Engineering bi-layered skin-like nanopads with electrospun nanofibers and chitosan films for promoting fibroblast infiltration in tissue regeneration and wound healing.

This study presents an innovative bi-layered three-dimensional skin-like nanopad (SLN) engineered for skin tissue regeneration. The SLN integrates a mechanically supportive polycaprolactone nanofibrous layer with a functional chitosan hydrogel film, mimicking natural skin. Our SLN exhibits superior flexibility, with a maximum elongation of 751.

Bacteriophages and Their Clinical Applications.

Antimicrobial resistance poses a serious risk to contemporary healthcare since it reduces the number of bacterial illnesses that may be treated with antibiotics, particularly for patients with long-term conditions like cystic fibrosis (CF). People with a genetic predisposition to CF often have recurrent bacterial infections in their lungs due to a buildup of sticky mucus, necessitating long-term antibiotic treatment. infections are a major cause of CF lung illness, and airway isolates are frequently resistant to many antibiotics.

Bacteriophages and Green Synthesized Zinc Oxide Nanoparticles in Combination Are Efficient against Biofilm Formation of .

Bacteriophages (phages) are viruses that infect the bacteria within which their reproduction cycle takes place, a process that ends in the lysis and death of the bacterial cell. Some phages are also able to destroy bacterial biofilms. Due to increased antibiotics resistance, , another biofilm-forming pathogen, is a problem in many parts of the world.

N-Acetyl cysteine amide and cerium oxide nanoparticles as a drug delivery for ischemic stroke treatment: Inflammation and oxidative stress crosstalk.

Background: Inflammation and oxidative stress crosstalk is involved in the ischemic stroke(IS) pathogenesis and the new therapeutic options should be offered based on the targets that are critical in the golden hour of IS. YKL-40 and total antioxidant capacity(TAC), the inflammation and oxidative stress biomarkers, provide us with clues for proper intervention targets. N-acetyl cysteine amide (NACA), a lipophilic antioxidant, with a nanoparticle-based drug delivery system is permeable enough to penetrate blood-brain barrier (BBB) and was proposed as a new treatment option for IS.

Design of Phage-Cocktail-Containing Hydrogel for the Treatment of -Infected Wounds.

Recently, the treatment of infected wounds has become a global problem due to increased antibiotic resistance in bacteria. The Gram-negative opportunistic pathogen is often present in chronic skin infections, and it has become a threat to public health as it is increasingly multidrug resistant. Due to this, new measures to enable treatment of infections are necessary.

In vitro study of redroot pigweed effects as weed plant species on leukemia.

Redroot pigweed is a well-known allelopathic weed worldwide with diverse organic compounds which involving in its allelopathic interactions as well. Preliminary tests of redroot pigweed extract against leukemia and various human phatogenic microorganisms revealed that amaranth extract inhibits the viability and proliferation of NB4 cells in a time- and dose-dependent manner and has an excellent anti-bacterial effect on gram-positive bacteria and Candida fungi. Interestingly, the anti-luekemia effects of redroot pigweed is reported for the first time.

Combination of zeolite barrier and bio sparging techniques to enhance efficiency of organic hydrocarbon remediation in a model of shallow groundwater.

Adsorption and bioremediation are effective processes for remediation of benzene, toluene, and ethylbenzene (BTE) through Permeable Reactive Barriers (PRBs). A few researches focus on adsorption of natural zeolite because of its hydrophilic property. On the other hand, PRBs need to be replaced by fresh materials after a while when all the possible absorption positions were filled up.

UT1 Isolated from Northwest of Iran Traditional Curd Exerts Anti-proliferative and Apoptosis Inducing Effects in Human Colorectal Tumor HCT 116 Cells.

The present study was mainly designed to assess anti-cancer effects of lactobacilli isolated from traditional dairy products, on HCT116 colorectal cancer cell lines. Traditional dairy products samples were collected from the region of Azarbayjan and the suspensions were cultured in MRS agar medium. The isolates were identified by biochemical and molecular methods.

Design, synthesis and evaluation of biological activities of some novel anti-TB agents with bio-reducible functional group.

