Developmental and Cell Fate Analyses Support a Postnatal Origin for the Cortical Collecting System in the Mouse Kidney.

Background: Structure and function in the mammalian kidney are organized along a radial axis highlighted by the corticomedullary organization and regional patterning of the collecting system. The arborised collecting epithelium arises through controlled growth, branching and commitment of Wnt11+ ureteric progenitor cells within cortically localized branch tips until postnatal day 3.

Methods: We applied in situ hybridization and immunofluorescence to key markers of collecting duct cell types to examine their distribution in the embryonic and postnatal mouse kidneys.

Graft incompatibility between pepper and tomato elicits an immune response and triggers localized cell death.

Graft compatibility is the capacity of two plants to form cohesive vascular connections. Tomato and pepper are incompatible graft partners; however, the underlying cause of graft rejection between these two species remains unknown. We diagnosed graft incompatibility between tomato and diverse pepper varieties based on weakened biophysical stability, decreased growth, and persistent cell death using viability stains.

Whole genome analysis of multidrug-resistant isolate collected from drinking water in Armenia revealed the plasmid-borne mediated colistin resistance.

Unlabelled: The rate of polymyxin-resistant , as well as human and animal infections caused by them, is increasing worldwide, posing a high epidemiological threat since colistin represents a last-resort antibiotic to treat complicated infections. The study of environmental niches, in particular, aquatic ecosystems in terms of genome analysis of inhabiting antimicrobial-resistant (AMR) microorganisms as reservoirs of acquired resistance determinants (AMR genes), represents a specific concern from a One Health approach. Here, we present a phenotypic AMR analysis and molecular characterization of isolate found in municipal drinking water after an accident in the water supply system of a residential building in Armenia in 2021.

Telemedicine is now legal in Armenia, how to make it a routine clinical practice?

Armenia's early adoption of telemedicine began with the National Aeronautics and Space Administration (NASA) collaboration following the 1988 Spitak earthquake, providing critical medical support. The 2022 legalization of telemedicine by the Armenian Ministry of Health marked a significant step toward improving healthcare delivery. Current regulations only apply to doctors and lack clear terminology for different telemedicine interactions, highlighting the need for broader and more inclusive policies.

Compositional-geochemical characterization of lead (Pb) anomalies and Pb-induced human health risk in urban topsoil.

Urban areas are characterized by a constant anthropogenic input, which is manifested in the chemical composition of the surface layer of urban soil. The consequence is the formation of intense anomalies of chemical elements, including lead (Pb), that are atypical for this landscape. Therefore, this study aims to explore the compositional-geochemical characteristics of soil Pb anomalies in the urban areas of Yerevan, Gyumri, and Vanadzor, and to identify the geochemical associations of Pb that emerge under prevalent anthropogenic influences in these urban areas.

Graft incompatibility between pepper and tomato can be attributed to genetic incompatibility between diverged immune systems.

Graft compatibility is the capacity of two plants to form cohesive vascular connections. Tomato and pepper are incompatible graft partners; however, the underlying cause of graft rejection between these two species remains unknown.We diagnosed graft incompatibility between tomato and diverse pepper varieties based on weakened biophysical stability, decreased growth, and persistent cell death using trypan blue and TUNEL assays.

Assessing the Adoption of One Health Approaches in National Plans to Combat Health Threats: The Pilot of a One Health Conceptual Framework in Armenia.

Due to several factors, such as environmental and climate changes, the risk of health threats originating at the human-animal-environment interface, including vector-borne diseases (VBDs) and zoonoses, is increasing. Low-resource settings struggle to counter these multidimensional risks due to their already-strained health systems and are therefore disproportionally affected by the impact caused by these changes. Systemic approaches like One Health (OH) are sought to strengthen prevention and preparedness strategies by addressing the drivers of potential threats with a multidisciplinary and multisectoral approach, considering the whole system at the human-animal-environment interface.

Bulky, electron-rich, renewable: analogues of Beller's phosphine for cross-couplings.

In recent years, considerable progress has been made in the conversion of biomass into renewable chemicals, yet the range of value-added products that can be formed from biomass remains relatively small. Herein, we demonstrate that molecules available from biomass serve as viable starting materials for the synthesis of phosphine ligands, which can be used in homogeneous catalysis. Specifically, we prepared renewable analogues of Beller's ligand (di(1-adamantyl)--butylphosphine, cataCXium® A), which is widely used in homogeneous catalysis.

Dirac points, new photonic band gaps, and effect of magnetically induced transparency in dichroic cholesteric liquid crystals with wavelength-dependent magneto-optical activity parameter.

We investigated the properties of cholesteric liquid crystals (CLCs) being in an external static magnetic field directed along the helix axis. We considered a dichroic CLC, that is, CLC with parameters ReΔ=Reɛ_{1}-Reɛ_{2}/2=0 and ImΔ=Imɛ_{1}-Imɛ_{2}/2≠0, where ɛ_{1,2} are the principal values of the local dielectric permittivity tensor. We have shown that in the case of the wavelength dependence of the magneto-optic activity parameter, new features appear in the optics of dichroic CLCs, in particular, in this case new Dirac points appear.

Development and results of the epilepsy surgery in Armenia: hope for a better future.

Purpose: We present our experience with the national epilepsy surgery program in Armenia by tracing the development of epilepsy surgery in the largest pediatric neurology department at "Arabkir" Medical Center. This development was possible on the basis of a strong collaboration with the Epilepsy Surgery center at the University Hospital "Sofia St. Ivan Rilski," Sofia, Bulgaria.

Anatomical and biophysical basis for graft incompatibility within the Solanaceae.

