Publications by authors named "Gianluigi Moro"
The imbalance between osteogenesis and adipogenesis, which naturally accompanies bone marrow senescence, may contribute to the development of bone-associated diseases, like osteoporosis. In the present study, using primary human mesenchymal stromal cells (hMSCs) isolated from trabecular bone, we assessed the possible effect of GH on hMSC differentiation potential into adipocytes. GH (5 ng/ml) significantly inhibited the lipid accumulation in hMSCs cultured for 14 days in lipogenic medium.
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- - Recent research shows that estrogens like 17β-estradiol (E2) influence growth hormone (GH) signaling in human osteoblasts, enhancing GH's effects on specific proteins and genes involved in bone formation.
- - E2 was found to significantly increase levels of phosphorylated STAT5 (P-STAT5) when added before GH treatment, suggesting a synergistic effect on bone-related gene expression such as osteopontin and bone-sialoprotein.
- - The study identifies SOCS2, a negative regulator of GH signaling, as a key player; E2 decreases SOCS2 protein levels through a non-genomic mechanism, promoting its degradation and thereby enhancing GH signaling efficacy in these bone cells
Transgenic mice over-expressing calcitonin gene-related peptide (CGRP) in osteoblasts have increased bone density due to increased bone formation, thus suggesting that CGRP plays a role in bone metabolism. In this study we determined the relationship between CGRP, the canonical Wnt signaling and apoptosis in human osteoblasts (hOBs) in consideration of the well-documented involvement of this pathway in bone cells. Primary cultures of hOBs were treated with CGRP 10(-8) M.
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