Moniezia are the common parasites of livestock in tropical areas. The tapeworm, Moniezia expansa is commonly found in the gastrointestinal tract of the small and large ruminants. The present study focused on reporting the prevalence of M.

Pseudorabies Virus Prevalence in Lung Samples of Hunted Wild Boars in Northwestern Greece.

Aujeszky's Disease, caused by the pseudorabies virus (PRV), is an acute, often fatal disease affecting mainly pigs and incidentally other animals. While eradicated in several countries, PRV persists in wild boar populations, posing a risk to domestic pigs. This study investigates PRV prevalence in wild boars in the region of Epirus, located in the northwest of Greece.

Comparison of Molecularly Identified Resistant and Susceptible Johnsongrass ( L.) Populations at Gene, in the Absence and Presence of Field Crops.

Background/objectives: Johnsongrass () is an erect tetraploid, perennial, C4 grass weed species categorized among the world's most noxious weeds due to its high competitive ability against crops and the increased number of field-evolved herbicide-resistant populations. The aim of the present study was to assess the growth rate and performance of resistant (R) johnsongrass genotypes hosting Trp574Leu target-site cross-resistance at gene, inhibiting various herbicides, compared to susceptible (S) conspecific weeds, in the absence and presence of corn or sunflower antagonism.

Methods: The aboveground biomass, tiller, and rhizome production ability of one S and one R johnsongrass population with a Trp574-Leu substitution conferring cross-resistance to ALS-inhibiting herbicides were compared under non-competitive conditions.

Efficacy Of Transfluthrin Varies By Species And Placement In A Warm Temperate Mediterranean Environment1.

Spatial repellents may protect personnel in delimited areas from incursion of vector or nuisance Diptera, such as mosquitoes and sand flies. We investigated the efficacy of resin diffusers impregnated with transfluthrin in protecting 2 simulated military field scenarios (partially opened tents and open bivouacs lacking shelter) from natural populations of Culex species mosquitoes and Phlebotomus species sand flies. Using a benchmark efficacy of 61% expected reduction from a recent literature survey, we found that transfluthrin protected bivouacs from sand flies (74.

Utility of dairy microbiome as a tool for authentication and traceability.

Milk microbiome contributes substantially to the formation of specific organoleptic and physicochemical characteristics of dairy products. The assessment of the composition and abundance of milk microbiota is a challenging task strongly influenced by many environmental factors. Specific dairy products may be designated by the Protected Designation of Origin (PDO) and Protected Geographical Indication (PGI) labeling, which however, occasionally fail to differentiate them according to specific quality characteristics, which are defined by different microbiota-driven reactions.

Heat Hardening Ameliorates Apoptotic and Inflammatory Effects Through Increased Autophagy in Mussels.

The severity, frequency, and duration of extreme events, in the context of global warming, have placed many marine ecosystems at high risk. Therefore, the application of methods that can mediate the impacts of global warming on marine organisms seems to be an emerging necessity in the near term. In this context, enhancing the thermal resilience of marine organisms may be crucial for their sustainability.

Prevalence of Neospora caninum and associated risk factors in semi-intensive female water buffaloes reared in Greece, using an ELISA kit on milk samples.

Neospora caninum is an apicomplexan (family: Sarcocystidae) protozoan parasite with a global distribution. In the N. caninum life cycle, dogs and other related canids are the definitive hosts, while other animals such as water buffaloes (Bubalus bubalis) constitute the intermediate host for this parasite.

First detection of Cupriavidus gilardii in a bovine neonatal diarrhea outbreak.

Background: Cupriavidus gilardii is an aerobic, gram-negative, motile, glucose-nonfermenting bacillus, first described in 1999. Typically, it exhibits low pathogenicity in humans, causing opportunistic infections primarily in individuals with compromised immune systems. This bacterium has been also found in various environmental sources such as plants and contaminated soils.

Seasonal energy investment and metabolic patterns in a farmed fish.

The present research focuses on the seasonal changes in the energy content and metabolic patterns of red porgy (Pagrus pagrus) sampled in a fish farm in North Evoikos Gulf (Greece). The study was designed in an effort to evaluate the influence of seasonality in several physiological feauteres of high commercial importance that may affect feed intake and growth. We determined glycogen, lipids and proteins levels, and cellular energy allocation (CEA) as a valuable marker of exposure to stress, which integrates available energy (Ea) and energy consumption (Ec).

Molecular characterization of Lernathropus kroyeri from intensive aquaculture and pathophysiology of infested sea bass.

The copepod Lernathropus kroyeri constitutes one of the major parasites for the Mediterranean aquaculture, infesting the sea bass Dicentrarchus labrax causing thus disruptions of growth performance and occasionally mortalities. Despite the large spread and the high frequency of this parasite in mariculture farms of Eastern Mediterranean, L. kroyeri genetic profile from aquaculture as well as the pathophysiological response of D.

Histopathology and Phylogeny of the Dinoflagellate and the Epibiotic Peritrich Ciliate sp. Infecting the Blue Crab in the Eastern Mediterranean.

