Toxinotyping of strains isolated from broiler flocks with necrotic enteritis and evaluation of the effect of toxins on Leghorn Male Hepatoma cells.

Background: Necrotic enteritis (NE) is an economically important disease, caused by type G strains, and is one of the major targets of antibiotics used in poultry feed.

Aims: This study aimed to genotypically characterize virulent strains of isolated from healthy and diseased birds in Iran.

Methods: Eleven isolates were derived from necrotic enteritis cases, and 27 were from healthy chickens.