In vitro regeneration approaches for restoration of Ceropegia mohanramii-an endemic and critically endangered asclepiad.

The study aimed to develop an efficient, rapid, and large-scale in vitro regeneration system for propagation, conservation, and restoration of an endemic and critically endangered herb, Ceropegia mohanramii. The cultures were established using nodal explants on Murashige and Skoog's (MS) medium supplemented with 6-benzylaminopurine (BAP: 1.0 mg/l).

Highly efficient regeneration, establishment of callus and cell suspension cultures and RAPD analysis of regenerants of Burkill.

Highly efficient regeneration system has been developed for Burkill, an important herb used as substitute for . Shoot tips explants were cultured on MS medium with various phytohormones for multiple shoot production. The best shoot production frequency (100%) and maximum shoots (10.

Seasonal variation in content of camptothecin from the bark of Nothapodytes nimmoniana (Grah.) Mabb., using HPLC analysis.

Objective: To study and compare seasonal variation in camptothecin (CPT) content from bark samples of Nothapodytes nimmoniana obtained from geographically and climatologically isolated populations.

Methods: A standard High Performance Liquid Chromatography methodology was used to analyze and quantify CPT from bark samples of N. nimmoniana.

Free amino acid profiling in grain amaranth using LC-MS/MS.

Reliable and reproducible techniques for identification and quantification of amino acids usually require derivatization. However, techniques such as LC-MS/MS may perhaps sideline the derivatization with significant accuracy. Understanding the interaction between free amino acids and several physiological processes apparently requires that the pool be studied in the seed itself.