SERS profiling of blood serum filtrate components from patients with type II diabetes using 100 kDa filtration devices.

Blood carries some of the most valuable biomarkers for disease screening as it interacts with various tissues and organs in the body. Human blood serum is a reservoir of high molecular weight fraction (HMWF) and low molecular weight fraction (LMWF) proteins. The LMWF proteins are considered disease marker proteins and are often suppressed by HMWF proteins during analysis.

A Cross-Sectional Serological Study to Assess the Prevalence and Risk Factors of Anaplasmosis in Dromedary Camels in Punjab, Pakistan.

Anaplasmosis is an infectious disease transmitted by ticks and caused by obligate intracellular pathogen of belonging to genus Infections of one-humped camels () and llamas () have been reported previously. The aim of this study was to investigate the seroprevalence and risk factors of anti- spp. antibodies in of the Punjab, Pakistan.

Tumor Budding, Poorly Differentiated Clusters, and Stroma Percentage in Uterine Endometrioid Carcinomas Are Morphologic Findings Associated With Adverse Clinical Outcomes.

Uterine endometrioid carcinoma (UEC) has well-defined morphologic features that carry prognostic significance and guide treatment. In addition to the well-known features, tumor budding (TB), poorly differentiated clusters (PDCs), and amount of stroma within the invasive front are associated with a poor prognostic outcome in many carcinomas. Here, we evaluate TB, PDCs, and the stroma percentage in UEC and correlate these findings with morphologic features known to be associated with a poor clinical outcome and with recurrence-free survival (RFS).

Laccase purification and azo dye decolorization potential of .

often referred to as Cauliflower mushroom possess both medicinal and edibility values. In this research work, first time laccase purification and dye colourisation efficacy of 's purified laccase were assessed. Optimal laccase potential was noted after 12 day of incubation with 4 pH of medium at 45 °C.

EFNet: A multitask deep learning network for simultaneous quantification of left ventricle structure and function.

Purpose: The purpose of this study is to develop an automated method using deep learning for the reliable and precise quantification of left ventricle structure and function from echocardiogram videos, eliminating the need to identify end-systolic and end-diastolic frames. This addresses the variability and potential inaccuracies associated with manual quantification, aiming to improve the diagnosis and management of cardiovascular conditions.

Methods: A single, fully automated multitask network, the EchoFused Network (EFNet) is introduced that simultaneously addresses both left ventricle segmentation and ejection fraction estimation tasks through cross-module fusion.

Optimum study of fractional polio model with exponential decay kernel.

This study introduces a fractional order model to investigate the dynamics of polio disease spread, focusing on its significance, unique results, and conclusions. We emphasize the importance of understanding polio transmission dynamics and propose a novel approach using a fractional order model with an exponential decay kernel. Through rigorous analysis, including existence and stability assessment applying the Caputo Fabrizio fractional operator, we derive key insights into the disease dynamics.

DeepARV: ensemble deep learning to predict drug-drug interaction of clinical relevance with antiretroviral therapy.

Drug-drug interaction (DDI) may result in clinical toxicity or treatment failure of antiretroviral therapy (ARV) or comedications. Despite the high number of possible drug combinations, only a limited number of clinical DDI studies are conducted. Computational prediction of DDIs could provide key evidence for the rational management of complex therapies.

Optimizing lung cancer classification through hyperparameter tuning.

Artificial intelligence is steadily permeating various sectors, including healthcare. This research specifically addresses lung cancer, the world's deadliest disease with the highest mortality rate. Two primary factors contribute to its onset: genetic predisposition and environmental factors, such as smoking and exposure to pollutants.

Self-Assembly of Peptides as an Alluring Approach toward Cancer Treatment and Imaging.

Cancer is a severe threat to humans, as it is the second leading cause of death after cardiovascular diseases and still poses the biggest challenge in the world of medicine. Due to its higher mortality rates and resistance, it requires a more focused and productive approach to provide the solution for it. Many therapies promising to deliver favorable results, such as chemotherapy and radiotherapy, have come up with more negatives than positives.

and antiproliferative potential of isolated flavonoids constitutes from .

Cancer is one of the most demanding domains for innovative, effective, safe, and affordable therapeutically active chemicals. The main aim of this study is to research new phytochemicals with anticancer activity. The current experiment identified and analyzed six compounds for anti-cancer potential supported by molecular simulation studies.

Mix design process for sustainable self-compacting geopolymer concrete.

Faster growth of infrastructure emphasizes the utilization of eco-friendly construction materials with a low carbon footprint. The objective of this experimental study is to propose a mix design methodology for self-compacting geopolymer concrete (SCGC) considering various influencing parameters recommended for self-compacting concrete (SCC) and geopolymer concrete (GPC). The experimental test results depict that SCGC had similar flow deformability with comparatively lesser flow speed compared to SCC.

Spectrum Of HBB Gene Variants And Major Endocrine Complications In Thalassemia Patients Of Pakistan.

Objective: To determine the molecular characterisation of beta-thalassemia major patients, pattern of major endocrine complications and its association with haemoglobin subunit beta gene variants.