With regard to the anti-mycobacterial activity of 2-pyrazinoic acid esters (POEs), recent studies have shown that both pyrazine core and alkyl part of POE interact with the fatty acid synthase type (I) (FAS (I)) precluding a complex formation between NADPH and FAS (I). Considering this interaction at the reductase site of FAS (I) responsible for reduction of β-ketoacyl-CoA to β-hydroxyacyl-CoA, we hypothesized that POE containing a bioreducible center in its alkyl part might show an increased anti-tubercular activity due to the involvement of FAS (I) in extra bio-reduction reaction. Thus, we synthesized novel POEs, confirmed their structures by spectral data, and subsequently evaluated their anti-mycobacterial activity against (Mtb) (H37Rv) strain at 10 μg/mL concentration.

Identification of Probable Drug and Vaccine Candidates Against Antibiotic-Resistant .

is known as a Gram-negative bacterium that has become one of the most important health problems due to antibiotic resistance. Today, numerous efforts are being made to find new antibiotics against this nosocomial pathogen. As an alternative solution, finding bacterial target(s), necessary for survival and spread of most resistant strains, can be a benefit exploited in drug and vaccine design.

ZnO, AgCl and AgCl/ZnO nanocomposites incorporated chitosan in the form of hydrogel beads for photocatalytic degradation of MB, E. coli and S. aureus.

Significant improvement of effective and low-cost decolorization and disinfecting technologies is required to address the problems created by dyes and dangerous microorganisms from water and wastewaters. This article expresses the degradation of methylene blue (MB), Escherichia coli (E. coli) and Staphylococcus aureus (S.

Vancomycin Capped with Silver Nanoparticles as an Antibacterial Agent against Multi-Drug Resistance Bacteria.

Many antimicrobial medications are available to combat infections. However, the indiscriminate use of antibiotics has produced antibiotic resistance in the case of many bacterial pathogens. This study focuses on the development of nanoparticles (NPs) that enhance the in vitro antibiotic activity of vancomycin against multi-drug resistant (MDR) organisms.

Plasma surface modification as a new approach to protect urinary catheter against biofilm formation.

Background And Objectives: Biomaterials are widely used in medical devices such as urinary catheters. One of the main problems associated with long term using of the urinary catheters is biofilm formation on their surfaces. Many techniques have been presented to reduce the biofilm formation.

Novel functionalized monomers based on kojic acid: snythesis, characterization, polymerization and evalution of antimicrobial activity.

Two novel acrylate monomers, [5-(benzyloxy)-4-oxo-4-pyran-2-yl]methyl acrylate and {1-[(5-(benzyloxy)-4-oxo-4-pyran-2-yl)methyl]-1,2,3-triazol-4-yl}methyl acrylate were synthesized by the reaction of 5-benzyloxy-2-(hydroxymethyl)-4-pyran-4-one and 5-(benzyloxy)-2-{[4-(hydroxymethyl)-1,2,3-triazol-1-yl]methyl}-4-pyran-4-one with acryloyl chloride in the presence of triethylamine, respectively. These monomers were polymerized using 2,2-azobisisobutyronitrile (AIBN) as the initiator in ,-dimethylformamide:14-dioxane (10:1) solution. The thermal behavior of the polymers was investigated by thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC).

Effective Phages as Green Antimicrobial Agents Against Antibiotic-Resistant Hospital Escherichia coli.

Background: Bacteriophages are viruses that attack bacteria and lead to their lysis in an efficient and highly specific manner. These natural enemies of bacteria were used as therapeutic agents before the advent of antibiotics. Currently, with the rapid spread of multi-drug resistant bacteria, phage therapy can be an effective alternative treatment for antibiotic resistant bacteria.

Halotolerant Ability and α-Amylase Activity of Some Saltwater Fungal Isolates.

Four halotolerant fungal isolates originating from the saltwater Lake Urmia in Iran were selected during a screening program for salt resistance and α-amylase activity. The isolates were identified based on sequencing the ITS region and a part of the β-tubulin gene, as Penicillium chrysogenum (isolate U1; CBS 132820), Fusarium incarnatum (isolate U2; CBS 132821), and Penicillium polonicum (isolate U3; CBS 132822, and isolate U4; CBS 132823). The growth of these isolates was determined by measuring the colony diameter and mycelia dry weight in Sabouraud dextrose agar and yeast nitrogen base medium supplemented with NaCl, KCl, and LiCl.