Interspecies grafting is an economically relevant technique that allows beneficial shoot and root combinations from separate species to be combined. One hypothesis for the basis of graft compatibility revolves around taxonomic relatedness. To test how phylogenetic distance affects interspecific graft compatibility within the economically important Solanaceae subfamily, Solanoideae, we characterized the anatomical and biophysical integrity of graft junctions between four species: tomato (Solanum lycopersicum), eggplant (Solanum melongena), pepper (Capsicum annuum), and groundcherry (Physalis pubescens).

Magnetically induced transparency in helically structured periodic crystals.

We investigated the specific properties of magnetically induced transparency (MIT) and magnetically induced absorption (MIA) in helically structured periodic crystals (HSPCs). We showed that for the wavelength of MIT we have an ideal optical diode: The forward signal passes fully, while the backward signal is completely absorbed and not reflected. A formula for the wavelength λ_{t} of MIT and MIA resonance based on the numerical simulations was analytically obtained.

Multimode Robust Lasing in a Dye-Doped Polymer Layer Embedded in a Wedge-Shaped Cholesteric.

Cholesteric liquid crystals (CLCs) with induced defects are one of the most prominent materials to realize compact, low-threshold and tunable coherent light sources. In this context, the investigation of optical properties of induced defect modes in such CLCs is of great interest. In particular, many studies have been devoted to the spectral control of the defect modes depending on their thickness, optical properties, distribution along the CLC, etc.

Magnetically induced linear,nonreciprocal, and tunable transparency.

We report the theoretical discovery of a new effect, namely, the effect of magnetically induced transparency. The effect is observed in a magnetically active helically structured periodical medium. Changing the external magnetic field and absorption, one can tune the frequency and the linewidth of the transparency band.

Magnetically Induced Transparency in Media with Helical Dichroic Structure.

In our paper, the magneto-optical properties of a dichroic cholesteric liquid crystal layer with large values of magneto-optical parameter g and low values of dielectric permittivity were investigated. The solutions of the dispersion equation and their peculiarities were investigated in detail. The specific properties of reflection, transmission, absorption, rotation, ellipticity spectra and also the spectra of ellipticity and azimuth of eigen polarization were investigated.

[Cancers of the external genital organs of male in Hérault: Results from the Hérault tumor register (RTH) over a period of 30 years (1987-2016)].

Aim: The objective of this study is to present the history of cancers of the external genital organs of male in Hérault using data from the Hérault tumor register (RTH) over a period of 30 years.

Patients And Methods: Using the RTH database, we studied the development of testicular germ cell tumors (TGCT) and penile cancer (PC) over 30 years, from 1987 to 2016. We analyzed the incidence and mortality data for these tumors.

CSO (Canadian Society of Otolaryngology - Head & Neck Surgery) position paper on rhinologic and skull base surgery during the COVID-19 pandemic.

Healthcare services in many countries have been partially or completely disrupted by the Coronavirus (COVID-19) pandemic since its onset in the end of 2019. Amongst the most impacted are the elective medical and surgical services in order to conserve resources to care for COVID-19 patients. As the number of infected patients decrease across Canada, elective surgeries are being restarted in a staged manner.

Non-canonical Targets of HIF1a Impair Oligodendrocyte Progenitor Cell Function.

Mammalian cells respond to insufficient oxygen through transcriptional regulators called hypoxia-inducible factors (HIFs). Although transiently protective, prolonged HIF activity drives distinct pathological responses in different tissues. Using a model of chronic HIF1a accumulation in pluripotent-stem-cell-derived oligodendrocyte progenitors (OPCs), we demonstrate that HIF1a activates non-canonical targets to impair generation of oligodendrocytes from OPCs.

[RHESOU (Registry in HErault Specialized in Onco-Urology) : the first French Registry specialized in Onco-Urology. One-year experience].

Purpose: In 2016, the Herault tumor registry collected 1961cancers in urology (21.4 % from all Herault cancers this year). RHESOU was created to complete RTH' data with specific parameters in onco-urology.

Suppression of proteolipid protein rescues Pelizaeus-Merzbacher disease.

Mutations in PLP1, the gene that encodes proteolipid protein (PLP), result in failure of myelination and neurological dysfunction in the X-chromosome-linked leukodystrophy Pelizaeus-Merzbacher disease (PMD). Most PLP1 mutations, including point mutations and supernumerary copy variants, lead to severe and fatal disease. Patients who lack PLP1 expression, and Plp1-null mice, can display comparatively mild phenotypes, suggesting that PLP1 suppression might provide a general therapeutic strategy for PMD.

Single cell-based fluorescence lifetime imaging of intracellular oxygenation and metabolism.

Oxidation-reduction chemistry is fundamental to the metabolism of all living organisms, and hence quantifying the principal redox players is important for a comprehensive understanding of cell metabolism in normal and pathological states. In mammalian cells, this is accomplished by measuring oxygen partial pressure (pO) in parallel with free and enzyme-bound reduced nicotinamide adenine dinucleotide (phosphate) [H] (NAD(P)H) and flavin adenine dinucleotide (FAD, a proxy for NAD). Previous optical methods for these measurements had accompanying problems of cytotoxicity, slow speed, population averaging, and inability to measure all redox parameters simultaneously.

High-throughput protein precipitation method with 96-well plate for determination of doxepin and nordoxepin in human plasma using LC-MS/MS.

The aim of this study was to establish a high-throughput and sensitive LC-MS/MS method for the determination of doxepin and its major active metabolite nordoxepin in human plasma. It has been designed for bioequivalence study for formulations containing 25 mg of doxepin. Doxepin and nordoxepin were extracted from human plasma samples by protein precipitation with acetonitrile by using protein precipitation 96-well plates.