Activation studies of the β-carbonic anhydrases from with amines and amino acids.

The β-carbonic anhydrase (CA, EC 4.2.1.

Sulfonamide Inhibition Profile of the β-Carbonic Anhydrase from , An Opportunistic Pathogen Triggering Scalp Conditions.

The critical CO hydration reaction to bicarbonate and protons is catalyzed by carbonic anhydrases (CAs, EC 4.2.1.

Anion Inhibition Profile of the β-Carbonic Anhydrase from the Opportunist Pathogenic Fungus Involved in Dandruff and Seborrheic Dermatitis.

Carbonic anhydrases (CAs, EC 4.2.1.

Cloning, Purification, and Characterization of a β-Carbonic Anhydrase from , an Opportunistic Pathogen Involved in Dandruff and Seborrheic Dermatitis.

The cloning, purification, and initial characterization of the β-carbonic anhydrase (CA, EC 4.2.1.

The Evaluation of The Real Alpha Value in Brazil and its Projection until The Year 2050.

When a cost-benefit analysis is applied to the optimization of practices involving radiation protection, the alpha value is used to determine the amount of money required to be invested in a practice to minimize radiation doses to acceptable levels. The alpha value is often linked to the gross domestic product (GDP) per capita, so the monetary reference value of person-Sievert can often be different in each country. Evaluation of the alpha value in Brazil was performed in 1993 and 2000 making use of the procedure advised by ICRP to produce projections up to 2015 and subsequently in 2004 by using the procedure recommended by the IAEA.

On-Demand Dissolution of a Dendritic Hydrogel-based Dressing for Second-Degree Burn Wounds through Thiol-Thioester Exchange Reaction.

An adhesive yet easily removable burn wound dressing represents a breakthrough in second-degree burn wound care. Current second-degree burn wound dressings absorb wound exudate, reduce bacterial infections, and maintain a moist environment for healing, but are surgically or mechanically debrided from the wound, causing additional trauma to the newly formed tissues. We have developed an on-demand dissolvable dendritic thioester hydrogel burn dressing for second-degree burn care.

Recent Advances in Dendritic Macromonomers for Hydrogel Formation and Their Medical Applications.

Hydrogels represent one of the most important classes of biomaterials and are of interest for various medical applications including wound repair, tissue engineering, and drug release. Hydrogels possess tunable mechanical properties, biocompatibility, nontoxicity, and similarity to natural soft tissues. The need for hydrogels with specific properties, based on the design requirements of the in vitro, in vivo, or clinical application, motivates researchers to develop new synthetic approaches and cross-linking methodologies to form novel hydrogels with unique properties.

Synthetic Biomaterials from Metabolically Derived Synthons.

The utility of metabolic synthons as the building blocks for new biomaterials is based on the early application and success of hydroxy acid based polyesters as degradable sutures and controlled drug delivery matrices. The sheer number of potential monomers derived from the metabolome (e.g.

Development and applications of a DNA labeling method with magnetic nanoparticles to study the role of horizontal gene transfer events between bacteria in soil pollutant bioremediation processes.

Horizontal gene transfers are critical mechanisms of bacterial evolution and adaptation that are involved to a significant level in the degradation of toxic molecules such as xenobiotic pesticides. However, understanding how these mechanisms are regulated in situ and how they could be used by man to increase the degradation potential of soil microbes is compromised by conceptual and technical limitations. This includes the physical and chemical complexity and heterogeneity in such environments leading to an extreme bacterial taxonomical diversity and a strong redundancy of genes and functions.

The efficacy of a lysine-based dendritic hydrogel does not differ from those of commercially available tissue sealants and adhesives: an ex vivo study.

Background: Hemostatic agents, tissue adhesives and sealants may contribute to a reduction in hemorrhage-associated morbidity and mortality. Towards this end, we have recently developed a lysine-based dendritic hydrogel (PEG-LysNH2) that can potentially be used in the management of severe trauma and/or intraoperative bleeding. As a first step in demonstrating the potential utility of this approach, our objective was to ascertain the ability of the PEG-LysNH2 to adhere to and seal injured tissues, as well as to maintain the seal under physiological conditions.

