The Effect of Propolis on Hatched Eggs Previously Infected With Avian Pathogenic E. coli.

We investigated the effect of propolis as a sanitiser on hatched eggs previously infected with avian Pathogenic Escherichia coli (E. coli) (APEC) serogroup O78. A green propolis watery extract at 24% and a native breed hatching eggs have been used in this study.

Chicken -defensin-1 peptide as a candidate anticoccidial agent in broiler chickens.

The current study aimed to investigate the potentiality of using avian β-defensin-1 peptide as a candidate agent against coccidiosis infection in broiler chicken.We employed an in-silico analysis to study the primary structure of β-defensin-1 peptide as well as its 3-D and molecular dynamic structures. This will also enable obtaining adequate information about the mode of action of these peptides and the intra-cellular transduction pathways.

Infectious Bronchitis Virus Genotypes in the Middle East.

Infectious bronchitis virus (IBV) variability is the result of genetic mutations and/or recombination events followed by selection. Different IBV types have been reported in Middle Eastern countries, of which most relate to different lineages of genotype I based on a phylogenetic analysis of the full-length S1 subunit of the spike gene. Despite extensive vaccination programs, IBV variants continue to emerge in the Middle East.

Molecular characterization of some isolates of rabbit viral hemorrhagic disease (VHD) in Egypt from 2014 to 2019.

Objective: Rabbit viral hemorrhagic disease (VHD) is a transmittable and lethal viral illness of rabbits. In this study, genetic identification and genetic analysis of the rabbit hemorrhagic disease virus (RHDV) was made in three governorates in Egypt from 2014 to 2019.

Materials And Methods: Livers from 18 freshly dead rabbits, which was guessed to be VHD epidemics in Egypt (Giza, Menofia, and Fayoum governorates) from 2014 to 2019, were examined for RHDV.

ISOLATION AND MOLECULAR IDENTIFICATION OF IBV ISOLATES IN DIFFERENT GOVERNORATES IN EGYPT.

Tracheal swabs and different organs are collected from 17 chicken farms showing respiratory signs and variable mortalities in different governorates. Three successive blind serial passages were performed. Four IBV isolates are detected in vaccinated chickens by RT-PCR and are identified by sequence and phylogenetic analysis of portion of Si gene.

Kidney Cell-Adapted Infectious Bronchitis Virus Arkansas Delmarva Poultry Industry Vaccine Confers Effective Protection Against Challenge.

We previously demonstrated that adaptation of an embryo-attenuated infectious bronchitis virus (IBV) Arkansas Delmarva Poultry Industry (ArkDPI)-derived vaccine to chicken embryo kidney (CEK) cells shifted the virus population towards homogeneity in spike (S) and nonstructural protein genes. Moreover, the typical Ark vaccine subpopulations emerging in chickens vaccinated with commercial Ark vaccines were not detected in chickens vaccinated with the CEK-adapted virus. In this study, chickens vaccinated with a low dose (1.

Cross-Protection by Infectious Bronchitis Viruses Under Controlled Experimental Conditions.

Infectious bronchitis virus (IBV) cross-protection trials were performed in healthy chickens maintained under controlled environmental conditions. Chickens primed or primed and boosted with a Massachusetts (Mass)-type attenuated vaccine were subsequently challenged with either IBV Arkansas (Ark) or GA13-type virulent strains. In addition, Ark-vaccinated chickens were challenged with IBV GA13.

Effects of Adaptation of Infectious Bronchitis Virus Arkansas Attenuated Vaccine to Embryonic Kidney Cells.

The population structure of an embryo-attenuated infectious bronchitis virus (IBV) Arkansas (Ark) Delmarva Poultry Industry (DPI)-derived vaccine was characterized during serial passages in chicken embryo kidney (CEK) cells and after back-passage in embryonated chicken eggs (ECE) and in chickens. Both conventional and deep-sequencing results consistently showed population changes occurred during adaptation to CEK cells. Specifically, 13 amino acid (aa) positions seemed to be targets of selection when comparing the vaccine genome prior to and after seven passages in CEK (CEKp7).