Pyranocoumarins from Zosima absinthifolia (Vent) Link roots.

Zosima absinthifolia (Vent) Link (Apiaceae) is a perennial herb indigenous to Iran. It has been used as a medicinal plant from ancient time in Iran, Turkey and Pakistan. In the present work, air-dried and powdered plant roots were extracted with n-hexane, dichloromethane and methanol, respectively, using Soxhlet apparatus.

Biochemical characterization of an extracellular polyextremophilic α-amylase from the halophilic archaeon Halorubrum xinjiangense.

An extracellular haloalkaliphilic thermostable α-amylase producing archaeon was isolated from the saltwater Lake Urmia and identified as Halorubrum xinjiangense on the basis of morphological, biochemical, and molecular properties. The enzyme was purified to an electrophoretically homogenous state by 80 % cold ethanol precipitation, followed by affinity chromatography. The concentrated pure amylase was eluted as a single peak on fast protein liquid chromatography.

Bioactivity of malva sylvestris L., a medicinal plant from iran.

Objectives: Malva sylvestris L. (Malvaceae), an annual plant, has been already commonly used as a medicinal plant in Iran. In the present work, we evaluate some bioactivities of the plant extracts.

Isoarnottinin 4'-glucoside, a glycosylated coumarin from Prangos uloptera, with biological activity.

Coumarins are a well-known group of natural products distributed in the plant kingdom especially in the family Apiaceae with various biological activities. Isoarnottinin 4'-glucoside is a simple glycosylated coumarin found previously in a few genera of Apiaceae, and its biological activities have not been previously described in details. In the present paper, the compound was isolated from Prangos uloptera (Apiaceae) leaves using HPLC techniques.

Post-antibacterial effect of thymol.

The antibacterial activity of thymol has been well established and reported in the scientific literature. Continued suppression of bacterial growth following limited exposure to antimicrobial compounds at different concentrations greater than or equal to the minimum inhibitory concentration level (MIC) and at concentrations less than the MIC can be used as an indicator of biological activity, and are respectively referred to as a post-antibacterial effect (PAE) and a post-antibiotic sub-MIC effect (PA-SME). In this study, the PAE and the PA-SME of thymol against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus cereus were investigated.

Bioactivity of aviprin and aviprin-3''-O-glucoside, two linear furanocoumarins from Apiaceae.

Furanocoumarins are well-known natural products that occur in the most evolved genera of Apiaceae family. This compounds were found to have cytotoxic, phytotoxic, photosensitizing, insecticidal, antibacterial and high anti-fungal effects. Aviprin is considered as a linear furanocoumarin substituted at C8 with an oxygenated prenyl residue.

A furanocoumarin from Prangos uloptera roots, biological effects.

A known furanocoumarin, 8-geranyloxy psoralen, was isolated from n-hexane extract of Prangos uloptera roots by the TLC method. Its structure was determined by comparison of the spectral data with the literature. Cytotoxic effects of the isolated compound were determined by MTT and Tripan blue assays.

Biological activity of quercetin-3-O-glucoside, a known plant flavonoid.

Cytotoxic, phytotoxic, antimicrobial and antioxidant effects of quercetin 3-O-glucoside (Q3G) isolated by HPLC from aerial parts of Prangos ferulaceae was studied by MTT assay, lettuce germination assay, disk diffusion and DPPH method. Our results showed that Q3G exhibits high antioxidant effect with RC(50) of 22 microg/mL, it has low cytotoxicity and no antibacterial effects. Q3G exhibits high phytotoxic effect with IC(50) value of 282.

Biological activity of Prangos uloptera DC. roots, a medicinal plant from Iran.

Prangos uloptera DC. (Apiaceae) is indigenous to Iran. It has been used from ancient times for treatment of leukoplakia, digestive disorders and healing scars.