Draft Genome Sequence of Plant Growth-Promoting Bacillus altitudinis Strain PAE4.

We report here the draft genome of Bacillus altitudinis strain PAE4, a thermophilic plant growth-promoting rhizobacterium isolated from the coastal ridge of the Mediterranean Sea in Egypt. Besides heat shock protein genes, several genes encoding phytobeneficial properties were identified.

Expression of synthetic human tropoelastin (hTE) protein in Nicotiana tabacum.

Plant molecular farming (PMF) is an important growing prospective approach in plant biotechnology; it includes production of recombinant pharmaceutical and industrial proteins in large quantities from engineered plants. Elastin is a major protein component of tissues that require elasticity, it helps keep skin smooth as it stretches to allow normal. Elastin is used as a raw material for the cosmetic industry.

Impact of cis-acting elements' frequency in transcription activity in dicot and monocot plants.

The production of new cultivars via recombinant DNA technology is important in applied agriculture. Promoters play fundamental roles in successful transformation and gene expression. Fragments of the upstream regulatory region of the movement protein gene of the Tomato yellow leaf curl virus (TYLCV; two fragments) and Watermelon chlorotic stunt virus (WmCSV, two fragments) and one fragment of the coat protein putative promoter of TYLCV (CPTY-pro) were isolated to assess their abilities to drive expression in monocot and dicot plants.

Optimization of transient gene expression system in Gerbera jemosonii petals.

Low transformation efficiency and long generation time for production of transgenic Gerbera jemosonii plants leads to vulnerable gene function studies. Thus, transient expression of genes would be an efficient alternative. In this investigation, a transient expression system for gerbera petals based on the Agrobacterium infiltration protocol was developed using the reporter genes β-glucuronidase (gus) and green florescence protein (gfp).

Effect of growth hormones on some antioxidant parameters and gene expression in tomato.

Bioregulators have a great effect on vital processes of plant growth and development. Known plant bioregulators include Naphthalene acetic acid (NAA), Indole-3-butyric acid (IBA) and Indole-3-acetic acid (IAA). Natural or synthetic plant bioregulators are organic compounds that affect the physiological processes in the plant, either to control some of these processes or to modify them.

MERE1, a low-copy-number copia-type retroelement in Medicago truncatula active during tissue culture.

We have identified an active Medicago truncatula copia-like retroelement called Medicago RetroElement1-1 (MERE1-1) as an insertion in the symbiotic NSP2 gene. MERE1-1 belongs to a low-copy-number family in the sequenced Medicago genome. These copies are highly related, but only three of them have a complete coding region and polymorphism exists between the long terminal repeats of these different copies.