Aframomumlabdane, a new bislabdane diterpenoid from zingiberaceae).

A previously undescribed bislabdane diterpenoid namely aframomumlabdane (), was isolated from the seed of together with seven known compounds (-). Their structures were established based on a comprehensive analysis of HR-ESI-MS, in conjunction with their 1D and 2D-NMR data. Compound was evaluated for its cytotoxic activity against four cancer cell lines: A549, HepG2, SPC212 and DLD-1.

Liver Injury in COVID-19 Infection: A Systematic Review.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) or novel coronavirus disease (COVID-19) pandemic is sweeping through the world. The overwhelming pathology seems to be in the upper and lower respiratory tract; however, the involvement of other organs, including the liver, has also been reported. Whether liver enzyme derangement is a common feature of COVID-19 is not known.

Cholestasis of Sepsis: A Case Report.

A 46-year-old man presented with fever, general lethargy, and weight loss over the last few months. He started to develop jaundice and his condition worsened. Blood tests confirmed rising levels of conjugated bilirubin with near-normal alanine aminotransferase, alkaline phosphatase, and prothrombin time.

Phosphorylcholine-endcapped oligomer and block co-oligomer and surface biological reactivity.

Phosphorylcholine (PC)-endcapped oligomer and block co-oligomer were prepared by employing a photoiniferter-based quasi-living polymerization technique. The designed oligomer had a PC polar head group attached to an alkylene chain at one end of the molecule and an oligo(styrene) (oligoST) segment at the other end. In the co-oligomer, an oligo(N,N-dimethylacrylamide) (oligoDMAAm) segment was inserted between both ends of the oligomer mentioned above.

Genomic DNA analysis of rodent mycoplasmas.

Genomic DNA was compared between three typical species of rodent mycoplasmas, Mycoplasma pulmonis, M. arthritidis and M. neurolyticum, and between strains of these species.

A DNA probe for specific detection of Mycoplasma pulmonis.

Mycoplasma pulmonis was specifically detected by using a 2.3 kilobase pair (kbp) cloned DNA fragment derived from M. pulmonis m 53 as a probe.

Expression of Mycoplasma pulmonis antigens in Escherichia coli.

The expression of Mycoplasma pulmonis antigen in Escherichia coli was investigated by cloning genomic DNA derived from M. pulmonis m 53, and the DNA fragment participating in antigen expression was identified. When the DNA library of M.

Cross reactivity of Mycoplasma pulmonis and Mycoplasma arthritidis antigen strains to anti-Mycoplasma pulmonis antibody in the sera of Mycoplasma pulmonis infected rats.

Reactivity of Mycoplasma pulmonis (Mp) antigen strains to anti-Mp antibody in the sera of Mp infected rats was examined by enzyme linked immunosorbent assay (ELISA). Antibody titers to 7 kinds of Mp antigens were measured in the sera of 20 Mp isolated rats and 20 Mp free rats by ELISA and complement fixation test (CF test). ELISA showed that there was no difference in the antibody titer of the same serum among 7 Mp antigen strains employed, and the main cross reaction to anti-Mp antibody took place on the common recognition site (common antigen) in all the Mp antigens.

Interstitial pneumonitis in autoimmune MRL/lpr mice and its treatment with cyclosporin A.

Age-associated changes of anti-double-stranded (ds) DNA antibodies, anti-single-stranded (ss) DNA antibodies, and serum immune complex concentrations were studied in MRL/lpr mice. All anti-ds DNA antibodies, anti-ss DNA antibodies, and immune complexes began to be detected in the sera of MRL/lpr mice aged 8 to 13 weeks and increased remarkably after 17 weeks of age. Almost no pathological findings were observed histologically in the lungs of MRL/lpr mice aged 8 weeks but interstitial pneumonitis became evident at 14 weeks of age.

A solid-phase enzyme immunoassay for anti-insulin antibody in diabetes mellitus patients.

A solid-phase enzyme immunoassay for the measurement of anti-insulin antibodies in the sera of patients with diabetes mellitus was developed. Porcine insulin conjugated with bovine serum albumin was used for coating microtiter plates. This assay was as sensitive as the conventional radioimmunoassay.

A solid-phase enzyme immunoassay for anti-acetylcholine receptor antibody in myasthenia gravis patients.

A solid-phase enzyme immunoassay for the measurement of anti-acetylcholine receptor antibodies in the sera of patients with myasthenia gravis is reported. Sufficient amounts of acetylcholine receptor for the sensitive detection of anti-acetylcholine receptor antibody were directly fixed to Costar serocluster 96-well EIA plates coated with poly-L-lysine hydrobromide. The solid-phase enzyme immunoassay detected anti-acetylcholine receptor antibodies in 91% of the myasthenia gravis patients including 4 out of 4 ocular type myasthenia patients, anti-acetylcholine receptor antibodies of which were not detectable by the immunoprecipitation assay.

Age-associated changes of the characteristics of autoantibody-forming cells in NZB/W mice.

Age-associated change of characteristics of anti-double-stranded (ds) DNA antibody-forming cells in the NZB/W mouse spleen was studied. These cells in mice aged 9-11 months, which develop high titer anti-ds DNA antibody formation, produced antibody without the help of T cells or pokeweed mitogen (PWM), adhered to Sephadex G-10, and sedimented to the bottom when applied to Ficoll-Paque solution. On the other hand, these cells in relatively young (7- to 8-month-old) mice, which develop low titer anti-ds DNA antibody formation, produced antibody maximally with the help of T cells and PWM, adhered little to Sephadex G-10, and fractionated into intermediate lymphocyte fractions when applied to Ficoll-Paque solution.

Characterization of anti-double-stranded DNA antibody-forming cells in the NZB/W mouse spleen.

Characterization of anti-double-stranded (ds) DNA antibody-forming cells in the spleen of the NZB/W mouse was attempted. Anti-ds DNA antibody-forming cells formed antibodies in vitro without any antigenic stimulus. Depletion of T cells in the NZB/W spleen cells did not abolish but enriched the anti-ds DNA antibody-forming capacity.

Common protective antigen between Pseudomanas aeruginosa and Vibrio cholerae.

Immunization with a common antigen (OEP) of Pseudomonas aeruginosa produced a high level of protection in mice against Vibrio cholerae infection. The average OEP-HA titer in mice sera of immune group was 1,600 HA titer. However, vibriocidal titer and agglutinin titer against V.