Method: The cross-sectional study was conducted from November 2021 to November 2022 after approval from the ethics review committee of Liaquat University of Medical and Health Sciences, Jamshoro, Pakistan, and comprised of 88 patients with beta thalassemia major aged >8 years and having serum ferritin level >1000 μg/L. The subjects were analysed for haemoglobin subunit beta gene variants and major endocrine complications, like growth retardation, hypogonadism, hypothyroidism, hypoparathyroidism and diabetic abnormalities using an automatic chemistry analyser, fully automatic chemiluminescence immunoassay analyser, enzyme-linked immunosorbent assay and real-time polymerase chain reaction.

Ejection Fraction Estimation from Echocardiograms Using Optimal Left Ventricle Feature Extraction Based on Clinical Methods.

Echocardiography is one of the imaging systems most often utilized for assessing heart anatomy and function. Left ventricle ejection fraction (LVEF) is an important clinical variable assessed from echocardiography via the measurement of left ventricle (LV) parameters. Significant inter-observer and intra-observer variability is seen when LVEF is quantified by cardiologists using huge echocardiography data.

Revealing novel and 5 genes-based population diversity and benzimidazole resistance in of bovine origin.

Cystic echinococcosis (CE) is a neglected zoonotic disease caused by (). The parasite affects a wide range of livestock and wild animals. In this study, the population diversity of the species was investigated based on mitochondrial cytochrome b (b) and NADH dehydrogenase subunit 5 (5) genes.

Higher versus lower blood pressure targets after cardiac arrest: A meta-analysis of randomized controlled trials.

A few mostly underpowered randomized controlled trials (RCTs) have been used to study the impact of blood pressure (BP) targets in out-of-hospital cardiac arrest (OHCA) patients. We aimed to perform an updated meta-analysis to compare the outcomes between the higher BP target and the lower BP target groups following OHCA. A systematic search was conducted on PubMed, Embase and the Cochrane Library until December 2022.

Dental aesthetic related popularity and peer pressure, a survey of adolescents in Pakistan.

Objectives: Most research has focused on determining how popularity and peer pressure impact behaviours, rather than identifying a key feature such as dental aesthetics and studying its impacts in relation to popularity and peer pressure.

Methods: A cross sectional study was conducted on a sample of 527 children from four schools located in Lahore, Pakistan. A 14-point questionnaire was developed, using existing measures of peer pressure, and popularity.

In-situ synthesized ZIF-67 graphene oxide (ZIF-67/GO) nanocomposite for efficient individual and simultaneous detection of heavy metal ions.

Heavy metals are ubiquitous in water bodies as a result of anthropogenic activities and over time they accumulate in body thus posing serious health problems. Therefore, it is essential to improve sensing performance, for determination of heavy metal ions (HMIs), of electrochemical sensors. In this work, cobalt-derived MOF (ZIF-67) was in-situ synthesized and incorporated onto the surface of graphene oxide (GO) by simple sonication method.

Curcumin for the treatment of COVID-19 patients: A meta-analysis of randomized controlled trials.

Curcumin is a low-cost and easily accessible therapeutic option for COVID-19 patients. We aimed to conduct a meta-analysis to assess the effect of curcumin on clinical outcomes in COVID-19 patients. Various databases, including PubMed, the Cochrane Library and Embase were searched from inception until October 2022 for randomized controlled trials (RCTs) evaluating curcumin use in COVID-19 patients.

Phylogeny and population structure of Echinococcus granulosus (sensu stricto) based on full-length cytb-nad2-atp6 mitochondrial genes - First report from Sialkot District of Pakistan.

Cystic echinococcosis is a zoonotic disease of livestock having serious economic setbacks. The etiological agents of the disease belong to Echinococcus granulosus sensu lato. Despite of worldwide distribution of the disease, the molecular studies mainly employ amplification of cox1, nad1 and nad5 genes.

Enhancing activity of β-lactam and fluoroquinolones antibiotics by artemisinin and its derivatives against MDR .

Artemisinin and its derivatives had played a biocidal role in biomedical remedies, while they were expected to enhance the activity of antibiotics against multiple drug-resistant (MDR) bacteria. The current study evaluated the interaction of artemisinin (ART), dihydroartemisinin (DHA), artesunate (AS), and artemisinic acid (AA) with β-lactam and fluoroquinolones antibiotics against . Antibiotic strip test (-test), Kirby Bauer's disc test (KB method), and broth microdilution method were adopted for susceptibility analysis, while the checkerboard method was applied to assess synergisms.

The effects of surface inclination on gastrocnemius, soleus and tibialis anterior muscle activation during gait.

Background: Inclined walking is associated with multiple musculoskeletal benefits and is considered a therapeutic exercise. Various patterns of increased and decreased muscle activation with inclined surfaces have been observed in normal muscles, with more focus on the proximal lower limb musculature.

Objective: The aim of this study was to assess the differences in electromyographic activation of gastrocnemius, soleus, and tibialis anterior at various inclined surfaces during gait.

Seroprevalence and Molecular Evidence of in Dromedary Camels of Pakistan.

Coxiellosis is a zoonosis in animals caused by . A cross-sectional study was conducted on 920 (591 female and 329 male) randomly selected camels () of different age groups from 13 districts representative of the three different ecological zones in the Province Punjab, Pakistan to determine the prevalence and associated risk factors of coxiellosis. The blood samples were collected and tested for anti- antibodies using indirect multispecies ELISA.