Synthetic redesign of Escherichia coli W for faster metabolism of sugarcane molasses.

Background: Sugarcane molasses, rich in sucrose, glucose, and fructose, offers a promising carbon source for industrial fermentation due to its abundance and low cost. However, challenges arise from the simultaneous utilization of multiple sugars and carbon catabolite repression (CCR). Despite its nutritional content, sucrose metabolism in Escherichia coli, except for W strain, remains poorly understood, hindering its use in microbial fermentation.

Synthetic biology tools for engineering .

is a promising organism for the industrial production of amino acids, fuels, and various value-added chemicals. From the whole genome sequence release, has been valuable in the field of industrial microbiology and biotechnology. Continuous discovery of genetic manipulations and regulation mechanisms has developed as a synthetic biology platform chassis.

Synthetic biology for evolutionary engineering: from perturbation of genotype to acquisition of desired phenotype.

With the increased attention on bio-based industry, demands for techniques that enable fast and effective strain improvement have been dramatically increased. Evolutionary engineering, which is less dependent on biological information, has been applied to strain improvement. Currently, synthetic biology has made great innovations in evolutionary engineering, particularly in the development of synthetic tools for phenotypic perturbation.