Bioinvasions constitute both a direct and an indirect threat to ecosystems. Direct threats include pressures on local trophic chains, while indirect threats might take the form of an invasion of a microorganism alongside its host. The marine dinoflagellate , parasitizing blue crabs (), has a worldwide distribution alongside its host.

Evidence for Beneficial Physiological Responses of the Land Snail to Probiotics' () Dietary Intervention.

A large variety of microorganisms ingested with food constitute animals' intestinal microflora, enhancing and maintaining the homeostasis of the host. Rations enriched with probiotics are a method recommended to prevent undesirable conditions. To date, research has been limited to farmed animals and reared fish, creating a knowledge gap concerning the effect of probiotics on the growth rate, physiological responses, and energy metabolism of invertebrates such as the land snail .

Heat hardening enhances metabolite-driven thermoprotection in the Mediterranean mussel .

Temperature affects organisms' metabolism and ecological performance. Owing to climate change, sea warming constituting a severe source of environmental stress for marine organisms, since it increases at alarming rates. Rapid warming can exceed resilience of marine organisms leading to fitness loss and mortality.

Association of MTNR1A and GDF9 gene allelles with the reproductive performance, response to oestrus induction treatments and prolificacy, in improved and non-improved local indigenous sheep breeds.

Sheep farming plays a crucial role for Mediterranean countries, wherefrom a plethora of dairy products is produced. Yet, numerous indigenous sheep breeds in temperate latitudes such as the Mediterranean present a serious drawback that milk production is impaired by the seasonality of reproduction. Towards the efforts for reduction of this phenomenon, the purpose of this study was to evaluate and compare different oestrus synchronization treatments, as well as to associate two genes alleles', namely the MTNR1A and the GDF9, with the response to those treatments.

The characterization of toll-like receptor repertoire in after mass mortality events suggests adaptive introgression.

The fan mussel is currently on the brink of extinction due to a multifactorial disease mainly caused to the highly pathogenic parasite , meaning that the selection pressure outweighs the adaptive potential of the species. Hopefully, rare individuals have been observed somehow resistant to the parasite, stretching the need to identify the traits underlying this better fitness. Among the candidate to explore at first intention are fast-evolving immune genes, of which toll-like receptor (TLR).

Multipathogen infections and multifactorial pathogenesis involved in noble pen shell () mass mortality events: Background and current pathologic approaches.

Disease outbreaks in several ecologically or commercially important invertebrate marine species have been reported in recent years all over the world. Mass mortality events (MMEs) have affected the noble pen shell (), causing its near extinction. Our knowledge of the dynamics of diseases affecting this species is still unclear.

High-Resolution Melting (HRM) Analysis for Rapid Molecular Identification of Species in the Greek Fish Market.

The red porgy () and the common dentex () are Sparidae species of high commercial value, traded in the Greek market. In some cases, fish species identification from Greek fisheries is difficult for the consumer due to the strong morphological similarities with their imported counterparts or closely related species such as , , and , especially when specimens are frozen, filleted or cooked. Techniques based on DNA sequencing, such as barcoding, accurately identify species substitution incidents; however, they are time consuming and expensive.

Probiotics, Prebiotics, and Synbiotics Utilization in Crayfish Aquaculture and Factors Affecting Gut Microbiota.

Aquaculture is affected by numerous factors that may cause various health threats that have to be controlled by the most environmentally friendly approaches. In this context, prebiotics, probiotics, and synbiotics are frequently incorporated into organisms' feeding rations to ameliorate the health status of the host's intestine, enhancing its functionality and physiological performance, and to confront increasing antimicrobial resistance. The first step in this direction is the understanding of the complex microbiome system of the organism in order to administer the optimal supplement, in the best concentration, and in the correct way.

Seasonality in Synergism with Multi-Pathogen Presence Leads to Mass Mortalities of the Highly Endangered in Greek Coastlines: A Pathophysiological Approach.

Mortalities of populations set at risk the survival of the species from many Mediterranean coastline habitats. In many cases, both and spp. are implicated in mass mortalities of populations, leading the species into extinction.

Development of Multiplex PCR and Melt-Curve Analysis for the Molecular Identification of Four Species of the Mullidae Family, Available in the Market.

The authentication of food products and the verification of their identity are of major importance for consumers. Food fraud through mislabeling is an illegal practice consisting of the substitution of an expensive food product by a relatively cheaper one, misleading false labelling of their origin and adulteration in processed or frozen products. This issue is particularly of high importance concerning fish and seafood, which are easily adulterated primarily due to difficult morphological identification.

Applicability evaluation of mtDNA based molecular identification in mosquito species/subspecies/biotypes collected from Thessaloniki, Greece.

The genus Culex, containing many described species, plays a role as a vector for diseases of medical and veterinary importance worldwide. Among these species, Culex pipiens is one of the most widespread mosquitoes and is classified into two biological forms (biotypes), named as Culex pipiens pipiens and Culex pipiens molestus. Due to similar morphological structure between these biotypes, morphological identification is inadequate.