The chemistry and engineering of polymeric hydrogel adhesives for wound closure: a tutorial.

The closure and repair of wounds after traumatic or surgical injury is of significant clinical and research importance. While sutures remain the common wound closure technique, they have many disadvantages. Consequently, polymeric hydrogel adhesives have emerged as essential materials for wound management and repair because of their tunable chemical and physical properties, which enable them to adhere or stick to tissues, possess sufficient mechanical strength to stay intact and be subsequently removed, provide complete wound occlusion, and act as a barrier to bacterial infection.

Synthesis of Hydrophobic Carbohydrate Polymers and Their Formation of Thermotropic Liquid Crystalline Phases.

The first synthesis of enantiopure glucose octyl ether polyamido-saccharides (GOE-PAS) with a defined molecular weight and narrow dispersity is reported using a controlled anionic ring-opening polymerization of a glucose-derived β-lactam sugar monomer possessing octyl ether chains. This new polymer structure is characterized by NMR, infrared (IR), optical rotation, gel permeation chromatography (GPC), and thermogravimetric analysis (TGA). At room temperature, the polymers form lamellar (Lam) phases.

A dendritic thioester hydrogel based on thiol-thioester exchange as a dissolvable sealant system for wound closure.

A dissolvable dendritic thioester hydrogel based on thiol-thioester exchange for wound closure is reported. The hydrogel sealant adheres strongly to tissues, closes an vein puncture, and withstands high pressures placed on a wound. The hydrogel sealant can be completely washed off upon exposure to thiolates based on thiol-thioester exchange and allow gradual wound re-exposure during definitive surgical care.

A bisphosphonate tweezers and clickable PEGylated PAMAM dendrons for the preparation of functional iron oxide nanoparticles displaying renal and hepatobiliary elimination.

The functionalization of superparamagnetic iron oxide nanoparticles (SPION) with PEGylated PAMAM dendrons through a bisphosphonate tweezers yielded 15 and 30 nm dendritic nano-objects stable in physiological media and showing both renal and hepatobiliary elimination.

Effect of the nanoparticle synthesis method on dendronized iron oxides as MRI contrast agents.

Aqueous suspensions of dendronized iron oxide nanoparticles (NPs) have been obtained after functionalization, with two types of dendrons, of NPs synthesized either by coprecipitation (leading to naked NPs in water) or by thermal decomposition (NPs in situ coated by oleic acid in an organic solvent). Different grafting strategies have been optimized depending on the NPs synthetic method. The size distribution, the colloidal stability in isoosmolar media, the surface complex nature as well as the preliminary biokinetic studies performed with optical imaging, and the contrast enhancement properties evaluated through in vitro and in vivo MRI experiments, have been compared as a function of the nature of both dendrons and NPs.

Dendronized iron oxide nanoparticles for multimodal imaging.

The synthesis of small-size dendrons and their grafting at the surface of iron oxide nanoparticles were achieved with the double objective to obtain a good colloidal stability with a mean hydrodynamic diameter smaller than 100 nm and to ensure the possibility of tuning the organic coating characteristics including morphology, functionalities, physico-chemical properties, grafting of fluorescent or targeting molecules. Magnetic resonance and fluorescence imaging are then demonstrated to be simultaneously possible using such versatile superparamagnetic iron oxide nanocrystals covered by a dendritic shell displaying either carboxylate or ammonium groups at their periphery which could be further labelled with a fluorescent dye. The grafting conditions of these functionalized dendrons at the surface of SPIO NPs synthesized by co-precipitation have been optimized as a function of the nature of the peripheral functional group.

Theoretical mechanistic study of the TBD-catalyzed intramolecular aldol reaction of ketoaldehydes.

The intramolecular aldol reaction of acyclic ketoaldehydes catalyzed by 1,5,7-triazabicyclo[4.4.0]dec-5-ene (TBD) is investigated using density functional theory calculations.